CD5 Antibody (4H8E5) [PE]

Flow Cytometry: CD5 Antibody (4H8E6) [PE] [NBP1-26706] - 0.2 ug/1^6 cells from BALB/c spleen were double-stained with rat anti-mouse CD5-R-PE and rat anti-mouse CD19-FITC Clone 6D5), following which small lymphocytes were gated and analyzed using a flow cytometer

• Reactivity: Mu
• Applications: WB, Flow, IHC, IP
• Clone: 4H8E5
• Clonality: Monoclonal
• Host: Rat
• Conjugate: PE
• Concentration: 0.1 mg/ml

CD5 Antibody (4H8E5) [PE] Summary

• Immunogen: The immunogen is a peptide made to the amino acid region CD5
• Specificity: CD5/Lyt-1, Mr 67 kDa
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: CD5
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry <= 0.2 ug/10^6 cells
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS and a stabilizing agent
• Preservative: Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for CD5 Antibody (4H8E5) [PE]

• CD5 antigen (p56-62)
• CD5 antigen
• CD5 molecule
• CD5
• LEU1T-cell surface glycoprotein CD5
• Lymphocyte antigen T1/Leu-1
• T1

Background

CD5/Lyt-1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. (1-3) It has been identified as the major ligand of the B-cell antigen CD72.4, 5 The frequency of CD5+ B cells exhibits strain-dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells (reviewed in 6). Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression. (7-12) It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B-cell receptors. (13, 14)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD5 Antibody (NBP1-26706)(1)

Publication using NBP1-26706

• Vasilevska J, Cheng PF, Lehmann J, Ramelyte E, G�mez JM, Dimitriou F, Sella F; TuPro Consortium; Ferretti D, Salas-Bastos A, Jordaan WS, Levesque MP, Dummer R, Sommer L. Monitoring melanoma patients on treatment reveals a distinct macrophage population driving targeted therapy resistance Cell Rep Med 2024-06-27 [PMID: 38942020]

Bioinformatics

• Gene Symbol: CD5