Gas1 Antibody [Unconjugated]

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Gas1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Gas1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2644) at 5 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Gas1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Gas1
Leu39-Ser315
Accession # Q01721
Specificity
Detects mouse Gas1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human Gas1 and recombinant mouse Gas6 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GAS1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Mouse Gas1 Antibody (Catalog # AF2644)
ELISA Detection: Mouse Gas1 Biotinylated Antibody (Catalog # BAF2644)
Standard:Recombinant Mouse Gas1 (Catalog # 2644-GS)
Publications
Read Publications using
AF2644 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Gas1 Antibody [Unconjugated]

  • Gas1
  • GAS-1
  • growth arrest-specific 1
  • Growth arrest-specific gene-1
  • growth arrest-specific protein 1

Background

Gas1 (Growth Arrest Specific 1) is one of six structurally unrelated proteins that were identified by their increased expression in growth-arrested cells relative to actively proliferating cells (1, 2). Following mitogenic stimulation, Gas1 expression is transcriptionally suppressed by c-Myc as cells transit from G0 to G1 phases of the cell cycle (3, 4). Overexpression of Gas1 prevents S phase entry and DNA synthesis (5). Gas1-mediated blockade of the cell cycle is p53-dependent but does not require the transactivating domain of p53 (6). The mouse Gas1 cDNA encodes a 343 amino acid (aa) precursor that includes a 38 aa signal sequence, a 277 aa mature protein, and a 28 aa C-terminal propeptide. Gas1 contains Ala-rich and Asp-rich regions as well as an RGD sequence (5). Mature mouse and human Gas1 share 85% aa sequence identity. Mouse Gas1 is a 40 kDa GPI linked glycoprotein that is uniformly distributed on the cell surface (7). In contact inhibited vascular endothelial cells, Gas1 is induced by VE-Cadherin and VEGF expression and mediates the anti-apoptotic effect of VEGF (8). In contrast, Gas1 is induced in hippocampal neurons after NMDA exposure but functions as a pro-apoptotic effector of NMDA-mediated excitotoxicity (9). Gas1 exhibits a range of developmental effects including either promoting or inhibiting growth and differentiation of somite, limb, cerebellar, and eye tissues (10‑14). Gas1 mediates the antagonistic effect of Wnt proteins toward Shh function by binding the N-terminal region of Shh (11). The dependence of Gas1 functions on the cellular context has been addressed by suggesting that Gas1 could function as a co-receptor for GDNF family ligands (15). This speculation is supported by R&D Systems’ data that demonstrate direct binding of Gas1 to Artemin and Neurturin.

  1. Schneider, C. et al. (1988) Cell 54:787.
  2. Mullor, J.L. and A.R. Altaba (2002) BioEssays 24:22.
  3. Del Sal, G. et al. (1994) Proc. Natl. Acad. Sci. USA 91:1848.
  4. Lee, T.C. et al. (1997) Proc. Natl. Acad. Sci. USA 94:12886.
  5. Del Sal, G. et al. (1992) Cell 70:595.
  6. Del Sal, G. et al. (1995) Mol. Cell. Biol. 15:7152.
  7. Stebel, M. et al. (2000) FEBS Lett. 481:152.
  8. Spagnuolo, R. et al. (2004) Blood 103:3005.
  9. Mellstrom, B. et al. (2002) Mol. Cell Neurosci. 19:417.
  10. Lee, K.K.H. et al. (2001) Dev. Biol. 234:188.
  11. Lee, C.S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11347.
  12. Liu, Y. et al. (2002) Development 129:5289.
  13. Liu, Y. et al. (2001) Dev. Biol. 236:30.
  14. Lee, C.S. et al. (2001) Dev. Biol. 236:17.
  15. Schueler-Furman, O. et al. (2006) Trends Pharmacol. Sci. 27:72.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Gas1 Antibody (AF2644)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC, IHC-P, Western Blot.


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(1)
IHC-P
(1)
Western Blot
(2)
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(1)
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(3)
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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF2644

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Bioinformatics

Gene Symbol GAS1
Uniprot