CDO Antibody [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 405

CDO Antibody [Alexa Fluor® 405] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CDO
  Asp26-Pro943 (Leu669Ile)
  Accession # NP_058648
• Specificity: Detects human CDO in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse CDO is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CDO Antibody [Alexa Fluor® 405]

• CDO
• Cdon homolog (mouse)
• CDON
• CDOORCAM
• cell adhesion molecule-related/down-regulated by oncogenes
• MGC111524
• ORCAM
• surface glycoprotein, Ig superfamily member

Background

CDO (CAM-related/down-regulated by oncogenes, also CDON; pronounced “kid-oh”) is a 190 kDa member of the Immunoglubulin (Ig) superfamily, Ig/Fibronectin (FN) type III repeat family of cell surface proteins (1). Human CDO is a type I transmembrane (TM) glycoprotein. It is synthesized as a 1287 amino acid (aa) precursor that contains a 25 aa signal sequence, a 938 aa extracellular domain (ECD), a 21 aa TM segment and a 303 aa cytoplasmic region (1, 2). The ECD contains five C2-type Ig-like domains, followed by three FN type III repeats. The first FN repeat (aa 577-673) is known to bind numerous cadherins, while the third (or juxtramembrane) FN type III repeat (aa 826-923) binds SHH (3, 4). The intracellular region is believed to signal through various bHLH transcription factors (2). One alternate splice form is reported that shows a deletion of aa 1212-1234 in the cytoplasmic tail. The ECD of human CDO is 85% aa identical to mouse CDO ECD. CDO is found on muscle precursor and neural progenitor cells of the embryo (5, 6). It likely promotes muscle differentiation, and contributes to axon guidance and neuronal patterning (2, 7, 8, 9). These effects may be mediated through two different receptor complexes. On muscle precursors, CDO apparently acts as both a coordinating and signaling subunit. Here, it integrates N- and M-cadherin, neogenin, netrin-3 and BOC into a cis-oriented receptor complex (2). While this complex has no identified ligand, intercellular cadherin interactions or netrin, may be enough to trigger CDO/cadherin/neogenin signaling. On axons, CDO may participate in a poorly-defined receptor complex minimally composed of CDO, BOC and Gas1 that binds SHH, and interacts with PTCH1 (7, 8, 10).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • NP_058648()