CD45RC Antibody (MIL15) - BSA Free

Flow Cytometry: CD45RC Antibody (MIL15) [NB100-65001] - Figure A. Purified mouse anti porcine CD45RC detected with goat anti mouse IgM PE and mouse IgG1 FITC isotype control. Figure B. Purified mouse anti porcine CD45RC detected with goat anti mouse IgM PE and mouse anti porcine CD3 FITC. All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Flow Cytometry: CD45RC Antibody (MIL15) [NB100-65001] - Figure A. FITC conjugated mouse anti porcine CD3 and purified mouse IgM isotype control detected with goat anti mouse IgM PE. Figure B. FITC conjugated mouse anti porcine CD3 and purified mouse anti porcine CD45RC detected with goat anti mouse IgM. All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

• Reactivity: Po
• Applications: Flow, IHC
• Clone: MIL15
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

CD45RC Antibody (MIL15) - BSA Free Summary

• Description: Novus Biologicals Mouse CD45RC Antibody (MIL15) - BSA Free (NB100-65001) is a monoclonal antibody validated for use in IHC and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Cells isolated from porcine mesenteric lymph node
• Specificity: Recognizes a restricted CD45 epitope encoded by the CD45 C exon.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPRC
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry Neat
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tissue culture supernatant
• Preservative: 0.09% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for CD45RC Antibody (MIL15) - BSA Free

• B220
• CD45 antigen
• CD45R
• EC 3.1.3.48
• L-CA
• LY5
• protein tyrosine phosphatase, receptor type, C
• receptor-type tyrosine-protein phosphatase C
• T200 glycoprotein
• T200 leukocyte common antigen
• T200receptor type, c polypeptide

Background

CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognizing a common epitope on all of the isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPRC