Livin Antibody (88C570)

Images

 
Western Blot: Livin Antibody (88C570) [NB100-56548] - detection of Livin in A) Livin transfected cell lysate and B) Molt4 cell lysate. A protein band of apprximate molecular weight of 31 kDa is detected using this ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
88C570
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Livin Antibody (88C570) Summary

Immunogen
Recombinant human livin protein was used as immunogen.
Specificity
The Livin antibody, clone 88C570 has been shown to recognize both Livin alpha (~39 kDa) and Livin beta (~37 kDa) isoforms (Nachmias et al, 2003). 2. The antibody also recognizes the 30 kDa (p30-Livin alpha) and 28 kDa (p28-Livin beta) apoptotic cleavage fragments of Livin alpha and beta, respectively (Nachmias et al, 2003). Apoptotic cleavage fragments of Livin have also been referred to as truncated livin (tLivin) (Nachmias et al, 2007). 3. The specificity of the antibody has been validated using Livin shRNA (Quiping et al, 2004).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BIRC7
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Immunocytochemistry/Immunofluorescence 2-5 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence see Nachmias et al, 2007
Publications
Read Publications using
NB100-56548 in the following applications:

  • 1 publication
  • WB
    15 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Livin Antibody (88C570)

  • baculoviral IAP repeat containing 7
  • baculoviral IAP repeat-containing 7
  • BIRC7
  • KIAP
  • KIAPRING finger protein 50
  • Kidney inhibitor of apoptosis protein
  • livin inhibitor-of-apoptosis
  • Livin
  • Melanoma inhibitor of apoptosis protein
  • ML-IAP
  • MLIAPlivin inhibitor of apoptosis
  • ML-IAPmliap
  • RNF50LIVINbaculoviral IAP repeat-containing protein 7

Background

The inhibitor of apoptosis (IAP) family of proteins regulates programmed cell death triggered by various stimuli. All IAPs have at least one baculovirus IAP repeat (BIR) motif that is essential for their anti-apoptotic activity. Recently, Kasof and Gomes (1), Vucic et al. and Ashhab et al. have identified a novel human inhibitor of apoptosis protein (IAP) family member termed Livin or ML-IAP, which contains a single baculoviral IAP repeat (BIR) domain and a COOH-terminal RING finger domain. Livin gene has two splicing variants that contain open reading frames of 298 and 280 amino acids and both contained a single copy of baculovirus IAP repeat (BIR) and RING domain. Both of the isoforms inhibit TNFa induced apoptosis in Jurkat cells. Livin expression is low in adult tissues. It is relatively expressed at a higher level in developmental tissues and in many cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Livin Antibody (NB100-56548)(21)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(15)
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Filter By Species
Human
(10)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using NB100-56548 Applications Species
Tchoghandjian A, Souberan A, Tabouret E et al. Inhibitor of apoptosis protein expression in glioblastomas and their in vitro and in vivo targeting by SMAC mimetic GDC-0152 Cell Death Dis Aug 5 2016 [PMID: 27490930] (WB, Human) WB Human
Shiloach T, Berens C, Danke C et al. tLivin Displays Flexibility by Promoting Alternative Cell Death Mechanisms. PLoS ONE. 2014 Jun 25 [PMID: 24960127] (WB, Human)

Details:
Figs 1B, 2A, 2B, 3B, 3C, 5C, 6C: MelA1, 293T, or A549 cells transfected with truncated Liven (tLiven) constructs. The specificity of the Liven mAb was validated in tLiven transfected cells by WB in Figs 1B, 2A, 2B, 3B, 3C, 5C, 6C.
WB Human
Liu X, Wang A, Gao H et al. Expression and role of the inhibitor of apoptosis protein livin in chemotherapy sensitivity of ovarian carcinoma. Int J Oncol. 2012 Sep [PMID: 22766624]
Lazar I, Perlman R, Lotem M et al. The clinical effect of the inhibitor of apopotosis protein livin in melanoma. Oncology. 2012 [PMID: 22441029]
Slagsvold JE, Pettersen CH, StOrvold GL et al. DHA alters expression of target proteins of cancer therapy in chemotherapy resistant SW620 colon cancer cells. Nutr Cancer. 2010 [PMID: 20574922] (WB, Human)

Details:
Livin/BIRC7/kIAP (IMG-347A). WB: Human colon adenocarcinoma (SW620) cell line treated with docosahexaenoic acid (DHA), Fig 2B.
WB Human
Lazar I, Yaacov B, Shiloach T et al. The oncolytic activity of Newcastle disease virus NDV-HUJ on chemoresistant primary melanoma cells is dependent on the proapoptotic activity of the inhibitor of apoptosis protein Livin. J Virol. 2010 Jan [PMID: 19864394] (WB, Human)

Details:
WB (primary human melanoma cells); Figs 1a, 2, 3, 4a. Note: The specificity of the livin antibody was validated using livin siRNA in Fig 4a.
WB Human
Fakler M, Loeder S, Vogler M et al. Small molecule XIAP inhibitors cooperate with TRAIL to induce apoptosis in childhood acute leukemia cells and overcome Bcl-2-mediated resistance. Blood. 2009 Feb 19 [PMID: 19036706] (WB, Human)

Details:
WB (human leukemia cell lines), Fig. 1A.
WB Human
Wang H, Tan SS, Wang XY et al. Silencing livin gene by siRNA leads to apoptosis induction, cell cycle arrest, and proliferation inhibition in malignant melanoma LiBr cells. Acta Pharmacol Sin. 2007 Dec [PMID: 18031611] (WB, Human)

Details:
WB (malignant melanoma human cell line LiBr), Fig 1B (siRNA validated).
WB Human
Crnkovic-Mertens I, Wagener N, Semzow J et al. Targeted inhibition of Livin resensitizes renal cancer cells towards apoptosis. Cell Mol Life Sci. 2007 May [PMID: 17437058] (Human) Human
Kleinberg L, Lie AK, FlOrenes VA et al. Expression of inhibitor-of-apoptosis protein family members in malignant mesothelioma. Hum Pathol. 2007 Jul [PMID: 17350081] (WB, Human)

Details:
WB (frozen effusions from patients with malignant mesothelioma, WM35 human melanoma cells), Fig 1.
WB Human
Show All 21 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Livin Antibody (NB100-56548) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56548AF405
Alexa Fluor 488 Labeled NB100-56548AF488
Alexa Fluor 647 Labeled NB100-56548AF647
Alexa Fluor 700 Labeled NB100-56548AF700
Biotin Labeled NB100-56548B
DyLight 350 Labeled NB100-56548UV
DyLight 405 Labeled NB100-56548V
DyLight 488 Labeled NB100-56548G
DyLight 550 Labeled NB100-56548R
DyLight 650 Labeled NB100-56548C
DyLight 680 Labeled NB100-56548FR
DyLight 755 Labeled NB100-56548IR
HRP Labeled NB100-56548H

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Bioinformatics Tool for Livin Antibody (NB100-56548)

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Research Areas for Livin Antibody (NB100-56548)

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Blogs on Livin.

Survivin - an inhibitor of apoptosis protein
Survivin is an anti-apoptotic protein which is the smallest protein within a large family of proteins including X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, NAIP, and Livin. Survivin is responsible for a wide range of basic cellula...  Read full blog post.

Survivin is thrivin'
The survivin anti-apoptotic protein is the smallest member of a large family of proteins such as X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, Livin, and NAIP. Survivin regulates basic physiological events such as the cell cycle, tumor...  Read full blog post.

Livin: On a Prayer
Livin is a member of the inhibitor of apoptosis proteins (IAP) family that regulates programmed cell death. The Livin protein contains a single baculovirus IAP repeat (BIR) essential for function, along with a COOH-terminal RING-type zinc finger domai...  Read full blog post.

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Bioinformatics

Gene Symbol BIRC7
Entrez
Uniprot