CDC2/CDK1 [p Tyr15] Antibody

Images

 
Western Blot: Cdc2 [p Tyr15] Antibody [NB500-142] - Western Blot. Western blot of SK-N-MC total cell extracts, treated with hydroxyurea for 22 hours to induce Tyr15 cdc2 phosphorylation. The labeling by the antibody to ...read more

Product Details

Summary
Reactivity Hu, Rt, Xp, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

CDC2/CDK1 [p Tyr15] Antibody Summary

Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr15 conjugated to KLH. Accession # P39951
Modification
p Tyr15
Localization
Nuclear
Specificity
Specific for the ~38k cdc2 protein phosphorylated at Tyr15. Immunolabeling is blocked by the lambda-phosphatase treatment.
Predicted Species
Mouse (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Application Notes
NB500-142 can be used in Western blot where a band is seen at ~ 34 kDa, representing CDC2. Immunohistochemical analysis has not yet been determined.
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
CDC2/CDK1 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB500-142 in the following application:

Publications
Read Publications using
NB500-142 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Immunogen affinity purified

Alternate Names for CDC2/CDK1 [p Tyr15] Antibody

  • CDC2
  • CDC28A
  • CDC2MGC111195
  • CDK1
  • cell cycle controller CDC2
  • Cell division control protein 2 homolog
  • Cell division protein kinase 1
  • cyclin-dependent kinase 1
  • DKFZp686L20222
  • EC 2.7.11.22
  • EC 2.7.11.23
  • G1 to S and G2 to M
  • p34 protein kinase
  • P34CDC2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CDC2/CDK1 Antibody (NB500-142)(2)

We have publications tested in 1 confirmed species: Drosophila.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Showing Publications 1 - 2 of 2.
Publications using NB500-142 Applications Species
Forester CM, Maddox J, Louis JV et al. Control of mitotic exit by PP2A regulation of Cdc25C and Cdk1. Proc Natl Acad Sci U S A. 2007 Dec 11 [PMID: 18056802]
Wieschaus EF Temperature-induced uncoupling of cell cycle regulators BioRxiv Jan 1 2020 (ICC/IF, Drosophila) ICC/IF Drosophila

Review for CDC2/CDK1 Antibody (NB500-142) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB500-142:
Filter by Applications
Western Blot
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot CDC2/CDK1 NB500-142
Enlarge
5
reviewed by:
Lora Wilson
Western Blot Human 03/27/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
Lotcvp607

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FAQs for CDC2/CDK1 Antibody (NB500-142) (0)

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Control Lysate(s)

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Lora Wilson
03/27/2014
Application: Western Blot
Species: Human

Bioinformatics

Gene Symbol CDK1
Entrez
Uniprot