CD90/Thy1 Antibody (F7D5)

Flow Cytometry: CD90/Thy1 Antibody (F7D5) [NB100-64330] Staining of mouse thymus cells.

Product Discontinued

CD90/Thy1 Antibody (F7D5) Summary

• Immunogen: Monocyte derived dendritic cells
• Marker: Mesenchymal Cell Marker
• Specificity: NB100-64330 recognizes the mouse Thy1.2 alloantigen, expressed by thymocytes and peripheral T lymphocytes. Clone F7D5 reacts with Thy1.2 mice such as CBA and BALB/C, but not with Thy1.1 mice eg. AKR and FUB. The antibody is particularly useful for removal of T lymphocytes from cell populations by complement mediated cytotoxicity (1). This product is routinely tested in flow cytometry using mouse thymocytes.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: THY1
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:25
  • In vitro assay
• Application Notes: The antibody is particularly useful for removal of T lymphocytes from cell populations by complement mediated cytotoxicity

Reactivity Notes

Cross reacts with Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for CD90/Thy1 Antibody (F7D5)

• CD90 antigen
• CD90
• CDw90
• FLJ33325
• Thy-1 antigen
• Thy-1 cell surface antigen
• thy-1 membrane glycoprotein
• Thy-1 T-cell antigen
• Thy1

Background

CD90 (Thy1) antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for CD90/Thy1 Antibody (NB100-64330). (Showing 1 - 1 of 1 FAQ).

1. We are interested in CD90 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
   • I would recommend one of theses <a href="http://www.novusbio.com/productsearch/CD90%252FThy1#fq=common_name%3A%22CD90%2FThy1%22&fq=species%3A%22Canine%22" target="_self">CD90 antibodies</a>, as these have all been validated for use with dog/canine samples.

Bioinformatics

• Gene Symbol: THY1
• Entrez:
  • 21838(Mouse)
• Uniprot:
  • P01831()