CD45.1 Antibody (A20)


Flow Cytometry: CD45.1 Antibody (A20) [NBP1-28070] - Analysis using the FITC conjugate of NBP1-28070. Staining of less or equal to 1 ug/10^6 SJL spleen cells with mouse anti-mouse CD45.1-FITC, following which small more

Product Details

Reactivity MuSpecies Glossary
Applications Flow, IHC, IHC-Fr, IHC-P, IP
0.5 mg/ml

CD45.1 Antibody (A20) Summary

Mouse CD 45.1 (Leukocyte Common Antigen)
Mouse CD 45.1 (Leukocyte Common Antigen)
IgG2a Kappa
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
0.1M BBS (pH 8.2)
No Preservative
0.5 mg/ml
Protein A or G purified


  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Reactivity Notes

Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.


Inquire about low endotoxin, azide-free CD45.1 antibody.

Alternate Names for CD45.1 Antibody (A20)

  • B220
  • CD45 antigen
  • CD45.1
  • CD45R
  • EC
  • L-CA
  • LY5
  • Ly-5.1
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • T200 glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide


The CD45 antigen is an essential regulator of leukocyte activation and development, and is now known to be a protein tyrosine phosphatase, e.g. CD45 is able to dephosphorylate the Src family of protein kinases. (1, 2) This activity is now known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling and Fc receptor stimulation. (1, 2) The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as the restricted variant CD45R, and as allelic forms CD45.1/Ly-5.1 and CD45.2/Ly-5.2. The former arises as a result of alternative splicing of different exons. The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies where resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation. (3, 4) The monoclonal antibody A20 recognizes all mouse leukocytes expressing the CD 45.1 allotype.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Species: Hu
Applications: Flow, Func, In vitro, In vivo, Flow-CS
Species: Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, InhibTFunc
Species: Hu, Mu
Applications: WB, Flow
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rb(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Species: Hu
Applications: Flow
Species: Hu
Applications: WB, IHC, IHC-P
Species: Mu
Applications: WB, Flow, IHC
Species: Mu
Applications: Flow, IHC, IHC-Fr, IHC-P, IP

Publications for CD45.1 Antibody (NBP1-28070) (0)

There are no publications for CD45.1 Antibody (NBP1-28070).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CD45.1 Antibody (NBP1-28070) (0)

There are no reviews for CD45.1 Antibody (NBP1-28070). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD45.1 Antibody (NBP1-28070) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-28070AF405
Alexa Fluor 488 Labeled NBP1-28070AF488
Alexa Fluor 647 Labeled NBP1-28070AF647
Alexa Fluor 700 Labeled NBP1-28070AF700
Allophycocyanin Labeled NBP1-28070APC
Biotin Labeled NBP1-43642
DyLight 350 Labeled NBP1-28070UV
DyLight 405 Labeled NBP1-28070V
DyLight 405LS Labeled NBP1-28070V3
DyLight 488 Labeled NBP1-28070G
DyLight 680 Labeled NBP1-28070FR
DyLight 755 Labeled NBP1-28070IR
FITC Labeled NBP1-28071
PE Labeled NBP1-28070PE
PerCP Labeled NBP1-28070PCP

Additional CD45.1 Antibody (A20) Products

CD45.1 NBP1-28070

Related Products by Gene

Bioinformatics Tool for CD45.1 Antibody (NBP1-28070)

Discover related pathways, diseases and genes to CD45.1 Antibody (NBP1-28070). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CD45.1 Antibody (NBP1-28070)

Discover more about diseases related to CD45.1 Antibody (NBP1-28070).

Pathways for CD45.1 Antibody (NBP1-28070)

View related products by pathway.

PTMs for CD45.1 Antibody (NBP1-28070)

Learn more about PTMs related to CD45.1 Antibody (NBP1-28070).

Research Areas for CD45.1 Antibody (NBP1-28070)

Find related products by research area.

Blogs on CD45.1

There are no specific blogs for CD45.1, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol PTPRC

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP1-28070 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought