CD45RA Antibody (MSVA-472M) - Azide and BSA Free

• Reactivity: Hu
• Applications: IHC
• Clone: MSVA-472M
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

CD45RA Antibody (MSVA-472M) - Azide and BSA Free Summary

• Description:

  This antibody is highly specific and optimized for use in Immunohistochemistry.

  It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

  Positive Control
  Postive control:Tonsil: A strong membranous CD45RA immunostaining should be seen in the majority of lymphocytes.

  Negative Control
  Negitve control:Tonsil: CD45RA immunostaining should be absent in epithelial cells.

• Immunogen: Stimulated human leukocytes Swissprot P08575
• Localization: Cell Surface
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPRC
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry To be determined by the researcher.
• Application Notes: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD45RA Antibody (MSVA-472M) - Azide and BSA Free

• B220
• CD45 antigen
• CD45R
• CD45RA
• EC 3.1.3.48
• L-CA
• LY5
• protein tyrosine phosphatase, receptor type, C
• receptor-type tyrosine-protein phosphatase C
• T200 glycoprotein
• T200 leukocyte common antigen
• T200receptor type, c polypeptide

Background

The CD45 molecule is comprised of different glycoproteins ranging from 180-240 kD. Expression of CD45 is found on all hemopoietic cells. Detection of the different isoforms can distinguish between different cell forms i.e. naive T cells and memory T cells. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. The functional activity of each CD45 isotype also varies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPRC