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PLRG1 Antibody

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 (1 Review)
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Western Blot: PLRG1 Antibody [NBP1-06556] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. NBP1-06556 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate.
Independent Antibodies: Western Blot: PLRG1 Antibody [NBP1-06556] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. ...read more
Immunocytochemistry/ Immunofluorescence: PLRG1 Antibody [NBP1-06556] - human breast carcinoma. Antibody dilution of 1:100.
Immunohistochemistry: PLRG1 Antibody [NBP1-06556] - Sample: FFPE sections of human prostate carcinoma (left) and breast carcinoma (right). Antibody: Affinity purified rabbit anti- PLRG1 used at a dilution of 1:200 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
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PLRG1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 464 and 514 of human pleiotropic regulator 1 using the numbering given in entry NP_002660.1 (GeneID 5356).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLRG1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunoprecipitation 2-5 ug/mg lysate
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-06556 in the following applications:
Publications
Read Publications using NBP1-06556.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PLRG1 Antibody

  • MGC110980
  • pleiotropic regulator 1 (PRL1 homolog, Arabidopsis)
  • PRL1Arabidopsis homolog)

Background

Pleiotropic regulator 1 (PLRG1) has been shown to be a part of sub-spliceosomal protein complexes. It is highly conserved across species and is essential for pre-mRNA splicing. PLRG1 interacts with human CDC5. This interaction has also been shown to be essential to pre-mRNA splicing. PLRG1 is also known as PRL1, and MGC110980.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: PRL-2/PTP4A2 Antibody [NBP2-30075] - NCI-H460 cell line lysates (35ug/lane).This demonstrates the PTP4A2 antibody detected the PTP4A2 protein (arrow).
Immunocytochemistry/Immunofluorescence: PRL-2/PTP4A2 Antibody [NBP2-30075] - PTP4A2 Antibody (Center) with paraffin-embedded human prostate carcinoma tissue. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
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(1 Review)
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Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human tonsil tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for PLRG1 Antibody (NBP1-06556)(2)


Review for PLRG1 Antibody (NBP1-06556) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-06556:
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(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
 IP Human 10/18/2010
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Summary

ApplicationImmunoprecipitation
Sample TestedHeLa NE
SpeciesHuman
LotA1
CommentsThis antibody works very well for IP. Using my conditions, the western signal was a little weak.

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 5 minutes, Blocking Temp: 23 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Incubation Dilution Buffer: TBST 5% milk, Primary Ab Dilution Ratio: 1:1000, Incubation Time:1hr, Incubation Temp:23C

Secondary Antibody

Secondary DescriptionSecondary Ab: Li-cor anti-rabbit, Secondary Ab Dilution: 1:10000

Details

Detection NotesDetection Method: Scanned using Li-cor system, Specific Bands: 55kDa, Non-Specific Bands: none

Comments

CommentsThis antibody works very well for IP. Using my conditions, the western signal was a little weak.

Product General Protocols

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FAQs for PLRG1 Antibody (NBP1-06556) (0)

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Diseases for PLRG1 Antibody (NBP1-06556)

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Recent Reviews

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 Anonymous
10/18/2010
Application: IP
Species: Human
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Bioinformatics

Gene Symbol PLRG1
Entrez
Uniprot