CD45.1 Antibody (A20) [FITC]

Flow Cytometry: CD45.1 Antibody (A20) [FITC] [NBP1-28071] - Less or equal to 1 ug/10^6 SJL spleen cells were stained with mouse anti-mouse CD45.1-FITC, following which small lymphocytes were gated and analyzed on a flow cytometer

• Reactivity: Mu
• Applications: Flow, IHC, IHC-Fr, IHC-P, IP
• Clone: A20
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

CD45.1 Antibody (A20) [FITC] Summary

• Immunogen: SJL mouse thymocytes and splenocytes
• Specificity: Mouse CD 45.1 (Leukocyte Common Antigen)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPRC
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry <= 1 ug/10^6 cells
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for CD45.1 Antibody (A20) [FITC]

• B220
• CD45 antigen
• CD45.1
• CD45R
• EC 3.1.3.48
• L-CA
• LY5
• Ly-5.1
• protein tyrosine phosphatase, receptor type, C
• receptor-type tyrosine-protein phosphatase C
• T200 glycoprotein
• T200 leukocyte common antigen
• T200receptor type, c polypeptide

Background

The CD45 antigen is an essential regulator of leukocyte activation and development, and is now known to be a protein tyrosine phosphatase, e.g. CD45 is able to dephosphorylate the Src family of protein kinases. (1, 2) This activity is now known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling and Fc receptor stimulation. (1, 2) The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as the restricted variant CD45R, and as allelic forms CD45.1/Ly-5.1 and CD45.2/Ly-5.2. The former arises as a result of alternative splicing of different exons. The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies where resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation. (3, 4) The monoclonal antibody A20 recognizes all mouse leukocytes expressing the CD 45.1 allotype.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPRC