CD43/Sialophorin Antibody (84-3C1) - Chimeric - Azide and BSA Free

Western Blot: CD43/Sialophorin Antibody (84-3C1) - Chimeric [NBP3-12075] - Daudi(A) cell lysate, K562(B) cell lysate and human bone marrow(C) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 84-3C1f (NBP3-12075) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: CD43/Sialophorin Antibody (84-3C1) - Chimeric [NBP3-12075] - Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 84-3C1 (NBP3-12075) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12075, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Flow Cytometry: CD43/Sialophorin Antibody (84-3C1) - Chimeric - Azide and BSA Free [NBP3-12075] - K562 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12075 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: 84-3C1
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

CD43/Sialophorin Antibody (84-3C1) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit CD43/Sialophorin Antibody (84-3C1) - Chimeric - Azide and BSA Free (NBP3-12075) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was raised by immunizng mice with stimulated human leukocytes.
• Specificity: This antibody recognizes CD43/Sialophorin which is a predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SPN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CD43/Sialophorin Antibody (84-3C1) - Chimeric - Azide and BSA Free

• CD43 antigen
• CD43
• CD43)
• Galactoglycoprotein
• GALGP
• GPL115
• Leukocyte sialoglycoprotein
• Leukosialin
• LSN
• Ly-48
• sialophorin (gpL115, leukosialin, CD43)
• Sialophorin
• SPN

Background

CD43 is a key glycoprotein of thymocytes and T lymphocytes, which affects the physicochemical properties of the T-cell surface and plays a role in lectin binding. This antibody contains an extended rodlike structure that may protrude above the glycocalyx of the cell, allowing accessibility of multiple glycan chains for binding. During T-cell activation CD43 is actively removed from the T-cell-antigen-presenting cell contact site, thus suggesting CD43's role in negative regulation of the adaptive immune response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPN