Reactivity | Hu, Rt, Xp, MuSpecies Glossary |
Applications | WB |
Clonality | Polyclonal |
Host | Rabbit |
Conjugate | Unconjugated |
Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr15 conjugated to KLH. Accession # P39951 |
Modification | p Tyr15 |
Localization | Nuclear |
Specificity | Specific for the ~38k cdc2 protein phosphorylated at Tyr15. Immunolabeling is blocked by the lambda-phosphatase treatment. |
Predicted Species | Mouse (100%). Backed by our 100% Guarantee. |
Isotype | IgG |
Clonality | Polyclonal |
Host | Rabbit |
Gene | CDK1 |
Purity | Immunogen affinity purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | NB500-142 can be used in Western blot where a band is seen at ~ 34 kDa, representing CDC2. Immunohistochemical analysis has not yet been determined. |
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Theoretical MW | 34 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Storage | Store at -20C. Avoid freeze-thaw cycles. |
Buffer | 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol |
Preservative | No Preservative |
Purity | Immunogen affinity purified |
Publications using NB500-142 | Applications | Species |
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Forester CM, Maddox J, Louis JV et al. Control of mitotic exit by PP2A regulation of Cdc25C and Cdk1. Proc Natl Acad Sci U S A. 2007 Dec 11 [PMID: 18056802] | ||
Wieschaus EF Temperature-induced uncoupling of cell cycle regulators BioRxiv Jan 1 2020 (ICC/IF, Drosophila) | ICC/IF | Drosophila |
Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Lora Wilson |
Western Blot | Human | 03/27/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
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