CD47 Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, Flow, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CD47 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CD47 N-terminal IgV-like Extracellular Domain
  Gln19-Pro158
  Accession # NP_034711
• Specificity: Detects mouse CD47 N-terminal IgV-like Extracellular Domain in Western blots. In Western blots, approximately 15% cross‑reactivity with recombinant human CD47 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CD47
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD47 Antibody [Biotin]

• antigen identified by monoclonal 1D8
• Antigenic surface determinant protein OA3
• CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
• CD47 antigen
• CD47 glycoprotein
• CD47 molecule
• CD47
• IAP
• IAPintegrin associated protein
• Integrin-associated protein
• leukocyte surface antigen CD47
• MER6
• MER6integrin-associated signal transducer
• OA3
• Protein MER6
• Rh-related antigen

Background

CD47, also known as integrin-associated protein, is an integral membrane protein with a single IgV-like domain at its N-terminus followed by five membrane-spanning regions. CD47 is expressed ubiquitously. It is a ligand for the transmembrane signal regulatory protein alpha 1 (SIRP alpha ) and also functions as a receptor for thrombospondin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD47 Antibody (BAF1866)(1)

Publication using BAF1866

• Y He, X Sun, W Rong, R Yang, H Liang, Y Qi, L Li, K Zen CD47 is a negative regulator of intestinal epithelial cell self-renewal following DSS-induced experimental colitis Sci Rep, 2020-06-23;10(1):10180. 2020-06-23 [PMID: 32576895] (IHC, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: CD47
• Uniprot:
  • NP_034711()