CDC5L Antibody

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Immunoprecipitation: CDC5L Antibody [NB100-87013] - Detection of Human CDC5L by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications IP, WB (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CDC5L Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human cell division cycle 5 (CDC5) -like using the numbering given in entry NP_001244.1 (GeneID988).
Predicted Species
Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDC5L
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 2- 5 ug/mg lysate
Application Notes
CDC5L antibody validated for IP from a verified customer review. Western Blot Not recommended
Reviewed Applications
Read 1 Review rated 4
using
NB100-87013 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CDC5L Antibody

  • CDC5 (cell division cycle 5, S. pombe, homolog)-like
  • CDC5 cell division cycle 5-like (S. pombe)
  • CDC5
  • Cdc5-like protein
  • Cdc5-related protein
  • CEF1
  • cell division cycle 5-like protein
  • dJ319D22.1 (CDC5-like protein)
  • dJ319D22.1
  • hCDC5
  • KIAA0432
  • PCDC5RPCDC5-LIKE
  • Pombe cdc5-related protein

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CDC5L Antibody (NB100-87013) (0)

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Review for CDC5L Antibody (NB100-87013) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-87013:
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IP
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
IP Human 10/18/2010
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Summary

ApplicationImmunoprecipitation
Sample TestedHeLa NE
SpeciesHuman
LotA1
CommentsThis antibody works well for IP. I have not tried it for western (it is not recommended for western). Protein Capture: Protein A agarose

Comments

CommentsThis antibody works well for IP. I have not tried it for western (it is not recommended for western). Protein Capture: Protein A agarose

Product General Protocols

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FAQs for CDC5L Antibody (NB100-87013) (0)

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Secondary Antibodies

 

Isotype Controls

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Verified Customer
10/18/2010
Application: IP
Species: Human

Bioinformatics

Gene Symbol CDC5L
Entrez
Uniprot