CEACAM5/CD66e Antibody (CEA31)

Images

 
Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) [NBP2-33012] - Human Colon Carcinoma stained with CEACAM5/CD66e Antibody (CEA31).
Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) [NBP2-33012] - Human colon stained with CEA MAb (CEA31)
Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) [NBP2-33012] - Human Colon Carcinoma stained with CEACAM5/CD66e Antibody (CEA31).
Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) [NBP2-33012] - Human Colon Carcinoma stained with CEACAM5/CD66e Antibody (CEA31).

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
CEA31
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

CEACAM5/CD66e Antibody (CEA31) Summary

Immunogen
Human colon carcinoma extract
Localization
Cytoplasmic and luminal surface
Specificity
This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CEACAM5
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25-0.5ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
  • Immunoprecipitation 0.5-1ug/500ug protein lysate
Application Notes
ELISA (For coating, order NBP2-34594 (BSA free version of NBP2-33012)). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A purified

Alternate Names for CEACAM5/CD66e Antibody (CEA31)

  • Carcinoembryonic antigen
  • carcinoembryonic antigen-related cell adhesion molecule 5
  • CD66e antigen
  • CD66e
  • CEA
  • CEACAM5
  • CEACAM-5
  • CEACD66e
  • DKFZp781M2392
  • Meconium antigen 100

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CEACAM5/CD66e Antibody (NBP2-33012). (Showing 1 - 2 of 2 FAQ).

  1. I recently ordered several antibodies (Cat No. NBP2-33010, NBP2-33011, NBP2-33012) to run in a FACS experiment. What concentration are the 0.5ml vials? It’s not listed on the vial, and I would like to start with the recommended FACS dilution (0.5-1ug/million cells) listed on the datasheet.
    • All of these three monoclonal antibodies have the concentration of 200 ug/ml. Therefore please use 2.5 - 5 ul of each antibody solution per 1 million cells tested in flow. 
  2. I would like to know the concentration of NBP2-33012.
    • The concentration of NBP2-33012 is 0.2mg/ml.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34594AF350
Alexa Fluor 405 NBP2-34594AF405
Alexa Fluor 488 NBP2-34594AF488
Alexa Fluor 532 NBP2-34594AF532
Alexa Fluor 594 NBP2-34594AF594
Alexa Fluor 647 NBP2-34594AF647
Alexa Fluor 700 NBP2-34594AF700
Alexa Fluor 750 NBP2-34594AF750
Allophycocyanin NBP2-34594APC
Allophycocyanin/Cy7 NBP2-34594APCCY7
Biotin NBP2-34594B
DyLight 350 NBP2-34594UV
DyLight 405 NBP2-34594V
DyLight 488 NBP2-34594G
DyLight 550 NBP2-34594R
DyLight 594 NBP2-34594DL594
DyLight 650 NBP2-34594C
DyLight 680 NBP2-34594FR
DyLight 755 NBP2-34594IR
FITC NBP2-34594F
HRP NBP2-34594H
Janelia Fluor 549 NBP2-34594JF549
Janelia Fluor 646 NBP2-34594JF646
PE NBP2-34594PE
PE/Atto594 NBP2-34594PEATT594
PE/Cy5.5 NBP2-34594PECY55
PE/Cy7 NBP2-34594PECY7
PerCP NBP2-34594PCP

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Bioinformatics Tool for CEACAM5/CD66e Antibody (NBP2-33012)

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