Livin Antibody (88C570) - BSA Free

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Western Blot: Livin Antibody (88C570) [NB100-56548] - detection of Livin in A) Livin transfected cell lysate and B) Molt4 cell lysate. A protein band of apprximate molecular weight of 31 kDa is detected using this ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
88C570
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Livin Antibody (88C570) - BSA Free Summary

Immunogen
Recombinant human livin protein was used as immunogen.
Specificity
The Livin antibody, clone 88C570 has been shown to recognize both Livin alpha (~39 kDa) and Livin beta (~37 kDa) isoforms (Nachmias et al, 2003). 2. The antibody also recognizes the 30 kDa (p30-Livin alpha) and 28 kDa (p28-Livin beta) apoptotic cleavage fragments of Livin alpha and beta, respectively (Nachmias et al, 2003). Apoptotic cleavage fragments of Livin have also been referred to as truncated livin (tLivin) (Nachmias et al, 2007). 3. The specificity of the antibody has been validated using Livin shRNA (Quiping et al, 2004).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BIRC7
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-5 ug/ml. Use reported in scientific literature (Nachmias et al (2007))
  • Western Blot 1-2 ug/ml
Publications
Read Publications using
NB100-56548 in the following applications:

  • 1 publication
  • WB
    16 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Livin Antibody (88C570) - BSA Free

  • baculoviral IAP repeat containing 7
  • baculoviral IAP repeat-containing 7
  • BIRC7
  • KIAP
  • KIAPRING finger protein 50
  • Kidney inhibitor of apoptosis protein
  • livin inhibitor-of-apoptosis
  • Livin
  • Melanoma inhibitor of apoptosis protein
  • ML-IAP
  • MLIAPlivin inhibitor of apoptosis
  • ML-IAPmliap
  • RNF50LIVINbaculoviral IAP repeat-containing protein 7

Background

The inhibitor of apoptosis (IAP) family of proteins regulates programmed cell death triggered by various stimuli. All IAPs have at least one baculovirus IAP repeat (BIR) motif that is essential for their anti-apoptotic activity. Recently, Kasof and Gomes (1), Vucic et al. and Ashhab et al. have identified a novel human inhibitor of apoptosis protein (IAP) family member termed Livin or ML-IAP, which contains a single baculoviral IAP repeat (BIR) domain and a COOH-terminal RING finger domain. Livin gene has two splicing variants that contain open reading frames of 298 and 280 amino acids and both contained a single copy of baculovirus IAP repeat (BIR) and RING domain. Both of the isoforms inhibit TNFa induced apoptosis in Jurkat cells. Livin expression is low in adult tissues. It is relatively expressed at a higher level in developmental tissues and in many cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for Livin Antibody (NB100-56548)(22)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(16)
All Applications
Filter By Species
Human
(11)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NB100-56548 Applications Species
Wu S, Xu Q, Sheng W et al. A novel role for Livin in the response to ultraviolet B radiation and pterygium development Int J Mol Med 2020-01-28 [PMID: 32124942] (WB, Human) WB Human
Tchoghandjian A, Souberan A, Tabouret E et al. Inhibitor of apoptosis protein expression in glioblastomas and their in vitro and in vivo targeting by SMAC mimetic GDC-0152 Cell Death Dis 2016-08-05 [PMID: 27490930] (WB, Human) WB Human
Shiloach T, Berens C, Danke C et al. tLivin Displays Flexibility by Promoting Alternative Cell Death Mechanisms. PLoS ONE. 2014-06-25 [PMID: 24960127] (WB, Human)

Details:
Figs 1B, 2A, 2B, 3B, 3C, 5C, 6C: MelA1, 293T, or A549 cells transfected with truncated Liven (tLiven) constructs. The specificity of the Liven mAb was validated in tLiven transfected cells by WB in Figs 1B, 2A, 2B, 3B, 3C, 5C, 6C.
WB Human
Liu X, Wang A, Gao H et al. Expression and role of the inhibitor of apoptosis protein livin in chemotherapy sensitivity of ovarian carcinoma. Int J Oncol. 2012-09-01 [PMID: 22766624]
Lazar I, Perlman R, Lotem M et al. The clinical effect of the inhibitor of apopotosis protein livin in melanoma. Oncology. 2012-01-01 [PMID: 22441029]
Slagsvold JE, Pettersen CH, StOrvold GL et al. DHA alters expression of target proteins of cancer therapy in chemotherapy resistant SW620 colon cancer cells. Nutr Cancer. 2010-01-01 [PMID: 20574922] (WB, Human)

Details:
Livin/BIRC7/kIAP (IMG-347A). WB: Human colon adenocarcinoma (SW620) cell line treated with docosahexaenoic acid (DHA), Fig 2B.
WB Human
Lazar I, Yaacov B, Shiloach T et al. The oncolytic activity of Newcastle disease virus NDV-HUJ on chemoresistant primary melanoma cells is dependent on the proapoptotic activity of the inhibitor of apoptosis protein Livin. J Virol. 2010-01-01 [PMID: 19864394] (WB, Human)

Details:
WB (primary human melanoma cells); Figs 1a, 2, 3, 4a. Note: The specificity of the livin antibody was validated using livin siRNA in Fig 4a.
WB Human
Fakler M, Loeder S, Vogler M et al. Small molecule XIAP inhibitors cooperate with TRAIL to induce apoptosis in childhood acute leukemia cells and overcome Bcl-2-mediated resistance. Blood. 2009-02-19 [PMID: 19036706] (WB, Human)

Details:
WB (human leukemia cell lines), Fig. 1A.
WB Human
Wang H, Tan SS, Wang XY et al. Silencing livin gene by siRNA leads to apoptosis induction, cell cycle arrest, and proliferation inhibition in malignant melanoma LiBr cells. Acta Pharmacol Sin. 2007-12-01 [PMID: 18031611] (WB, Human)

Details:
WB (malignant melanoma human cell line LiBr), Fig 1B (siRNA validated).
WB Human
Crnkovic-Mertens I, Wagener N, Semzow J et al. Targeted inhibition of Livin resensitizes renal cancer cells towards apoptosis. Cell Mol Life Sci. 2007-05-01 [PMID: 17437058] (Human) Human
Show All 22 Publications.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Blogs on Livin.

The Ins and Outs of Survivin
By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of ...  Read full blog post.

Survivin - an inhibitor of apoptosis protein
Survivin is an anti-apoptotic protein which is the smallest protein within a large family of proteins including X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, NAIP, and Livin. Survivin is responsible for a wide range of basic cellula...  Read full blog post.

Survivin is thrivin'
The survivin anti-apoptotic protein is the smallest member of a large family of proteins such as X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, Livin, and NAIP. Survivin regulates basic physiological events such as the cell cycle, tumor...  Read full blog post.

Livin: On a Prayer
Livin is a member of the inhibitor of apoptosis proteins (IAP) family that regulates programmed cell death. The Livin protein contains a single baculovirus IAP repeat (BIR) essential for function, along with a COOH-terminal RING-type zinc finger domai...  Read full blog post.

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Bioinformatics

Gene Symbol BIRC7
Entrez
Uniprot