CD55/DAF Antibody (028) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: CD55/DAF Antibody (028) [NBP2-89269] - Staining of Human CD55 in JURKAT cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human CD55 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.

Flow Cytometry: CD55/DAF Antibody (028) [NBP2-89269] - Analysis of human CD55 expression on K562 cells. Cells were stained with purified anti-Human CD55, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, ELISA
• Clone: 028
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CD55/DAF Antibody (028) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Human CD55/DAF (Accession#: NP_000565.1; Met 1-Ser 353).
• Specificity: No cross-reactivity in ELISA with:
  
  Human CD97
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CD55
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Flow Cytometry 1:25-1:100
  • Immunocytochemistry/ Immunofluorescence 1:10-1:40
  • Sandwich ELISA Capture 1:250-1:2000
• Application Notes: This antibody will detect Human CD55/DAF in ELISA pair set (Catalog: # NBP2-79440).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for CD55/DAF Antibody (028) - Azide and BSA Free

• CD55 antigen
• CD55 molecule, decay accelerating factor for complement (Cromer blood group)
• CD55
• CR
• CRdecay accelerating factor for complement (CD55, Cromer blood group system)
• CROMDAFcomplement decay-accelerating factor
• DAF
• decay accelerating factor for complement
• TC

Background

CD55 encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD55