PLRG1 Antibody

Western Blot: PLRG1 Antibody [NBP1-06556] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. NBP1-06556 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate.

Immunocytochemistry/Immunofluorescence: PLRG1 Antibody [NBP1-06556] - human breast carcinoma. Antibody dilution of 1:100.

Immunohistochemistry: PLRG1 Antibody [NBP1-06556] - Sections of human prostate carcinoma (left) and breast carcinoma (right). Antibody: Affinity purified rabbit anti- PLRG1 used at a dilution of 1:200 (1ug/ml). Detection: DAB and Redfluorescent goat anti-rabbit IgG-heavy and light chain,cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

PLRG1 Antibody Summary

• Description: Novus Biologicals Rabbit PLRG1 Antibody (NBP1-06556) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-PLRG1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 464 and 514 of human pleiotropic regulator 1 using the numbering given in entry NP_002660.1 (GeneID 5356).
• Predicted Species: Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PLRG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PLRG1 Antibody

• MGC110980
• pleiotropic regulator 1 (PRL1 homolog, Arabidopsis)
• PRL1Arabidopsis homolog)

Background

Pleiotropic regulator 1 (PLRG1) has been shown to be a part of sub-spliceosomal protein complexes. It is highly conserved across species and is essential for pre-mRNA splicing. PLRG1 interacts with human CDC5. This interaction has also been shown to be essential to pre-mRNA splicing. PLRG1 is also known as PRL1, and MGC110980.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PLRG1 Antibody (NBP1-06556)(2)

Publications using NBP1-06556

• David CJ, Boyne AR, Millhouse SR et al. The RNA polymerase II C-terminal domain promotes splicing activation through recruitment of a U2AF65-Prp19 complex. Genes Dev 2011-05-01 [PMID: 21536736]
• Nakashima M, Lazo JS. Phosphatase of regenerating liver-1 promotes cell migration and invasion and regulates filamentous actin dynamics. J Pharmacol Exp Ther 2010-08-01 [PMID: 20484558]

Review for PLRG1 Antibody (NBP1-06556) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IP Human 10/18/2010

Summary

• Application: Immunoprecipitation
• Sample Tested: HeLa NE
• Species: Human
• Lot: A1

Bioinformatics

• Gene Symbol: PLRG1
• Entrez:
  • 5356(Human)
  • 53317(Mouse)
• Uniprot:
  • O43660(Human)