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CD45.1 Antibody (A20) - Azide and BSA Free

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Flow Cytometry: CD45.1 Antibody (A20) [NBP1-28070] - SJL mouse splenocytes were stained with Mouse Anti-Mouse CD45.1-UNLB (SB Cat. No. 1795-01) followed by Goat Anti-Mouse IgG2a, Human ads-AF647.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
A20
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
0.5 mg/ml

CD45.1 Antibody (A20) - Azide and BSA Free Summary

Immunogen
SJL mouse thymocytes and splenocytes
Specificity
Mouse CD 45.1 (Leukocyte Common Antigen)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PTPRC
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1M BBS (pH 8.2)
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CD45.1 Antibody (A20) - Azide and BSA Free

  • B220
  • CD45 antigen
  • CD45.1
  • CD45R
  • EC 3.1.3.48
  • L-CA
  • LY5
  • Ly-5.1
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • T200 glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide

Background

The CD45 antigen is an essential regulator of leukocyte activation and development, and is now known to be a protein tyrosine phosphatase, e.g. CD45 is able to dephosphorylate the Src family of protein kinases. (1, 2) This activity is now known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling and Fc receptor stimulation. (1, 2) The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as the restricted variant CD45R, and as allelic forms CD45.1/Ly-5.1 and CD45.2/Ly-5.2. The former arises as a result of alternative splicing of different exons. The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies where resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation. (3, 4) The monoclonal antibody A20 recognizes all mouse leukocytes expressing the CD 45.1 allotype.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Array NBP1-28070

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Bioinformatics

Gene Symbol PTPRC