CEACAM5/CD66e Antibody (CB30) - BSA Free

Immunohistochemistry-Paraffin: CEACAM-5/CD66e Antibody (CB30) [NB110-58734] - Human colorectal carcinoma

Immunohistochemistry-Paraffin: CEACAM-5/CD66e Antibody (CB30) [NB110-58734] - Human colorectal carcinoma

• Reactivity: Hu
• Applications: Flow, IHC, IP, CyTOF-ready
• Clone: CB30
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CEACAM5/CD66e Antibody (CB30) - BSA Free Summary

• Immunogen: Human carcinoembryonic antigen (CEA; CEACAM5)
• Specificity: The antibody CB30 recognizes CD66e (CEA; 180-200 kDa), a cell surface bound carcinoembryonic antigen mainly expressed on epithelial cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CEACAM5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Immunoprecipitation 1:50
• Application Notes: For paraffin sections use standard ABC technique with pretreatment in 0.1% pepsin in 0.1M HCl for 30 minutes at RT. This antibody is CyTOF ready.
• Theoretical MW: 200 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for CEACAM5/CD66e Antibody (CB30) - BSA Free

• Carcinoembryonic antigen
• carcinoembryonic antigen-related cell adhesion molecule 5
• CD66e antigen
• CD66e
• CEA
• CEACAM5
• CEACAM-5
• CEACD66e
• DKFZp781M2392
• Meconium antigen 100

Background

The CD66e (CEA; 180-200 kDa) is a member of carcinoembryonic antigens, immunoglobulin supergene family and consists of a single N domain (structural homology to the immunoglobulin variable) and six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3). Human CD66e is heavily glycosylated GPI anchored protein capable of both homophilic and heterophilic adhesion. Disease relevance: The CD66e may play a role in the process of metastasis of cancer cells. CD66e is found in serum and it is clinically used as a tumor marker for early detection of disease due to its expression in adenocarcinomas potential target of tumor imaging and drug targeting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CEACAM5/CD66e Antibody (NB110-58734)(1)

Publication using NB110-58734

• Kim YJ, Li W, Zhelev DV et al. Chimeric antigen receptor-T cells are effective against CEACAM5 expressing non-small cell lung cancer cells resistant to antibody-drug conjugates Frontiers in Oncology 2023-02-27 [PMID: 36923424] (Immunohistochemistry)

Bioinformatics

• Gene Symbol: CEACAM5
• Entrez:
  • 1048(Human)