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CD9 Antibody (P1/33/2) [DyLight 680]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC
Clone
P1/33/2
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 680

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-50154AF350
Alexa Fluor 405
NBP2-50154AF405
Alexa Fluor 488
NBP2-50154AF488
Alexa Fluor 532
NBP2-50154AF532
Alexa Fluor 594
NBP2-50154AF594
Alexa Fluor 647
NBP2-50154AF647
Alexa Fluor 700
NBP2-50154AF700
Alexa Fluor 750
NBP2-50154AF750
Allophycocyanin
NBP2-50154APC
Allophycocyanin/Cy7
NBP2-50154APCCY7
DyLight 350
NBP2-50154UV
DyLight 405
NBP2-50154V
DyLight 488
NBP2-50154G
DyLight 550
NBP2-50154R
DyLight 594
NBP2-50154DL594
DyLight 650
NBP2-50154C
DyLight 755
NBP2-50154IR
FITC
NBP2-50154F
Janelia Fluor 549
NBP2-50154JF549
Janelia Fluor 646
NBP2-50154JF646
PE
NBP2-50154PE
PE/Atto594
NBP2-50154PEATT594
PE/Cy5.5
NBP2-50154PECY55
PE/Cy7
NBP2-50154PECY7
PerCP
NBP2-50154PCP
Unconjugated
NBP2-50154
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CD9 Antibody (P1/33/2) [DyLight 680] Summary

Immunogen
Acute lymphoblastic leukaemia cells
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD9
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CD9 Antibody (P1/33/2) [DyLight 680]

  • BA2
  • BA-2/p24 antigen
  • BTCC-1
  • CD9 antigen
  • CD9 molecule
  • CD9
  • Cell growth-inhibiting gene 2 protein
  • DRAP-27
  • Leukocyte antigen MIC3
  • MIC3
  • motility related protein-1,5H9 antigen
  • Motility-related protein
  • MRP-1FLJ99568
  • p24
  • tetraspanin-29
  • TSPAN29
  • TSPAN-29
  • TSPAN29MIC3CD9 antigen (p24)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
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(SPV-L3)
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Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
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Applications: Flow, IHC, IHC-Fr, IF
CD81 Antibody (1D6)
NB100-65805
Western Blot: CD81 Antibody (1D6) [NB100-65805] - Isolation and characterization of exosomes from cell culture supernatant and human blood. Immunoblot of exosomal markers CD81 (~30 kDa), CD63 (~55 kDa), and LAMP2B (~50 kDa) in exosomes (5 ug lysates) isolated from the serum of breast cancer patients and healthy control. Grp94 (~100 kDa) and beta-actin (~42 kDa) serve as a negative control and a loading control, respectively. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep36502), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: CD81 Antibody (1D6) [NB100-65805] - The TAPA1 Antibody (1D6) was tested in A431 cells at a 1:10 dilution against DyLight 488 (green). Actin was counterstained against Phalloidin 568 (red) and cells were mounted in DAPI Flouromount (blue).
Immunohistochemistry-Paraffin: CD81 Antibody (1D6) [NB100-65805] - Staining of TAPA1 in human testis using DAB with hematoxylin counterstain.
(1 Review)
(9 Publications)
Species: Hu, Mu, Gt, Pm, Sh
Applications: WB, DB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
CD63 Antibody
(H5C6)
NBP2-42225
Western Blot: CD63 Antibody (H5C6) [NBP2-42225] - THP1 whole cell protein was separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was probed with anti-CD63 antibody at 2 ug/mL and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - The CD63 (H5C6) antibody was tested in HeLa cells at a 1:50 dilution against DyLight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.
Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - An intracellular stain was performed on SK-MEL-28 cells with CD63 (H5C6) Antibody NBP2-42225R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.
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(19 Publications)
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CD82/Kai-1
Antibody (C33)
NBP2-21792
Immunohistochemistry-Paraffin: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Staining of human tonsil (paraffin-embedded sections) with anti-CD82 (C33).
Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Analysis (surface staining) of CD82 on human peripheral blood cells with anti-CD82 (C33) PE.
Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Staining of CD82 on human peripheral blood cells with anti-CD82 (C33) APC.
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
MRP1 Antibody
(IU2H10)
NB400-156
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Immunocytochemistry/Immunofluorescence: MRP1 Antibody (IU2H10) [NB400-156] - The MRP-1 antibody was tested in HeLa cells at a 1:50 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.
Western Blot: MRP1 Antibody (IU2H10) [NB400-156] - Vector-transfected HEK293 cells 2: MRP1-transfected cells, prep1 3: MRP1-transfected cells, prep2 (the lower band is a degradation product.)
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Integrin beta
1/CD29 Antibody (P4C1
NBP2-36561
Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - Integrin beta 1 (P4C10) antibody was tested in -20 degree MeOH fixed HeLa cells at a 1:200 dilution against DyLight 488 (green). Actin and nuclei were counterstained against Phalloidin 568 (red) and DAPI (blue), respectively.
Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - An intracellular stain was performed on A431 cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).
Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561]
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CD151 Antibody
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Neprilysin/CD10
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1 µg/lane of Recombinant Human EGF was resolved with SDS-PAGE  and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.
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14.7)
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Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - An intracellular stain was performed on Raw264.7 cells with CD34 Antibody [MEC 14.7] NB600-1071 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 488 (SA5-10018, Thermo Fisher).
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Immunocytochemistry/Immunofluorescence: Fibronectin Antibody [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Fibronectin at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Analysis in a 6 month old Alport mouse kidney. Image from a verified customer review.
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CD19 Antibody
(CB19)
NBP2-25196
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Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - A surface stain was performed on Ramos cells with CD19 Antibody (CB19) NBP2-26646 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.
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ABCG2/CD338
Antibody (3G8)
NBP2-22124
Western Blot: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of ABCG2 expression in (1) human small intestine, (2) human placenta and (3) mouse placenta tissue extracts.
Immunocytochemistry/Immunofluorescence: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of Hela cells using ABCG2 mouse mAb (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).
Immunohistochemistry-Paraffin: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of paraffin-embedded bladder cancer tissues (left) and skeletal muscle tissues (right) using ABCG2 mouse mAb with DAB staining.
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CD53 Antibody
(MEM-53)
NB500-393
Flow Cytometry: CD53 Antibody (MEM-53) [NB500-393] - Staining of CD53 in human peripheral blood cells with anti-CD53 (MEM-53) PE.
Flow Cytometry: CD53 Antibody (MEM-53) [NB500-393] - Staining of CD53 in human peripheral blood cells with anti-CD53 (MEM-53) Pacific BlueTM.
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Integrin alpha
2b/CD41 Antibody
NBP1-84579
Immunohistochemistry-Paraffin: Integrin alpha 2b/CD41 Antibody [NBP1-84579] - Staining of human bone marrow.
Immunohistochemistry-Paraffin: Integrin alpha 2b/CD41 Antibody [NBP1-84579] - Staining of human bone marrow shows high expression.
Immunohistochemistry-Paraffin: Integrin alpha 2b/CD41 Antibody [NBP1-84579] - Staining of human stomach shows low expression as expected.
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Applications: IHC, IHC-P
ICAM-1/CD54
[Unconjugated]
796-IC
ICAM-1/CD54 [Unconjugated]
(1 Review)
(66 Publications)
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TNF-alpha
[Unconjugated]
210-TA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
(35 Reviews)
(649 Publications)
Species: Hu

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-50154AF350
Alexa Fluor 405 NBP2-50154AF405
Alexa Fluor 488 NBP2-50154AF488
Alexa Fluor 532 NBP2-50154AF532
Alexa Fluor 594 NBP2-50154AF594
Alexa Fluor 647 NBP2-50154AF647
Alexa Fluor 700 NBP2-50154AF700
Alexa Fluor 750 NBP2-50154AF750
Allophycocyanin NBP2-50154APC
Allophycocyanin/Cy7 NBP2-50154APCCY7
DyLight 350 NBP2-50154UV
DyLight 405 NBP2-50154V
DyLight 488 NBP2-50154G
DyLight 550 NBP2-50154R
DyLight 594 NBP2-50154DL594
DyLight 650 NBP2-50154C
DyLight 680 NBP2-50154FR
DyLight 755 NBP2-50154IR
FITC NBP2-50154F
Janelia Fluor 549 NBP2-50154JF549
Janelia Fluor 646 NBP2-50154JF646
PE NBP2-50154PE
PE/Atto594 NBP2-50154PEATT594
PE/Cy5.5 NBP2-50154PECY55
PE/Cy7 NBP2-50154PECY7
PerCP NBP2-50154PCP
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Bioinformatics

Gene Symbol CD9