CEACAM8/CD66b Antibody [Unconjugated]

Human whole blood granulocytes were stained with Sheep Anti-Human CEACAM‑8/CD66b Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4246, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

CEACAM8/CD66b Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CEACAM‑8/CD66b
  Gln35-His141
  Accession # P31997
• Specificity: Detects human CEACAM‑8/CD66b in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) CEACAM-3, rhCEACAM-6, and rhCEACAM-7 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CEACAM8
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CEACAM8/CD66b Antibody [Unconjugated]

• Carcinoembryonic antigen CGM6
• carcinoembryonic antigen gene family member 6
• carcinoembryonic antigen-related cell adhesion molecule 8
• CD66b antigen
• CD66b
• CD67 antigen
• CD67
• CEACAM8
• CEACAM-8
• CGM6
• NCA-95
• Non-specific cross-reacting antigen NCA-95

Background

CEACAM-8 (carcinoembryonic antigen-related cell adhesion molecule 8; also CD66b, CD67 and NCA-95) is a 90 kDa member of the CEACAM subfamily of the CEA family of proteins. It is expressed by neutrophils and eosinophils, and serves as a binding partner for CEACAM-6 and Galectin-3. Mature human CEACAM-8 is a 287 amino acid GPI-linked glycoprotein. It contains one V-type and two C2-type Ig-like domains. No definitive rodent CEACAM-8 has been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CEACAM8/CD66b Antibody (AF4246)(1)

Publication using AF4246

• Kelly-Scumpia KM, Scumpia PO, Delano MJ, Weinstein JS, Cuenca AG, Wynn JL, Moldawer LL Type I interferon signaling in hematopoietic cells is required for survival in mouse polymicrobial sepsis by regulating CXCL10. J. Exp. Med., 2010-01-13;207(2):319-26. 2010-01-13 [PMID: 20071504] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CEACAM8
• Uniprot:
  • P31997()