CDX2 Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CDX2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CDX2
  Asn54-Thr185
  Accession # Q99626
• Specificity: Detects human CDX2 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CDX2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CDX2 Antibody [Unconjugated]

• caudal type homeo box transcription factor 2
• caudal type homeobox 2
• Caudal-type homeobox protein 2
• CDX2
• CDX3
• CDX-3
• CDX3caudal type homeobox transcription factor 2
• homeobox protein CDX-2

Background

Human CDX2 (Caudal type homeobox transcription factor 2) is a 37 kDa member of the caudal family of homeobox proteins. It is 313 amino acids (aa) in length and contains a 167 aa caudal-like activation domain (aa 13‑180), a DNA-binding homeodomain (aa 186‑245) and two short, poly-glutamine and -proline repeats. CDX2 can exist as a dimer and is regulated by phosphorylation of a 4(x)serine motif that promotes CDX2 degradation. CDX2 promotes intestinal patterning and development. While it is necessary but not sufficient for intestinal phenotype differentiation, it will block cell proliferation on its own. Over the amino acid range used for immunization, human CDX2 is 95% and 97% aa identical to mouse and canine CDX2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CDX2 Antibody (AF3665)(7)

Publications using AF3665

• Zhou, C;Wang, M;Zhang, C;Zhang, Y; The transcription factor GABPA is a master regulator of naïve pluripotency bioRxiv : the preprint server for biology 2024-11-21 [PMID: 39605507] (Immunohistochemistry, Mouse)
• Moerkens, R;Mooiweer, J;Ramírez-Sánchez, AD;Oelen, R;Franke, L;Wijmenga, C;Barrett, RJ;Jonkers, IH;Withoff, S; An iPSC-derived small intestine-on-chip with self-organizing epithelial, mesenchymal, and neural cells Cell reports 2024-06-20 [PMID: 38907996] (Bioassay, Human)
• Wang, S;Leng, L;Wang, Q;Gu, Y;Li, J;An, Y;Deng, Q;Xie, P;Cheng, C;Chen, X;Zhou, Q;Lu, J;Chen, F;Liu, L;Yang, H;Wang, J;Xu, X;Hou, Y;Gong, F;Hu, L;Lu, G;Shang, Z;Lin, G; A single-cell transcriptome atlas of human euploid and aneuploid blastocysts Nature genetics 2024-06-05 [PMID: 38839885] (Immunohistochemistry, Human)
• AC Silva, OB Matthys, DA Joy, MA Kauss, V Natarajan, MH Lai, D Turaga, AP Blair, M Alexanian, BG Bruneau, TC McDevitt Co-emergence of cardiac and gut tissues promotes cardiomyocyte maturation within human iPSC-derived organoids Cell Stem Cell, 2021-12-02;28(12):2137-2152.e6. 2021-12-02 [PMID: 34861147] (IHC, Human)
• Neta Degani, Yoav Lubelsky, Rotem Ben-Tov Perry, Elena Ainbinder, Igor Ulitsky Highly conserved and cis-acting lncRNAs produced from paralogous regions in the center of HOXA and HOXB clusters in the endoderm lineage PLOS Genetics 7/19/2021 [PMID: 34280202]
• E Naumovska, G Aalderink, C Wong Valen, K Kosim, A Nicolas, S Brown, P Vulto, KS Erdmann, D Kurek Direct On-Chip Differentiation of Intestinal Tubules from Induced Pluripotent Stem Cells Int J Mol Sci, 2020-07-14;21(14):. 2020-07-14 [PMID: 32674311] (ICC, Human)
• R Francis, H Guo, C Streutker, M Ahmed, T Yung, PB Dirks, HH He, TH Kim Gastrointestinal transcription factors drive lineage-specific developmental programs in organ specification and cancer Sci Adv, 2019-12-11;5(12):eaax8898. 2019-12-11 [PMID: 31844668] (IHC, Mouse)

Bioinformatics

• Gene Symbol: CDX2
• Uniprot:
  • Q99626()