CD7 Antibody (B-F12)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, ICC/IF
Clone
B-F12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

CD7 Antibody (B-F12) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.
Additional Information
Clone B-F12 was used by HLDA to establish CD designation.
Immunogen
Chronic Lymphoid Leukemia cells (CLL)
Localization
Cell Surface
Marker
T-Cell Leukemia Marker
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD7
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5-1 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 0.5-1 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Others not known.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for CD7 Antibody (B-F12)

  • CD7 antigen (p41)
  • CD7 antigen
  • CD7 molecule
  • CD7
  • GP40T-cell surface antigen Leu-9
  • LEU-9
  • T-cell antigen CD7
  • T-cell leukemia antigen
  • Tp40
  • TP41
  • TP41p41 protein

Background

CD7, also known as gp40, is a 40 kD type I transmembrane glycoprotein member of the immunoglobulin superfamily. It is found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL). CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 co-stimulation can induce cytokine secretion and modulate cellular adhesion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for CD7 Antibody (NBP2-44799) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD7
Uniprot