BOC Antibody (273729)

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 273729
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

BOC Antibody (273729) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human BOC
  Asp31-Asp852
  Accession # Q9BWV1
• Specificity: Detects human and mouse BOC in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: BOC
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BOC Antibody (273729)

• Boc homolog (mouse)
• BOC
• brother of CDO
• Protein BOC

Background

BOC (Brother of CDO [CAM-related/down-regulated by oncogenes]) is a member of the Immunoglobulin (Ig) superfamily, Ig/Fibronectin (FN) type III repeat family of cell surface proteins (1). Human BOC is a type I transmembrane (TM) protein. It is synthesized as a 1114 amino acid (aa) precursor that contains a 30 aa signal sequence, an 825 aa extracellular domain (ECD), a 21 aa TM segment and a 238 aa cytoplasmic region (1, 2). The ECD contains four Ig-like domains, followed by three FN type III repeats. The third (or juxtramembrane) FN type III repeat (aa 712‑809) binds SHH (3). The intracellular region is not essential for BOC-containing receptor complex signaling (1). However, it appears both the ECD and intracellular regions of BOC are used to form functional subunit interactions in cis-oriented receptor complexes (1, 4). One 157 aa BOC alternate splice form is reported that shows a 32 aa substitution for aa 126‑1114. The ECD of human BOC is 92% aa identical to mouse BOC ECD. BOC is found in the embryo associated with muscle precursors, limb mesenchyme, early chondrocytes and neurons (2, 5, 6). It appears to promote muscle differentiation and axon guidance (2, 6). BOC contributes to two multi-subunit receptor complexes. On myocytes, a BOC-associated complex includes CDO, neogenin, netrin, and at least two cadherin homodimers formed by either M- or N-cadherin (2). A second complex on neurons, somewhat ill‑defined, potentially includes BOC, CDO and Gas1. Here, BOC and/or CDO interact with SHH, with subsequent “transfer” or presentation of SHH to PTCH1 (6, 7).

1. Kang, J.-S. et al. (2002) EMBO J. 21:114.
2. Krauss, R.S. et al. (2005) J. Cell Sci. 118:2355.
3. Yao, S. et al. (2006) Cell 125:343.
4. Kang, J.-S. et al. (2003) Proc. Natl. Acad. Sci. USA 100:3989.
5. Mulieri, P.J. et al. (2002) Dev. Dyn. 223:379.
6. Okada, A. et al. (2006) Nature 444:369.
7. Allen, B.L. et al. (2007) Genes Dev. 21:1244.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BOC Antibody (MAB20361)(4)

Publications using MAB20361

• Lee, J;Kim, Y;Ataliotis, P;Kim, HG;Kim, DW;Bennett, DC;Brown, NA;Layman, LC;Kim, SH; Coordination of canonical and noncanonical Hedgehog signalling pathways mediated by WDR11 during primordial germ cell development Scientific reports 2023-07-29 [PMID: 37516749] (Western Blot, Mouse)
• Y Kim, J Lee, M Seppala, MT Cobourne, SH Kim Ptch2/Gas1 and Ptch1/Boc differentially regulate Hedgehog signalling in murine primordial germ cell migration Nat Commun, 2020-04-24;11(1):1994. 2020-04-24 [PMID: 32332736] (IHC, Mouse)
• Rebekah S. Cosden-Decker, Melissa M. Bickett, Christian Lattermann, James N. MacLeod Structural and functional analysis of intra-articular interzone tissue in axolotl salamanders Osteoarthritis and Cartilage 11/1/2012 [PMID: 22800772]
• Rebekah S. Cosden-Decker, Melissa M. Bickett, Christian Lattermann, James N. MacLeod Structural and functional analysis of intra-articular interzone tissue in axolotl salamanders Osteoarthritis and Cartilage 2012-11-01 [PMID: 22800772] (Immunohistochemistry, Equine, Mouse, Amphibian - Ambystoma mexicanum (Axolotl))

Bioinformatics

• Gene Symbol: BOC
• Uniprot:
  • Q9BWV1()