CEACAM7 Antibody [Biotin]

Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human CEACAM‑7 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4478) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CEACAM‑7 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

CEACAM7 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human CEACAM-7
  Asn37-Phe142
  Accession # Q14002
• Specificity: Detects human CEACAM-7 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human CEACAM-1, -3, -4, -5,
  -6, and -8 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CEACAM7
• Purity: Antigen Affinity-purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Antigen Affinity-purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CEACAM7 Antibody [Biotin]

• Carcinoembryonic antigen CGM 2
• Carcinoembryonic antigen CGM2
• Carcinoembryonic antigen gene family member 2
• Carcinoembryonic antigen related cell adhesion molecule 7
• CEA
• CEACAM 7
• CEACAM7
• CEACAM-7
• CGM 2
• CGM2

Background

CEACAM-7 (carcinoembryonic antigen cell adhesion molecule 7; also CGM2) is a 40‑45 kDa member of the CEACAM subfamily of the CEA family of proteins. It has a restricted expression pattern, being found on epithelium of the colon, appendix and pancreatic ducts. It is markedly down‑regulated in colon carcinoma. Mature human CEACAM-7 is a 209 amino acid (aa), GPI-linked glycoprotein. It contains one V-type (aa 36‑142) and one C2-type (aa 146‑233) Ig-like domain, plus a 21 aa C‑terminal propeptide. One alternate splice form exists that shows a deletion of aa 143‑235. No definitive rodent CEACAM-7 has been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CEACAM7
• Uniprot:
  • Q14002(Human)