CDO Antibody [Unconjugated]

C2C12 mouse myoblast cell line was stained with Sheep Anti-Human CDO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4384, filled histogram) or control antibody (Catalog # 5‑001‑A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).

CDO was detected in immersion fixed C2C12 mouse myoblast cell line using Sheep Anti-Human CDO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4384) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Jurkat cells were stained with Sheep Anti-Human CDO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4384, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

CDO Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CDO
  Asp26-Pro943 (Leu669Ile)
  Accession # NP_058648
• Specificity: Detects human CDO in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse CDO is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CDON
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CDO Antibody [Unconjugated]

• CDO
• Cdon homolog (mouse)
• CDON
• CDOORCAM
• cell adhesion molecule-related/down-regulated by oncogenes
• MGC111524
• ORCAM
• surface glycoprotein, Ig superfamily member

Background

CDO (CAM-related/down-regulated by oncogenes, also CDON; pronounced “kid-oh”) is a 190 kDa member of the Immunoglubulin (Ig) superfamily, Ig/Fibronectin (FN) type III repeat family of cell surface proteins (1). Human CDO is a type I transmembrane (TM) glycoprotein. It is synthesized as a 1287 amino acid (aa) precursor that contains a 25 aa signal sequence, a 938 aa extracellular domain (ECD), a 21 aa TM segment and a 303 aa cytoplasmic region (1, 2). The ECD contains five C2-type Ig-like domains, followed by three FN type III repeats. The first FN repeat (aa 577-673) is known to bind numerous cadherins, while the third (or juxtramembrane) FN type III repeat (aa 826-923) binds SHH (3, 4). The intracellular region is believed to signal through various bHLH transcription factors (2). One alternate splice form is reported that shows a deletion of aa 1212-1234 in the cytoplasmic tail. The ECD of human CDO is 85% aa identical to mouse CDO ECD. CDO is found on muscle precursor and neural progenitor cells of the embryo (5, 6). It likely promotes muscle differentiation, and contributes to axon guidance and neuronal patterning (2, 7, 8, 9). These effects may be mediated through two different receptor complexes. On muscle precursors, CDO apparently acts as both a coordinating and signaling subunit. Here, it integrates N- and M-cadherin, neogenin, netrin-3 and BOC into a cis-oriented receptor complex (2). While this complex has no identified ligand, intercellular cadherin interactions or netrin, may be enough to trigger CDO/cadherin/neogenin signaling. On axons, CDO may participate in a poorly-defined receptor complex minimally composed of CDO, BOC and Gas1 that binds SHH, and interacts with PTCH1 (7, 8, 10).

1. Kang, J.S. et al. (1997) J. Cell Biol. 138:203. 
2. Krauss, R.S. et al. (2005) J. Cell Sci. 118:2355. 
3. Yao, S. et al. (2006) Cell 125:343. 
4. Kang, J-S. et al. (2003) Proc. Natl. Acad. Sci. USA 100:3989. 
5. Kang, J-S. et al. (2002) EMBO J. 21:114. 
6. Zhang, W. et al. (2006) Mol. Cell. Biol. 26:3764.
7. Okada, A. et al. (2006) Nature 444:369.
8. Allen, B.L. et al. (2007) Genes Dev. 21:1244.
9. Kang, J-S. et al. (2004) J. Cell. Biol. 167:493.
10. Tenzen, T. et al. (2006) Dev. Cell 10:647.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CDO Antibody (AF4384)(2)

Publications using AF4384

• Cardozo M, Sanchez-Arrones L, Sandonis A, Sanchez-Camacho C, Gestri G, Wilson S, Guerrero I, Bovolenta P Cdon acts as a Hedgehog decoy receptor during proximal-distal patterning of the optic vesicle. Nat Commun, 2014-07-08;5(0):4272. 2014-07-08 [PMID: 25001599] (Western Blot, Human)
• Céline Delloye-Bourgeois, Benjamin Gibert, Nicolas Rama, Jean-Guy Delcros, Nicolas Gadot, Jean-Yves Scoazec et al. Sonic Hedgehog Promotes Tumor Cell Survival by Inhibiting CDON Pro-Apoptotic Activity PLoS Biology 2013-08-06 [PMID: 23940460] (Immunocytochemistry, Human)

Bioinformatics

• Gene Symbol: CDON
• Uniprot:
  • NP_058648()