CD53 Antibody (HD77) - Chimeric - Azide and BSA Free

Western Blot: CD53 Antibody (HD77) - Chimeric [NBP3-12059] - Human lymph node (A), human thymus (B) and human spleen (C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HD77 (NBP3-12059) at 0.1 ug/ml, 0.3 ug/ml and 0.5 ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunocytochemistry /Immunofluorescence: CD53 Antibody (HD77) - Chimeric - Azide and BSA Free [NBP3-12059] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with NBP3-12059 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12059, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Flow Cytometry: CD53 Antibody (HD77) - Chimeric - Azide and BSA Free [NBP3-12059] - Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12059 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: HD77
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

CD53 Antibody (HD77) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit CD53 Antibody (HD77) - Chimeric - Azide and BSA Free (NBP3-12059) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was raised by immunising mice with human leukemia cells.
• Specificity: This antibody is specific for the extracytoplasmic part of human CD53, a pan-leukocyte antigen.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CD53
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CD53 Antibody (HD77) - Chimeric - Azide and BSA Free

• antigen MOX44 identified by monoclonal MRC-OX44
• CD53 antigentetraspanin-25
• CD53 glycoprotein
• CD53 molecule
• CD53 tetraspan antigen
• CD53
• cell surface antigen
• Cell surface glycoprotein CD53
• MOX44
• MOX44transmembrane glycoprotein
• Tetraspanin-25
• TSPAN25
• tspan-25
• TSPAN25leukocyte surface antigen CD53

Background

The antibody reacts with CD53 antigen, a glycoprotein of 32-40 kDa belonging to the tetraspans family (TM4SF). CD53 antigen is exclusivelly expressed on leucocytes (not present on platelets, red blood cells and non-hematopoietic cells). CD53 is important for signal transduction. CD53 cross-linking promotes activation of human B cells and rat macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD53