CD5 Antibody (4H8E5) [Allophycocyanin]

Flow Cytometry: CD5 Antibody (4H8E5) [Allophycocyanin] [NBP1-26707] - BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD5-FITC (NBP1-26704) and Rat Anti-Mouse CD19-PE (NBP1-27894).

• Reactivity: Mu
• Applications: Flow
• Clone: 4H8E5
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Allophycocyanin
• Concentration: 0.1 mg/ml

CD5 Antibody (4H8E5) [Allophycocyanin] Summary

• Immunogen: The immunogen is a peptide made to the amino acid region CD5
• Specificity: CD5/Lyt-1, Mr 67 kDa
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: CD5
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry <= 0.2 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS and a stabilizing agent
• Preservative: Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for CD5 Antibody (4H8E5) [Allophycocyanin]

• CD5 antigen (p56-62)
• CD5 antigen
• CD5 molecule
• CD5
• LEU1T-cell surface glycoprotein CD5
• Lymphocyte antigen T1/Leu-1
• T1

Background

CD5/Lyt-1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. (1-3) It has been identified as the major ligand of the B-cell antigen CD72.4, 5 The frequency of CD5+ B cells exhibits strain-dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells (reviewed in 6). Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression. (7-12) It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B-cell receptors. (13, 14)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD5