CD99 Antibody (MIC2/3478R) - Azide and BSA Free

Immunohistochemistry-Paraffin: CD99 Antibody (MIC2/3478R) - Azide and BSA Free [NBP3-08589] - Formalin-fixed, paraffin-embedded human Ewing's Sarcoma stained with CD99 Rabbit Recombinant Monoclonal Antibody (MIC2/3478R).

Immunohistochemistry-Paraffin: CD99 Antibody (MIC2/3478R) - Azide and BSA Free [NBP3-08589] - Formalin-fixed, paraffin-embedded human Ewing's Sarcoma stained with CD99 Rabbit Recombinant Monoclonal Antibody (MIC2/3478R).

Western blot analysis of MCF7 and A549 cell lysates using CD99 Antibody (MIC2/3478R) - Azide and BSA Free.

• Reactivity: Hu
• Applications: IHC
• Clone: MIC2/3478R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

CD99 Antibody (MIC2/3478R) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified by Protein A Column. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07315).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide corresponding to CD99 residues within aa85 C terminal of CD99 was used as an immunogen. (Uniprot: P14209 )
• Localization: Cell Surface
• Marker: Ewing s Sarcoma Marker
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CD99
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CD99 Antibody (MIC2/3478R) - Azide and BSA Free

• 12E7
• CD99 antigenY homolog
• CD99 molecule
• CD99
• E2 antigen
• HBA71
• MIC2 (monoclonal 12E7)
• MIC2
• MIC2Y
• MSK5X
• pilr-1
• PILR-L
• Protein MIC2
• surface antigen MIC2
• T-cell surface glycoprotein E2

Background

CD99 is encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD99