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HAF016). Specific bands were detected for CD47 at approximately 45-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
5-001-A, open histogram), followed by Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010)." class="big_lightbox" target="_blank">
4546-SA), immobilized onto a microplate, supports the adhesion of the human erythrocytes in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human SIRP-a (2 µg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human CD47 Poly-clonal Antibody (Catalog # AF4670). The adhesion was maximally inhibited (70-100%) by 2 µg/mL of the antibody." class="big_lightbox" target="_blank">
HAF016). Specific bands were detected for CD47 at approximately 50 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
HAF019). Specific bands were detected for CD47 at approximately 40-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
HAF017). Specific bands were detected for CD47 at approximately 40-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
HAF017). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Cell surface and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">
4546-SA) is immobilized at 0.1 µg/mL (100 µL/well), Recombinant Human CD47 Fc Chimera Avi-tag (Catalog # AVI4670) binds with an ED50 of 6-48 ng/mL." class="big_lightbox" target="_blank">
9378-SA) was measured at a concentration range between 6.85 nM and 3.51 µM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.134 µM. " class="big_lightbox" target="_blank">
CD47 antigen, also known as integrin associated protein (IAP), has a very broad tissue distribution. CD47 is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. There are approximately 50.000 CD47 molecules per erythrocyte. The glycoprotein is deficient in erythrocytes of the rare Rh null phenotype. CD47 is a heavily N glycosylated cell membrane glycoprotein of apparent molecular weight 47-52 kDa. It may play a role as a signaltransducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells.
![Western Blot CD47 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/CD47_AF4670_Western_Blot_15788.jpg)
![Western Blot CD47 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/CD47_AF1866_Western_Blot_17249.jpg)
![N/A CD47 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_CD47_DY467005_ELISA_2465.jpg)
![Bioactivity CD47 [Biotin]](http://images.novusbio.com/images/protein/CD47_AVI4670_2660.jpg)
![Flow Cytometry CD47 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/CD47_AF4670_Flow_Cytometry_8258.jpg)
![Immunohistochemistry CD47 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/CD47_AF4670_Immunohistochemistry_6841.jpg)
![Neutralization CD47 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/CD47_AF4670_Block_Neutralize_11560.jpg)
![Knockout Validated CD47 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/CD47_AF4670_Knockout_Validated_22577.jpg)
![Western Blot CD47 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af4670_human-cd47-affinity-purified-polyclonal-ab-4120241240512.jpg)
![Immunocytochemistry/ Immunofluorescence CD47 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af4670_human-cd47-affinity-purified-polyclonal-ab-41202412403566.jpg)
![Western Blot CD47 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af4670_human-cd47-affinity-purified-polyclonal-ab-41202412403560.jpg)
![Immunocytochemistry/ Immunofluorescence CD47 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af4670_human-cd47-affinity-purified-polyclonal-ab-41202412394925.jpg)
![Immunocytochemistry/ Immunofluorescence CD47 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af4670_human-cd47-affinity-purified-polyclonal-ab-4120241240244.jpg)
![Western Blot CD47 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af4670_human-cd47-affinity-purified-polyclonal-ab-1752025024046.jpg)
![Western Blot CD47 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/CD47_AF1866_Western_Blot_20826.jpg)
![Western Blot CD47 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1866_mouse-rat-cd47-affinity-purified-polyclonal-ab-41202412403512.jpg)
![Immunohistochemistry CD47 Antibody [Unconjugated]](http://images.novusbio.com/images/af1866_mouse-rat-cd47-affinity-purified-polyclonal-ab-immunohistochemistry-31102022135216.jpg)
![Western Blot CD47 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1866_mouse-rat-cd47-affinity-purified-polyclonal-ab-4120241240210.jpg)
![Immunocytochemistry/ Immunofluorescence CD47 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1866_mouse-rat-cd47-affinity-purified-polyclonal-ab-41202412402537.jpg)
![Immunocytochemistry/ Immunofluorescence CD47 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1866_mouse-rat-cd47-affinity-purified-polyclonal-ab-41202412405119.jpg)
![Western Blot CD47 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1866_mouse-rat-cd47-affinity-purified-polyclonal-ab-41202412403567.jpg)
![Immunocytochemistry/ Immunofluorescence CD47 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1866_mouse-rat-cd47-affinity-purified-polyclonal-ab-41202412401290.jpg)
![Immunocytochemistry/ Immunofluorescence CD47 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1866_mouse-rat-cd47-affinity-purified-polyclonal-ab-4120241240257.jpg)
![Immunocytochemistry/ Immunofluorescence CD47 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1866_mouse-rat-cd47-affinity-purified-polyclonal-ab-41202412394921.jpg)
![Immunocytochemistry/ Immunofluorescence CD47 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1866_mouse-rat-cd47-affinity-purified-polyclonal-ab-41202412401282.jpg)
![Immunocytochemistry/ Immunofluorescence CD47 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1866_mouse-rat-cd47-affinity-purified-polyclonal-ab-41202412403516.jpg)
![Immunohistochemistry CD47 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1866_mouse-rat-cd47-affinity-purified-polyclonal-ab-41202412403526.jpg)
![SDS-Page CD47 [Biotin]](http://images.novusbio.com/fullsize/protein/CD47_AVI4670_2610.jpg)
![Surface Plasmon Resonance (SPR) CD47 [Biotin]](http://images.novusbio.com/images/avi4670_recombinant-human-cd47-fc-chimera-avi-tag-protein-cf-surface-plasmon-resonance-spr-362024152449.jpg)
