CD45.1 Antibody (A20) [FITC]

Images

 
Flow Cytometry: CD45.1 Antibody (A20) [FITC] [NBP1-28071] - Less or equal to 1 ug/10^6 SJL spleen cells were stained with mouse anti-mouse CD45.1-FITC, following which small lymphocytes were gated and analyzed on a flow ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, IHC-Fr, IHC-P, IP
Clone
A20
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

CD45.1 Antibody (A20) [FITC] Summary

Immunogen
SJL mouse thymocytes and splenocytes
Specificity
Mouse CD 45.1 (Leukocyte Common Antigen)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PTPRC
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry <= 1 ug/10^6 cells
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for CD45.1 Antibody (A20) [FITC]

  • B220
  • CD45 antigen
  • CD45.1
  • CD45R
  • EC 3.1.3.48
  • L-CA
  • LY5
  • Ly-5.1
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • T200 glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide

Background

The CD45 antigen is an essential regulator of leukocyte activation and development, and is now known to be a protein tyrosine phosphatase, e.g. CD45 is able to dephosphorylate the Src family of protein kinases. (1, 2) This activity is now known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling and Fc receptor stimulation. (1, 2) The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as the restricted variant CD45R, and as allelic forms CD45.1/Ly-5.1 and CD45.2/Ly-5.2. The former arises as a result of alternative splicing of different exons. The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies where resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation. (3, 4) The monoclonal antibody A20 recognizes all mouse leukocytes expressing the CD 45.1 allotype.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP1-28070AF405
Alexa Fluor 488 NBP1-28070AF488
Alexa Fluor 647 NBP1-28070AF647
Alexa Fluor 700 NBP1-28070AF700
Allophycocyanin NBP1-28070APC
DyLight 350 NBP1-28070UV
DyLight 405 NBP1-28070V
DyLight 488 NBP1-28070G
DyLight 680 NBP1-28070FR
DyLight 755 NBP1-28070IR
FITC NBP1-28071
Janelia Fluor 549 NBP1-28070JF549
Janelia Fluor 646 NBP1-28070JF646
PE NBP1-28070PE
PerCP NBP1-28070PCP

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Array NBP1-28071

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Bioinformatics

Gene Symbol PTPRC