CD63 Antibody (SPM524) - Azide and BSA Free

Images

 
Western Blot: CD63 Antibody (SPM524) - Azide and BSA Free [NBP2-34779] - Western blot of human spleen Lysate with CD63 Monoclonal Antibody (SPM524)
Immunohistochemistry-Paraffin: CD63 Antibody (SPM524) - Azide and BSA Free [NBP2-34779] - Formalin-fixed paraffin-embedded human melanoma stained with CD63 monoclonal antibody (SPM524) (AEC chromogen)
Immunohistochemistry-Paraffin: CD63 Antibody (SPM524) [Azide Free] [NBP2-34779] - Formalin-paraffin mouse spleen stained with CD63 MAb (SPM524)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC-Fr, IHC-P, IP
Clone
SPM524
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

CD63 Antibody (SPM524) - Azide and BSA Free Summary

Immunogen
Recombinant human CD63 protein
Marker
Late Endosomes Marker
Specificity
This product is specific for CD63
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD63
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • ELISA 1-5ug/ml for coating
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry-Frozen 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 0.5-1ug/500ug protein lysate
Application Notes
Immunohistochemistry (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Reactivity Notes

This product is specific for Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS - BSA free
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for CD63 Antibody (SPM524) - Azide and BSA Free

  • CD63 antigen (melanoma 1 antigen)
  • CD63 antigen
  • CD63 molecule
  • CD63
  • Granulophysin
  • Lamp-3
  • Lysosomal-associated membrane protein 3
  • ME491
  • melanoma 1 antigen
  • Melanoma-associated antigen ME491
  • melanoma-associated antigen MLA1
  • MLA1lysosome-associated membrane glycoprotein 3
  • Ocular melanoma-associated antigen
  • OMA81H
  • tetraspanin-30
  • Tspan30
  • tspan-30
  • TSPAN30granulophysin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD63 Antibody (NBP2-34779) (0)

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Secondary Antibodies

 

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Discover related pathways, diseases and genes to CD63 Antibody (NBP2-34779). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on CD63.

Tools for Isolation, Quantification and Analysis of Exosomes
Exosomes are spherical to cup-shaped bilayered membrane enclosed nanosize vesicles (30-100 nm) which have the ability to shuttle active cargoes between cells. Johnstone et al. 1987 pioneered in documenting the generation of exosomes in differentiat...  Read full blog post.

CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

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Bioinformatics

Gene Symbol CD63