CD45RA Antibody (PTPRC/818) - IHC-Prediluted

Images

 
Immunohistochemistry-Paraffin: CD45RA Antibody (PTPRC/818) - IHC-Prediluted [NBP2-48441] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RA Mouse Monoclonal Antibody (PTPRC/818).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P
Clone
PTPRC/818
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
IHC-Prediluted

Order Details

CD45RA Antibody (PTPRC/818) - IHC-Prediluted Summary

Immunogen
Recombinant full-length human CD45RA protein
Localization
Cell surface
Marker
Leucocyte Marker
Specificity
Recognizes a protein of 205kDa-220kDa, identified as CD45RA. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PTPRC
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. Positive control: Tonsil. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Alternate Names for CD45RA Antibody (PTPRC/818) - IHC-Prediluted

  • B220
  • CD45 antigen
  • CD45R
  • EC 3.1.3.48
  • L-CA
  • LY5
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • T200 glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47957AF350
Alexa Fluor 405 NBP2-47957AF405
Alexa Fluor 488 NBP2-47957AF488
Alexa Fluor 532 NBP2-47957AF532
Alexa Fluor 594 NBP2-47957AF594
Alexa Fluor 647 NBP2-47957AF647
Alexa Fluor 700 NBP2-47957AF700
Alexa Fluor 750 NBP2-47957AF750
Allophycocyanin NBP2-47957APC
Allophycocyanin/Cy7 NBP2-47957APCCY7
Biotin NBP2-47957B
DyLight 350 NBP2-47957UV
DyLight 405 NBP2-47957V
DyLight 488 NBP2-47957G
DyLight 550 NBP2-47957R
DyLight 594 NBP2-47957DL594
DyLight 650 NBP2-47957C
DyLight 680 NBP2-47957FR
DyLight 755 NBP2-47957IR
FITC NBP2-47957F
HRP NBP2-47957H
Janelia Fluor 549 NBP2-47957JF549
Janelia Fluor 646 NBP2-47957JF646
PE NBP2-47957PE
PE/Atto594 NBP2-47957PEATT594
PE/Cy5.5 NBP2-47957PECY55
PE/Cy7 NBP2-47957PECY7
PerCP NBP2-47957PCP

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Bioinformatics

Gene Symbol PTPRC