CDC42 Antibody (3C3) - BSA Free

Immunohistochemistry: CDC42 Antibody (3C3) [NBP3-26670] - Image of CDC42 Antibody (3C3) diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Western Blot: CDC42 Antibody (3C3) [NBP3-26670] - Positive Western Blot detected in: NIH/3T3 whole cell lysate, K562 whole cell lysate, Mouse Brain whole cell lysate. All lanes: CDC42 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 22, 22 kDa Observed band size: 24 kDa

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC
• Clone: 3C3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CDC42 Antibody (3C3) - BSA Free Summary

• Description: Novus Biologicals Rabbit CDC42 Antibody (3C3) - BSA Free (NBP3-26670) is a recombinant monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human CDC42 [UniProt P60953]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDC42
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for CDC42 Antibody (3C3) - BSA Free

• CDC42
• CDC42Hs
• cell division control protein 42 homolog
• cell division cycle 42 (GTP binding protein, 25kDa)
• cell division cycle 42 (GTP-binding protein, 25kD)
• dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD))
• dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD))
• G25K GTP-binding protein
• G25K
• growth-regulating protein
• GTP-binding protein, 25kD
• small GTP binding protein CDC42

Background

CDC42 is a small GTPase of the Rho subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc42, and is able to complement the yeast cdc42 1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott Aldrich syndrome protein (NWASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in at least two transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC42