CEACAM1/CD66a Antibody (283324) [Unconjugated]

Images

 
Western blot shows lysates of human liver tissue and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CEACAM‑1/CD66a Monoclonal Antibody (Catalog # MAB22441) ...read more
CEACAM‑1/CD66a was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human CEACAM‑1/CD66a Monoclonal Antibody (Catalog # MAB22441) at 25 µg/mL over­night at 4 ...read more
CEACAM‑1/CD66a was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human CEACAM‑1/CD66a Monoclonal Antibody (Catalog # MAB22441) at 25 µg/mL overnight at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ELISA(Cap)
Clone
283324
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

CEACAM1/CD66a Antibody (283324) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human CEACAM-1
Gln35-Gly428
Accession # P13688
Specificity
Detects human CEACAM-1 in ELISAs and Western blots. In ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CD31, rhICAM-1, -2, -3, recombinant mouse MAdCAM-1, or rhVCAM-1 was observed. In sandwich ELISAs, no cross-reactivity with rhCEACAM‑3, rhCEACAM-5, or rhCEACAM-6 was observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CEACAM1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Detection: Human CEACAM-1/CD66a Biotinylated Antibody (Catalog number BAF2244)
Standard: Recombinant Human CEACAM-1/CD66a (Catalog number 2244-CM)
Publications
Read Publications using
MAB22441 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CEACAM1/CD66a Antibody (283324) [Unconjugated]

  • antigen CD66
  • BGP1
  • BGP-1
  • BGP1BGPBiliary glycoprotein 1
  • BGPI
  • Biliary Glycoprotein 1
  • biliary glycoprotein adhesion molecule
  • carcinoembryonic antigen-related cell adhesion molecule 1 (biliaryglycoprotein)
  • carcinoembryonic antigen-related cell adhesion molecule 1
  • CD66a antigen
  • CD66a
  • Cea-1
  • CEACAM1
  • CEACAM-1
  • Hv-1
  • Hv-2
  • MHVR

Background

Carcinoembryonic antigen (CEA)-related cell adhesion molecule 1 (CEACAM-1; also called BGP and designated CD66a) is a 160 kDa member of the CEACAM branch of the CEA gene family of the immunoglobulin superfamily (1-3). It is one of seven human CEACAM subfamily genes that are essentially divided equally between type I transmembrane proteins (CEACAM-1, 3, and 4) and GPI-linked molecules (CEACAM-5-8). There is no CEACAM-2 in human. The gene for human CEACAM-1 codes for a 526 amino acid (aa) type I transmembrane protein that contains a 34 aa signal sequence, a 394 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 74 aa cytoplasmic region (4, 5). The ECD contains one N-terminal V-type Ig-like domain, followed by three C2-type Ig-like domains. It shows considerable glycosylation, including high mannose residues and (sialyl) LewisX (1). The cytoplasmic region shows one ITIM motif and a calmodulin binding site (1-3). In addition to the full length form, ten alternate splice forms have been reported (1, 4, 6-8). There are three soluble and seven transmembrane isoforms, with variations occurring in both the ECD and cytoplasmic region. All ten alternate splice forms contain the V‑type Ig-like domain (aa’s 35-142). The three soluble forms also contain the first two C2-type Ig-like domains (aa’s 145-317), with differences coming in the third C2-type Ig-like domain (6). The seven transmembrane isoforms are highly divergent. Five of the seven contain the V-type plus the first two C2-type domains and then diverge considerably both in the ECD and cytoplasmic region. The remaining two contain only the V-type Ig-like domain, the transmembrane region, and either a full-length or truncated cytoplasmic tail (1, 8). The actual functions of the isoforms are unclear. Full-length mouse and rat CEACAM-1 are approximately 57% aa identical to human CEACAM-1; in the V-type Ig-like domain, they are 58% and 56% aa identical, respectively. The full-length molecule is found on neutrophils, bile duct epithelium, activated NK cells, colonic columnar epithelium and endothelium. It is known to act as an intercellular adhesion molecule, forming both homotypic, and heterotypic bonds with CEA and CEACAM-6/NCA (3, 9). On neutrophils, CEACAM-1 also binds to dendritic cell CD-SIGN via its LeX moiety, inducing dendritic cell maturation and a subsequent Th1-type response (10,11).
  1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
  2. Thompson, J. et al. (1992) Genomics 12:761.
  3. Waggener, C. and S. Ergun (2000) Exp. Cell Res. 261:19.
  4. Barnett, T.R. et al. (1989) J. Cell Biol. 108:267.
  5. Hinoda, Y. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6959.
  6. Kuroki, M. et al. (1991) Biochem. Biophys. Res. Commun. 176:578.
  7. Barnett, T.R. et al. (1993) Mol. Cell. Biol. 13:1273.
  8. Watt, S.M. et al. (1994) Blood 84:200.
  9. Oikawa, S. et al. (1992) Biochem. Biophys. Res. Commun. 186:881.
  10. Klaas, P.J.M. et al. (2005) FEBS Lett. 579:6159.
  11. Bogoevska, V. et al. (2005) Glycobiology 16:197.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CEACAM1/CD66a Antibody (MAB22441)(2)

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Bioinformatics

Gene Symbol CEACAM1
Entrez
Uniprot