CD81 Antibody (SN206-01)

Western Blot: CD81 Antibody (SN206-01) [NBP2-67722] - Analysis of TAPA1/CD81 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: PC-12 cell lysateLane 2: JAR cell lysate

Immunocytochemistry/Immunofluorescence: CD81 Antibody (SN206-01) [NBP2-67722] - Staining CD81 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CD81 Antibody (SN206-01) [NBP2-67722] - Analysis of paraffin-embedded mouse testis tissue using anti-CD81 antibody. Counter stained with hematoxylin.

Flow Cytometry: CD81 Antibody (SN206-01) [NBP2-67722] - Analysis of Jurkat cells with CD81 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Immunocytochemistry/Immunofluorescence: CD81 Antibody (SN206-01) [NBP2-67722] - Staining CD81 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: CD81 Antibody (SN206-01) [NBP2-67722] - Staining CD81 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: CD81 Antibody (SN206-01) [NBP2-67722] - Staining CD81 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CD81 Antibody (SN206-01) [NBP2-67722] - Analysis of paraffin-embedded human liver tissue using anti-CD81 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CD81 Antibody (SN206-01) [NBP2-67722] - Analysis of paraffin-embedded mouse lung tissue using anti-CD81 antibody. Counter stained with hematoxylin.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: SN206-01
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

CD81 Antibody (SN206-01) Summary

• Description: Novus Biologicals Rabbit CD81 Antibody (SN206-01) (NBP2-67722) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-CD81 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human CD81 aa 161-210 / 236. (SwissProt: P60033 Human; SwissProt: P35762 Mouse; SwissProt: Q62745 Rat)
• Localization: Membrane.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CD81
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CD81 Antibody (SN206-01)

• CD81 antigen (target of antiproliferative 1)
• CD81 antigen
• CD81 molecule
• CD81
• CVID6
• TAPA1
• TAPA-1
• Target of the antiproliferative 1
• Tetraspanin-28
• TSPAN28
• Tspan-28
• tspan-28,26 kDa cell surface protein TAPA-1
• TSPAN28S5.7

Background

CD81/TAPA-1 is an integral membrane protein expressed on a variety of cell types, and has a high degree of sequence homology between human and mouse. (1) CD81 is expressed on thymic stromal cells where it plays an important role in the transition of gamma delta + T cells to more mature T cells with alpha beta T cell receptors. (2) Immunohistochemical staining has revealed that its expression is localized to the subcapsular region of the thymus and, specifically, on cells that have distinct clustering patterns. It has been speculated that the ligand for CD81 is the pre-T cell receptor, which is composed of a TCR beta chain and glycoprotein pT alpha. (2) The monoclonal antibody 2F7 can block thymocyte interaction with CD81 in vitro. (2)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD81 Antibody (NBP2-67722)(3)

Publications using NBP2-67722

• Ismaeel A, Van Pelt DW, Hettinger ZR et al. Extracellular vesicle distribution and localization in skeletal muscle at rest and following disuse atrophy Skeletal muscle 2023-03-10 [PMID: 36895061] (Immunohistochemistry-Frozen, Rat)
• Xie YX, Naseri NN, Fels J et al. Lysosomal exocytosis releases pathogenic alpha-synuclein species from neurons in synucleinopathy models Nature communications 2022-08-22 [PMID: 35995799] (WB, Mouse)
• Sadri M, Hirosawa N, Le J et al. Tumor necrosis factor receptor-1 is selectively sequestered into Schwann cell extracellular vesicles where it functions as a TNF alpha decoy Glia 2021-09-24 [PMID: 34559433]

Review for CD81 Antibody (NBP2-67722) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Adam Bennett WB Mouse 02/28/2024

Summary

• Application: Western Blot
• Sample Tested: Brain
• Species: Mouse
• Lot: H01008

Comments

• Comments: Used at 1:500 in 5% BSA in TBST overnight at 4C.
  Used with 7.5 ug mouse brain-derived extracellular vesicles.
  Exposed with West Femto ECL

Bioinformatics

• Gene Symbol: CD81