CD59 Antibody (MEM-43)

Images

 
Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) Pacific BlueTM.
Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Analysis using the PE conjugate of NB500-330. Surface staining of human peripheral blood cells with anti-human CD59 (MEM-43) PE.
Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Using the PE direct conjugate Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) PE.
Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Using the Allophycocyanin direct conjugate Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) APC.
Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) FITC.
Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) biotin / streptavidin-APC.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications B/N, Flow, IHC, IHC-P, IP, CyTOF-ready
Clone
MEM-43
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

CD59 Antibody (MEM-43) Summary

Immunogen
Thymocytes and T lymphocytes (NM_000611.5).
Specificity
The antibody MEM-43 reacts with well defined epitope (W40, R-53) on CD59 (Protectin), an 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissues. HLDA IV; WS Code NL 705 HLDA V; WS Code AS S013 HLDA V; WS Code BP BP345 HLDA V; WS Code T T-103
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CD59
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Blocking/Neutralizing
  • Flow Cytometry 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Immunoprecipitation 1:50
  • CyTOF-ready
Application Notes
Purity is > 95% (by SDS-PAGE). Use in blocking / neutralizing reported in scientific literature (PMID 24909955)
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
CD59 Lysate (NBL1-08958)
Publications
Read Publications using
NB500-330 in the following applications:

  • B/N
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for CD59 Antibody (MEM-43)

  • 16.3A5
  • 1F5 antigen
  • 1F5
  • 20 kDa homologous restriction factor
  • CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16
  • CD59 antigen
  • CD59 antigen, complement regulatory protein
  • CD59 glycoprotein
  • CD59 molecule, complement regulatory protein
  • CD59
  • EJ16
  • EJ30
  • EJ30, EL32 and G344)
  • EL32
  • FLJ38134
  • FLJ92039
  • G344
  • HRF20
  • HRF-20
  • human leukocyte antigen MIC11
  • Ly-6-like protein
  • lymphocytic antigen CD59/MEM43
  • MACIF
  • MAC-inhibitory protein
  • MAC-IP
  • MEM43 antigen
  • MEM43
  • membrane attack complex (MAC) inhibition factor
  • Membrane attack complex inhibition factor
  • Membrane inhibitor of reactive lysis
  • MGC2354
  • MIC11
  • MIC11MSK21
  • MIN1
  • MIN2
  • MIN3
  • MIRL
  • p18-20
  • Protectin
  • surface anitgen recognized by monoclonal 16.3A5
  • T cell-activating protein

Background

CD59 (Protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD59 Antibody (NB500-330)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: B/N.


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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol CD59
Entrez