CDC5L Antibody

Immunoprecipitation: CDC5L Antibody [NB100-87013] - Detection of Human CDC5L by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDC5L antibody NB100-87013 used for IP at 3 mcg/mg lysate. CDC5L was also immunoprecipitated by rabbit anti- CDC5L antibody NB100-87014, which recognizes a downstream epitope.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: IP, WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

CDC5L Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human cell division cycle 5 (CDC5) -like using the numbering given in entry NP_001244.1 (GeneID988).
• Predicted Species: Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDC5L
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2- 5 ug/mg lysate
• Application Notes: CDC5L antibody validated for IP from a verified customer review. Western Blot Not recommended

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CDC5L Antibody

• CDC5 (cell division cycle 5, S. pombe, homolog)-like
• CDC5 cell division cycle 5-like (S. pombe)
• CDC5
• Cdc5-like protein
• Cdc5-related protein
• CEF1
• cell division cycle 5-like protein
• dJ319D22.1 (CDC5-like protein)
• dJ319D22.1
• hCDC5
• KIAA0432
• PCDC5RPCDC5-LIKE
• Pombe cdc5-related protein

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for CDC5L Antibody (NB100-87013) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Chuck David IP Human 10/18/2010

Summary

• Application: Immunoprecipitation
• Sample Tested: HeLa NE
• Species: Human
• Lot: A1

Bioinformatics

• Gene Symbol: CDC5L
• Entrez:
  • 988(Human)
• Uniprot:
  • NP_001244.1(Human)