Home » CEACAM5/CD66e » CEACAM5/CD66e Antibodies » CEACAM5/CD66e Antibody (SPM541) [Janelia Fluor® 646]

CEACAM5/CD66e Antibody (SPM541) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
SPM541
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 646

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-34787AF350
Alexa Fluor 405
NBP2-34787AF405
Alexa Fluor 488
NBP2-34787AF488
Alexa Fluor 532
NBP2-34787AF532
Alexa Fluor 594
NBP2-34787AF594
Alexa Fluor 647
NBP2-34787AF647
Alexa Fluor 700
NBP2-34787AF700
Alexa Fluor 750
NBP2-34787AF750
Allophycocyanin
NBP2-34787APC
Biotin
NBP2-34787B
DyLight 350
NBP2-34787UV
DyLight 405
NBP2-34787V
DyLight 488
NBP2-34787G
DyLight 550
NBP2-34787R
DyLight 594
NBP2-34787DL594
DyLight 650
NBP2-34787C
DyLight 680
NBP2-34787FR
DyLight 755
NBP2-34787IR
FITC
NBP2-34787F
HRP
NBP2-34787H
Janelia Fluor 549
NBP2-34787JF549
PE
NBP2-34787PE
PerCP
NBP2-34787PCP
Unconjugated
NBP2-33013-0.1mg
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-33013-0.1mg
Azide and BSA Free
NBP2-34787-0.1 mg
IHC-Prediluted
NBP2-44902
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CEACAM5/CD66e Antibody (SPM541) [Janelia Fluor® 646] Summary

Immunogen
Human colon carcinoma extract
Localization
Cytoplasmic and luminal surface
Specificity
This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CEACAM5
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for CEACAM5/CD66e Antibody (SPM541) [Janelia Fluor® 646]

  • Carcinoembryonic antigen
  • carcinoembryonic antigen-related cell adhesion molecule 5
  • CD66e antigen
  • CD66e
  • CEA
  • CEACAM5
  • CEACAM-5
  • CEACD66e
  • DKFZp781M2392
  • Meconium antigen 100

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: MUC-1 Antibody (SM3) [NB120-22711] - IHC analysis of formalin fixed paraffin embedded tissue section of human breast cancer xenograft using MUC-1 antibody clone SM3 at 1:10 dilution. The xenograft section depicted a very specific and intense signal in the periphery of the cancer cells. The necrotic cells also developed a strong immune-positivity while the tumor stroma as well as the nuclei of cells were negative for immunostaining.
Immunocytochemistry/Immunofluorescence: MUC-1 Antibody (SM3) [NB120-22711] - MUC-1 antibody was tested in MCF7 cells at 10 ug/ml using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI.
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alpha-Fetoprotein/AFP ...
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Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
Western blot shows lysates of HepG2 human hepatocellular carcinoma parental cell line and alpha ‑Fetoprotein/AFP knockout HepG2 cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated) in the parental HeLacell line, but is not detectable in knockout HeLa cell line. GAPDH(Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
alpha -Fetoprotein/AFP knockout HepG2 hepatocellular carcinoma cell line was stained with Mouse Anti-Human alpha -Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by PE-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0102B). No staining in the alpha -Fetoprotein/AFP knockout HepG2 cell line was observed. View our protocol for Staining Membrane-associated Proteins.
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Immunocytochemistry/Immunofluorescence: TRIM26 Antibody [NBP2-47594] - Staining human cell line A-431 shows positivity in cytoplasm and nucleus but excluded from the nucleoli.
Immunohistochemistry-Paraffin: TRIM26 Antibody [NBP2-47594] - Staining of human tonsil shows strong cytoplasmic positivity in germinal center and non-germinal center cells.
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2/Neuron-specific Eno ...
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Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Analysis of different tissue and cell lysates using rabbit pAb to neuron specific enolase (NSE), NB110-58870, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH-3T3, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. A single band at about 47kDa corresponds to the NSE protein, seen only in extracts containing neurons ro neuronal lineage cells.
Immunocytochemistry/Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Analysis of mixed cortical neuron-glial cell culture from E20 rat stained with rabbit pAb to neuron specific enolase (NSE), NB110-58870, dilution 1:500 in red, and costained with chicken pAb to GFAP, dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. the NSE antibody labels protein expressed in neuronal cells, while the GFAP antibody stains intermediate filaments in astrocytic and certain other glial cells.
Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Blot of rat spinal cord probed with rabbit antibody to NSE. The antibody stains a single sharp band corresponing to NSE at about 47kDa.
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CSE1L/CAS/Exportin-2 A
H00001434-M08
Western Blot: CSE1L/CAS/Exportin-2 Antibody (3D8) [H00001434-M08] - Analysis of CSE1L expression in Hela S3 NE (Cat # L013V3).
Immunocytochemistry/Immunofluorescence: CSE1L/CAS/Exportin-2 Antibody (3D8) [H00001434-M08] - Analysis of monoclonal antibody to CSE1L on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: CSE1L/CAS/Exportin-2 Antibody (3D8) [H00001434-M08] - Analysis of monoclonal antibody to CSE1L on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml
(13 Publications)
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Antibody (OTI1A9)
NBP2-00532
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Antibody
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Simple Western: Cytokeratin 19 Antibody [NB100-687] - Lane view shows a specific band for Cytokeratin 19 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under standard reducing conditions using the 12-230 kDa separation system.
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Immunohistochemistry: Vimentin Antibody [NB300-223] - Increased numbers of differentiating NSPCs in SVZ of G93A-SOD1 brain. Confocal microscopy images of dorsal and ventral regions of SVZ in G93A-SOD1 and Wt-SOD1 brain at postnatal week 18, stained for nestin (green), GFAP (red), and vimentin (blue). Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (http://www.molecularbrain.com/content/8/1/5), licensed under a CC-BY licence.
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p53 Antibody (PAb
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[HRP]
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(1 Review)
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Antibody (44D9)
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(1 Review)
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Antibody (OTI1A12)
NBP2-45788
Western Blot: Serpin B3/SCCA1 Antibody (1A12) [NBP2-45788] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Serpin B3/SCCA1.
Immunohistochemistry: Serpin B3/SCCA1 Antibody (1A12) [NBP2-45788] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Serpin B3/SCCA1 Antibody (1A12) [NBP2-45788] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Cytokeratin 7
Antibody (OV-TL12/30) ...
NBP2-47940
Western Blot: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Antibody (OV-TL12/30).
Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Immunofluorescent staining of methanol-fixed HeLa cells. Cytokeratin 7 Antibody (OV-TL12/30) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Human Lung SCC stained with Cytokeratin 7 Monoclonal Antibody (OV-TL12/30)
(1 Review)
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Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, IF

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34787AF350
Alexa Fluor 405 NBP2-34787AF405
Alexa Fluor 488 NBP2-34787AF488
Alexa Fluor 532 NBP2-34787AF532
Alexa Fluor 594 NBP2-34787AF594
Alexa Fluor 647 NBP2-34787AF647
Alexa Fluor 700 NBP2-34787AF700
Alexa Fluor 750 NBP2-34787AF750
Allophycocyanin NBP2-34787APC
Biotin NBP2-34787B
DyLight 350 NBP2-34787UV
DyLight 405 NBP2-34787V
DyLight 488 NBP2-34787G
DyLight 550 NBP2-34787R
DyLight 594 NBP2-34787DL594
DyLight 650 NBP2-34787C
DyLight 680 NBP2-34787FR
DyLight 755 NBP2-34787IR
FITC NBP2-34787F
HRP NBP2-34787H
Janelia Fluor 549 NBP2-34787JF549
Janelia Fluor 646 NBP2-34787JF646
PE NBP2-34787PE
PerCP NBP2-34787PCP
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Bioinformatics

Gene Symbol CEACAM5