CD5 Antibody (4H8E5) [FITC]

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Flow Cytometry: CD5 Antibody (4H8E6) [FITC] [NBP1-26704] - Analysis of C57BL/6 splenocytes by multiple staining.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr, IP
Clone
4H8E5
Clonality
Monoclonal
Host
Rat
Conjugate
FITC

Order Details

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CD5 Antibody (4H8E5) [FITC] Summary

Immunogen
The immunogen is a peptide made to the amino acid region CD5
Specificity
CD5/Lyt-1, Mr 67 kDa
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
CD5
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry <= 1 ug/10^6 cells
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for CD5 Antibody (4H8E5) [FITC]

  • CD5 antigen (p56-62)
  • CD5 antigen
  • CD5 molecule
  • CD5
  • LEU1T-cell surface glycoprotein CD5
  • Lymphocyte antigen T1/Leu-1
  • T1

Background

CD5/Lyt-1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. (1-3) It has been identified as the major ligand of the B-cell antigen CD72.4, 5 The frequency of CD5+ B cells exhibits strain-dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells (reviewed in 6). Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression. (7-12) It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B-cell receptors. (13, 14)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NBP1-26707
Biotin Labeled NBP1-26705
PE Labeled NBP1-26706

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Bioinformatics

Gene Symbol CD5
Entrez