CD43/Sialophorin Antibody (W3/13)

Images

 
Western Blot: CD43/Sialophorin Antibody (W3/13) [NB100-64992] - Western blot shows lysates of rat lymph node, rat spleen tissue, and rat thymus tissue. PVDF membrane was probed with 0.5 ug/mL of Mouse Anti-Rat CD43 ...read more
Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (W3/13) [NB100-64992] - CD43 was detected in immersion fixed paraffin-embedded sections of rat thymus using Mouse Anti-Rat CD43 Monoclonal Antibody (Catalog # ...read more
Flow Cytometry: CD43/Sialophorin Antibody (W3/13) [NB100-64992] - Staining of rat splenocytes using CD43 FITC.

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr, IHC-P
Clone
W3/13
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

CD43/Sialophorin Antibody (W3/13) Summary

Immunogen
Rat thymocyte membrane glycoproteins
Localization
Type I membrane protein.
Specificity
Rat CD43 cell surface antigen.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SPN
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/ml
  • Flow Cytometry 1:25-1:100
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Use in Western blot reported in scientific literature (PMID 7504163).
Publications
Read Publications using
NB100-64992 in the following applications:

  • 1 publication
  • WB
    1 publication

Reactivity Notes

Reacts with Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD43/Sialophorin Antibody (W3/13)

  • CD43 antigen
  • CD43
  • CD43)
  • Galactoglycoprotein
  • GALGP
  • GPL115
  • Leukocyte sialoglycoprotein
  • Leukosialin
  • LSN
  • Ly-48
  • sialophorin (gpL115, leukosialin, CD43)
  • Sialophorin
  • SPN

Background

CD43 is one of the major glycoproteins of thymocytes and T lymphocytes, it plays a role in the physicochemical properties of the T cell surface and in lectin binding. CD43 presents carbohydrate ligands to selectins. It has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. The antigen is a counter receptor for SN/Siglec1. During T cell activation CD43 is actively removed from the T cell antigen presenting cell contact site suggesting a negative regulatory role in adaptive immune response. CD43 is expressed by the majority of leucocytes, with the exception of most B cells. However CD43 expression is seen in some cases of B cell lymphocytic lymphoma and centrocytic lymphoma. CD43 cell surface antigen, also known as leukosialin and sialophorin. CD43 is a 95kD heavily glycosylated glycoprotein expressed by all leucocytes with the exception of B lymphocytes. This product is routinely tested in flow cytometry on rat splenocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD43/Sialophorin Antibody (NB100-64992)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NB100-64992AF405
Alexa Fluor 488 NB100-64992AF488
Alexa Fluor 647 NB100-64992AF647
Alexa Fluor 700 NB100-64992AF700
Allophycocyanin NB100-64992APC
DyLight 350 NB100-64992UV
DyLight 405 NB100-64992V
DyLight 488 NB100-64992G
DyLight 680 NB100-64992FR
DyLight 755 NB100-64992IR
PE NB100-64992PE
PerCP NB100-64992PCP

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Bioinformatics

Gene Symbol SPN