CD5 Antibody (4H8E5) - Azide and BSA Free

Flow Cytometry: CD5 Antibody (4H8E5) [NBP1-26703] - Analysis using the PE conjugate of NBP1-26703. Double staining of 0.2 ug/1^6 cells from BALB/c spleen with rat anti-mouse CD5-R-PE and rat anti-mouse CD19-FITC Clone 6D5), following which small lymphocytes were gated and analyzed using a flow cytometer.

Flow Cytometry: CD5 Antibody (4H8E5) [NBP1-26703] - Analysis using the Allophycocyanin conjugate of NBP1-26703. Multiple staining of C57BL/6 splenocytes.

Flow Cytometry: CD5 Antibody (4H8E5) [NBP1-26703] - Analysis using the Biotin conjugate of NBP1-26703. Multiple staining of C57BL/6 splenocytes.

Flow Cytometry: CD5 Antibody (4H8E5) [NBP1-26703] - Analysis using the FITC conjugate of NBP1-26703. Multiple staining of C57BL/6 splenocytes.

• Reactivity: Mu
• Applications: Flow
• Clone: 4H8E5
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

CD5 Antibody (4H8E5) - Azide and BSA Free Summary

• Description: Novus Biologicals Rat CD5 Antibody (4H8E5) - Azide and BSA Free (NBP1-26703) is a monoclonal antibody validated for use in Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen is a peptide made to the amino acid region CD5
• Specificity: CD5/Lyt-1, Mr 67 kDa
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: CD5
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1 ug/10 cells

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.1M BBS (pH 8.2)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD5 Antibody (4H8E5) - Azide and BSA Free

• CD5 antigen (p56-62)
• CD5 antigen
• CD5 molecule
• CD5
• LEU1T-cell surface glycoprotein CD5
• Lymphocyte antigen T1/Leu-1
• T1

Background

CD5/Lyt-1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. (1-3) It has been identified as the major ligand of the B-cell antigen CD72.4, 5 The frequency of CD5+ B cells exhibits strain-dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells (reviewed in 6). Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression. (7-12) It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B-cell receptors. (13, 14)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD5