CDC25B Antibody

Product Discontinued

CDC25B Antibody Summary

• Immunogen: Recombinant fragment, corresponding to amino acids 140-185 of Human Cdc25B.
• Specificity: Cdc25B
• Clonality: Polyclonal
• Host: Chicken
• Gene: CDC25B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Electron Microscopy
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: ICC: Use at a dilution of 1/200. WB: Use at a dilution of 1/2000. Predicted molecular weight: 65 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
• Control:

  CDC25B Overexpression Lysate

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CDC25B Antibody

• CDC25B
• CDC25HU2
• cell division cycle 25 homolog B (S. cerevisiae)
• cell division cycle 25 homolog B (S. pombe)
• cell division cycle 25B
• Dual specificity phosphatase Cdc25B
• EC 3.1.3.48
• M-phase inducer phosphatase 2

Background

The activity of cyclin dependent kinases in cell cycle is regulated by the phosphorylation status, which is controlled by the antagonistic action of wee 1 kinase and Cdc25 phosphatases. Three Cdc25 genes are present in human cells: Cdc25A, Cdc25B and Cdc25C. Cdc25A and Cdc25B are expressed throughout the cell cycle, with peak expression in G1 for Cdc25A and in both G1 S phase and G2 for Cdc25B. Cdc25C is mainly expressed in G2 phase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC25B
• Entrez:
  • 994(Human)