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PLRG1 Antibody

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 (1 Review)
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Western Blot: PLRG1 Antibody [NBP1-06556] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. NBP1-06556 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate.
Independent Antibodies: Western Blot: PLRG1 Antibody [NBP1-06556] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. ...read more
Immunocytochemistry/ Immunofluorescence: PLRG1 Antibody [NBP1-06556] - human breast carcinoma. Antibody dilution of 1:100.
Immunohistochemistry: PLRG1 Antibody [NBP1-06556] - Sections of human prostate carcinoma (left) and breast carcinoma (right). Antibody: Affinity purified rabbit anti- PLRG1 used at a dilution of 1:200 (1ug/ml). ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
 

Independent Antibodies

  

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PLRG1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 464 and 514 of human pleiotropic regulator 1 using the numbering given in entry NP_002660.1 (GeneID 5356).
Predicted Species
Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLRG1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-06556 in the following applications:
Publications
Read Publications using NBP1-06556.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PLRG1 Antibody

  • MGC110980
  • pleiotropic regulator 1 (PRL1 homolog, Arabidopsis)
  • PRL1Arabidopsis homolog)

Background

Pleiotropic regulator 1 (PLRG1) has been shown to be a part of sub-spliceosomal protein complexes. It is highly conserved across species and is essential for pre-mRNA splicing. PLRG1 interacts with human CDC5. This interaction has also been shown to be essential to pre-mRNA splicing. PLRG1 is also known as PRL1, and MGC110980.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: CDC5L Antibody (JJ091-05) [NBP2-67901] - Analysis of CDC5L on different lysates using anti-CDC5L antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat
Immunocytochemistry/Immunofluorescence: CDC5L Antibody (JJ091-05) [NBP2-67901] - Staining CDC5L in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: CDC5L Antibody (JJ091-05) [NBP2-67901] - Analysis of paraffin-embedded mouse lung tissue using anti-CDC5L antibody. Counter stained with hematoxylin.
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Applications: WB, ICC/IF, IHC, IHC-P
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Western Blot: PRP19 Antibody (SC06-53) [NBP2-67597] - Analysis of PRP19 on Jurkat cells lysates using anti-PRP19 antibody at 1/1,000 dilution.
Immunocytochemistry/Immunofluorescence: PRP19 Antibody (SC06-53) [NBP2-67597] - Staining PRP19 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: PRP19 Antibody (SC06-53) [NBP2-67597] - Analysis of paraffin-embedded mouse kidney tissue using anti-PRP19 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
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Western Blot: PRL-2/PTP4A2 Antibody [NBP2-93937] - Analysis of extracts of various cell lines, using PRL-2/PTP4A2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/Immunofluorescence: PRL-2/PTP4A2 Antibody [NBP2-93937] - Analysis of C6 cells using PRL-2/PTP4A2 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: PRL-2/PTP4A2 Antibody [NBP2-93937] - Paraffin-embedded rat kidney using PRL-2/PTP4A2.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
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Western Blot: PBX1 Antibody (4A2) [H00005087-M01] - PBX1 monoclonal antibody (M01), clone 4A2 Analysis of PBX1 expression in Hela S3 NE.
Immunocytochemistry/Immunofluorescence: PBX1 Antibody (4A2) [H00005087-M01] - Analysis of monoclonal antibody to PBX1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: PBX1 Antibody (4A2) [H00005087-M01] - Analysis of monoclonal antibody to PBX1 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.
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Western Blot: BCAS2 Antibody [NB110-40681] - Detection of Human and Mouse BCAS2 by Western Blot. Samples: Whole cell lysate from MCF7 (5, 15 and 50 ug), and HeLa (50ug), and mouse NIH3T3 (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BCAS2 antibody NB110-40681 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: BCAS2 Antibody [NB110-40681] - Mouse plasmacytoma. Antibody: Affinity purified rabbit anti-BCAS2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.
Immunohistochemistry-Paraffin: BCAS2 Antibody [NB110-40681] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BCAS2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.
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Western blot shows lysates of human, mouse, and rat pituitary gland tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Growth Hormone at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.
Species: Hu, Mu, Rt, Pm
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Immunocytochemistry/Immunofluorescence: PPP2R4 Antibody (4D9) [NBP2-37530] - Immunofluorescence analysis of Hela cells using PPP2R4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry: PPP2R4 Antibody (4D9) [NBP2-37530] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using PPP2R4 mouse mAb with DAB staining.
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SDS-Page: Recombinant Human gamma-glutamyl hydrolase Protein [NBP1-78898]
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Immunocytochemistry/Immunofluorescence: RBM22 Antibody [NBP1-85379] - Staining of human cell line U-251 MG shows localization to nucleus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RBM22 Antibody [NBP1-85379] - Staining of human tonsil shows strong nuclear positivity in non-germinal and germinal center cells.
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Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC2 in HCT116 p53-/- cells. Western blot of ORC2 in HBEC and 293T cell lines. Ponceau S staining of total protein or immunoblot of Chk1 show equal loading of the pairs of lanes. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/19084), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: Chk1 Antibody [NB100-464] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHK1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: Chk1 Antibody [NB100-464] - Detection of Human Chk1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TMCK cells. Antibodies: Affinity purified rabbit anti-Chk1 antibody NB100-464 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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ATP7b Antibody
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Western Blot: ATP7b Antibody [NB100-360] - ATP7b detected in 20 ug of mouse brain membrane fraction.
Immunocytochemistry/Immunofluorescence: ATP7b Antibody [NB100-360] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ATP7b [NB100-360] at a 1:100 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry: ATP7b Antibody [NB100-360] - Analysis of ATP7b in mouse liver using DAB with hematoxylin counterstain.
(1 Review)
(15 Publications)
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Immunocytochemistry/Immunofluorescence: Dicer Antibody [NBP1-71691] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Dicer at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Dicer Antibody [NBP1-71691] - Analysis of Dicer in paraffin embedded human colorectal cancer tissue using anti-Dicer antibody. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: Dicer Antibody [NBP1-71691] - Dicer antibody was tested in HeLa cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).
(1 Review)
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
c-jun Antibody
(5B1)
NBP2-37576
Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.
Immunocytochemistry/Immunofluorescence: c-jun Antibody (5B1) [NBP2-37576] - Analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: c-jun Antibody (5B1) [NBP2-37576] - Analysis of human rectum cancer tissues using c-Jun mouse mAb with DAB staining.
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P

Publications for PLRG1 Antibody (NBP1-06556)(2)


Review for PLRG1 Antibody (NBP1-06556) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-06556:
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(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
 IP Human 10/18/2010
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Summary

ApplicationImmunoprecipitation
Sample TestedHeLa NE
SpeciesHuman
LotA1
CommentsThis antibody works very well for IP. Using my conditions, the western signal was a little weak.

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 5 minutes, Blocking Temp: 23 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Incubation Dilution Buffer: TBST 5% milk, Primary Ab Dilution Ratio: 1:1000, Incubation Time:1hr, Incubation Temp:23C

Secondary Antibody

Secondary DescriptionSecondary Ab: Li-cor anti-rabbit, Secondary Ab Dilution: 1:10000

Details

Detection NotesDetection Method: Scanned using Li-cor system, Specific Bands: 55kDa, Non-Specific Bands: none

Comments

CommentsThis antibody works very well for IP. Using my conditions, the western signal was a little weak.

Product General Protocols

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WB Video Protocol
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FAQs for PLRG1 Antibody (NBP1-06556) (0)

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Recent Reviews

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 Anonymous
10/18/2010
Application: IP
Species: Human
read this review

Bioinformatics

Gene Symbol PLRG1
Entrez
Uniprot