CD59 Antibody (MEM-43)

Images

 
Immunohistochemistry-Paraffin: CD59 Antibody (MEM-43) [NB500-330] - Staining of human placenta (paraffin sections) using anti-CD59 (MEM-43).
Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) Pacific BlueTM.
Immunohistochemistry-Frozen: CD59 Antibody (MEM-43) [NB500-330] - Human tonsil frozen tissue section stained for CD59 (red) and counterstained with DAPI (blue). Image from verified customer review.
Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Analysis using the PE conjugate of NB500-330. Surface staining of human peripheral blood cells with anti-human CD59 (MEM-43) PE.
Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Using the PE direct conjugate Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) PE.
Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Using the Allophycocyanin direct conjugate Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) APC.
Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) FITC.
Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) biotin / streptavidin-APC.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, IHC-Fr, IHC-P, IP, B/N, CyTOF-ready
Clone
MEM-43
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CD59 Antibody (MEM-43) Summary

Immunogen
Thymocytes and T lymphocytes (NM_000611.5).
Specificity
The antibody MEM-43 reacts with well defined epitope (W40, R-53) on CD59 (Protectin), an 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissues.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CD59
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 10 ug/mL
  • Immunoprecipitation 1:50
  • Block/Neutralize
  • CyTOF-ready
Application Notes
Use in blocking / neutralizing reported in scientific literature (PMID 24909955). CD59 antibody validated for IHC-F from a verified customer review. This antibody is CyTOF ready.
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
CD59 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB500-330 in the following applications:

Publications
Read Publications using
NB500-330 in the following applications:

  • B/N
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for CD59 Antibody (MEM-43)

  • 16.3A5
  • 1F5 antigen
  • 1F5
  • 20 kDa homologous restriction factor
  • CD59 antigen
  • CD59 antigen, complement regulatory protein
  • CD59 glycoprotein
  • CD59 molecule, complement regulatory protein
  • CD59
  • EJ16
  • EJ30
  • EJ30, EL32 and G344)
  • EL32
  • FLJ38134
  • FLJ92039
  • G344
  • HRF20
  • HRF-20
  • human leukocyte antigen MIC11
  • Ly-6-like protein
  • lymphocytic antigen CD59/MEM43
  • MACIF
  • MAC-inhibitory protein
  • MAC-IP
  • MEM43 antigen
  • MEM43
  • membrane attack complex (MAC) inhibition factor
  • Membrane attack complex inhibition factor
  • Membrane inhibitor of reactive lysis
  • MGC2354
  • MIC11
  • MIC11MSK21
  • MIN1
  • MIN2
  • MIN3
  • MIRL
  • p18-20
  • Protectin
  • surface anitgen recognized by monoclonal 16.3A5
  • T cell-activating protein

Background

CD59 (Protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD59 Antibody (NB500-330)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: B/N.


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B/N
(1)
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Human
(1)
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Review for CD59 Antibody (NB500-330) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB500-330:
Filter by Applications
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 CD59 NB500-330
Enlarge
reviewed by:
Jan Martinek
Human 11/15/2018
View

Summary

Sample TestedHuman Tonsil tissue
SpeciesHuman
Lot529791

Comments

CommentsAcetone fixation

Product General Protocols

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FAQs for CD59 Antibody (NB500-330) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB500-330AF350
Alexa Fluor 405 NB500-330AF405
Alexa Fluor 488 NB500-330AF488
Alexa Fluor 532 NB500-330AF532
Alexa Fluor 594 NB500-330AF594
Alexa Fluor 647 NB500-330AF647
Alexa Fluor 700 NB500-330AF700
Alexa Fluor 750 NB500-330AF750
Allophycocyanin NB500-330APC
Allophycocyanin/Cy7 NB500-330APCCY7
Biotin NB500-330B
DyLight 350 NB500-330UV
DyLight 405 NB500-330V
DyLight 488 NB500-330G
DyLight 550 NB500-330R
DyLight 594 NB500-330DL594
DyLight 650 NB500-330C
DyLight 680 NB500-330FR
DyLight 755 NB500-330IR
FITC NB500-330F
HRP NB500-330H
Janelia Fluor 549 NB500-330JF549
Janelia Fluor 646 NB500-330JF646
PE NB500-330PE
PE/Atto594 NB500-330PEATT594
PE/Cy5.5 NB500-330PECY55
PE/Cy7 NB500-330PECY7
PerCP NB500-330PCP

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Recent Reviews

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5
0
4
1
3
0
2
0
1
0

Jan Martinek
11/15/2018
Application:
Species: Human

Bioinformatics

Gene Symbol CD59