CD81 Antibody

Images

 
Western Blot: CD81 Antibody [NBP2-20564] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: CD81 Antibody [NBP2-20564] - MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD81 protein stained by CD81 antibody diluted at 1:500. Blue: Hoechst 33342 ...read more
Immunohistochemistry-Paraffin: CD81 Antibody [NBP2-20564] - CD81 Antibody Immunohistochemical analysis of paraffin-embedded Lung Papillary Adenocarcinoma, using antibody at 1:250 dilution.
Flow Cytometry: CD81 Antibody [NBP2-20564] - THP-1 cell. Black: Unlabelled sample was used as a control. Red: CD81 antibody dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated ...read more

Product Details

Summary
Reactivity Hu, Po, Mk, Pm, ShSpecies Glossary
Applications WB, B/N, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.08 mg/ml

Order Details

CD81 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human CD81. The exact sequence is proprietary.
Localization
Membrane
Predicted Species
Porcine (92%), Sheep (92%), Monkey (96%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CD81
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Blocking/Neutralizing 50 - 200 molar excess
  • Flow Cytometry 1:50
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rhesus Monkey (96%), Chimpanzee (100%), Mouse (83%), Rat (86%), Bovine (89%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
1.08 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CD81 Antibody

  • CD81 antigen (target of antiproliferative 1)
  • CD81 antigen
  • CD81 molecule
  • CD81
  • CVID6
  • TAPA1
  • TAPA-1
  • Target of the antiproliferative 1
  • Tetraspanin-28
  • TSPAN28
  • Tspan-28
  • tspan-28,26 kDa cell surface protein TAPA-1
  • TSPAN28S5.7

Background

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD81 Antibody (NBP2-20564) (0)

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Secondary Antibodies

 

Isotype Controls

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Tools for Isolation, Quantification and Analysis of Exosomes
Exosomes are spherical to cup-shaped bilayered membrane enclosed nanosize vesicles (30-100 nm) which have the ability to shuttle active cargoes between cells. Johnstone et al. 1987 pioneered in documenting the generation of exosomes in differentiat...  Read full blog post.

CD19: An Undoubted Biomarker for B Cells
CD19 is a cell surface protein member of the large immunoglobulin superfamily that complexes with CD21, CD81, and CD225 in the membrane of mature B-cells. A major function of CD19 is to assemble with the antigen receptor of B-lymphocytes to decrease t...  Read full blog post.

CD81/TAPA1: I'm on Tapa the Cell
Target of the antiproliferative 1 (TAPA1), also known as CD81, is found in the plasma membrane in lymphocytes and plays an important role in the regulation of lymphoma cell growth. This transmembrane 4 superfamily (TM4SF) protein is primarily found on...  Read full blog post.

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Bioinformatics

Gene Symbol CD81
Entrez
Uniprot