CD69 Antibody (15B5G2) - Azide Free

Images

 
Western Blot: CD69 Antibody (15B5G2) - Azide Free [NBP2-25242] - analysis of CD69 in A) a partial recombinant protein and B) ThP1 lysate using CD69 antibody at 0.5 ug/ml. goat anti-mouse Ig HRP secondary antibody and ...read more
Immunohistochemistry-Paraffin: CD69 Antibody (15B5G2) - Azide Free [NBP2-25242] - Formalin-fixed, paraffin-embedded human tonsil stained with CD69 antibody (1 ug/ml), peroxidase-conjugate and DAB chromogen.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clone
15B5G2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide Free

CD69 Antibody (15B5G2) - Azide Free Summary

Immunogen
Amino acids 103-199 of human CD69 were used as the immunogen for the antibody.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD69
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-5 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1-3 ug/ml

Reactivity Notes

This product reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml sterile PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for CD69 Antibody (15B5G2) - Azide Free

  • activation inducer molecule (AIM/CD69)
  • Activation inducer molecule
  • AIM
  • BL-AC/P26
  • CD69 antigen (p60, early T-cell activation antigen)
  • CD69 antigen
  • CD69 molecule
  • CD69
  • CLEC2CC-type lectin domain family 2 member C
  • C-type lectin domain family 2, member C
  • EA1
  • EA-1
  • early activation antigen CD69
  • early lymphocyte activation antigen
  • Early T-cell activation antigen p60
  • GP32/28
  • Leu23
  • Leukocyte surface antigen Leu-23
  • MLR-3
  • p60

Background

CD69 is a transmembrane C-type lectin broadly expressed on the cell surface of activated leukocytes, including T and B lymphocytes, neutrophils, natural killer cells, and eosinophils, and is constitutively expressed on the surface of monocytes and platelets (Vance BA et al., 2005 15893733). The abundance of CD69 rapidly increases after cell activation in all bone marrow-derived cells except erythrocytes (Martin P et al., 2011 21427408). It is an earliest lymphocyte activation antigen and a universal leukocyte triggering molecule expressed at sites of active immune response (Kavan D et al., 2010 20369839). CD69 plays a key role in early inflammation and modulation of adaptive immune responses through the production of a variety of cytokines and lymphocyte migration. Expression of CD69 has been observed in human diseases. Moreover anti-CD69 monoclonal antibodies have therapeutic effect in the treatment process of different animal models of cancer and autoimmune disorders (Vazquez BN et al., 2012 22456278). Surface-expressed CD69 is an activation marker for basophils and is strongly induced by IL-3. Upregulated CD69 expression on locally accumulated basophils in bronchial asthma attributed at least in part to a combination of local cytokines, especially IL-3, plus exposure to low levels of IgE-crosslinking allergens (Suzukawa M et al., 2007 17541278).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-25242AF405
Alexa Fluor 488 Labeled NBP2-25242AF488
Alexa Fluor 647 Labeled NBP2-25242AF647
Alexa Fluor 700 Labeled NBP2-25242AF700
Biotin Labeled NBP2-25242B
DyLight 350 Labeled NBP2-25242UV
DyLight 405 Labeled NBP2-25242V
DyLight 488 Labeled NBP2-25242G
DyLight 550 Labeled NBP2-25242R
DyLight 650 Labeled NBP2-25242C
DyLight 680 Labeled NBP2-25242FR
DyLight 755 Labeled NBP2-25242IR
HRP Labeled NBP2-25242H

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Bioinformatics

Gene Symbol CD69
Entrez