CDC25B Antibody

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Western Blot: CDC25B Antibody [NB300-354] - Cdc25B Antibody [NB300-354] - E coli-derived fusion protein as test antigen. IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, EM, ICC/IF
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CDC25B Antibody Summary

Immunogen
Recombinant fragment, corresponding to amino acids 140-185 of Human Cdc25B.
Specificity
Cdc25B
Clonality
Polyclonal
Host
Chicken
Gene
CDC25B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Electron Microscopy
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
ICC: Use at a dilution of 1/200. WB: Use at a dilution of 1/2000. Predicted molecular weight: 65 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Positive Control
CDC25B Lysate (NBL1-08995)

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CDC25B Antibody

  • CDC25B
  • CDC25HU2
  • cell division cycle 25 homolog B (S. cerevisiae)
  • cell division cycle 25 homolog B (S. pombe)
  • cell division cycle 25B
  • Dual specificity phosphatase Cdc25B
  • EC 3.1.3.48
  • M-phase inducer phosphatase 2

Background

The activity of cyclin dependent kinases in cell cycle is regulated by the phosphorylation status, which is controlled by the antagonistic action of wee 1 kinase and Cdc25 phosphatases. Three Cdc25 genes are present in human cells: Cdc25A, Cdc25B and Cdc25C. Cdc25A and Cdc25B are expressed throughout the cell cycle, with peak expression in G1 for Cdc25A and in both G1 S phase and G2 for Cdc25B. Cdc25C is mainly expressed in G2 phase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CDC25B Antibody (NB300-354) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CDC25B
Entrez