CD81 Antibody (M38) [FITC]

• Reactivity: Hu, Mu, Fe, Rb
• Applications: WB, Flow, ICC/IF, IHC, IP, KO
• Clone: M38
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

CD81 Antibody (M38) [FITC] Summary

• Description: Store in the dark. Avoid prolonged exposure to light.
• Immunogen: MOLT-4, human T-ALL cell line (NM_004356.3).
• Specificity: The antibody M38 reacts with CD81, a 25 kDa member of the tetraspanin family, expressed on majority of cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD81
• Purity: Size Exclusion Chromatography

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Knockout Validated
  • Western Blot 1:100-1:2000
• Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension.
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cat (100%). Use in Mouse reported in scientific publication( PMID: 32573489).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Purity: Size Exclusion Chromatography

Alternate Names for CD81 Antibody (M38) [FITC]

• CD81 antigen (target of antiproliferative 1)
• CD81 antigen
• CD81 molecule
• CD81
• CVID6
• TAPA1
• TAPA-1
• Target of the antiproliferative 1
• Tetraspanin-28
• TSPAN28
• Tspan-28
• tspan-28,26 kDa cell surface protein TAPA-1
• TSPAN28S5.7

Background

CD81 (TAPA-1), a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T and other cells R11; e.g. in muscles CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD81 Antibody (NBP1-44859)(2)

Publications using NBP1-44859

• Sung SE, Seo MS, Kang KK et al. Mesenchymal Stem Cell Exosomes Derived from Feline Adipose Tissue Enhance the Effects of Anti-Inflammation Compared to Fibroblasts-Derived Exosomes Vet Sci 2021-09-03 [PMID: 34564576] (FLOW, Feline)
  
  Details:
  Citation using the FITC format of this antibody.
• Sung SE, Seo MS, Kang KK et al. Isolation and Characterization of Extracellular Vesicle from Mesenchymal Stem Cells of the Epidural Fat of the Spine Asian Spine Journal 2022-04-30 [PMID: 34461688] (Flow Cytometry)

Bioinformatics

• Gene Symbol: CD81