CD40 Ligand/TNFSF5 Antibody [Unconjugated]

Images

 
Recombinant Mouse CD40 Ligand/TNFSF5 (Catalog # 8230-CL) induces proliferation in mouse splenic B cells in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by ...read more
CD40 Ligand/TNFSF5 was detected in immersion fixed mouse splenocytes either stimulated with PHA (left panel) or untreated (right panel) using Goat Anti-Mouse CD40 Ligand/TNFSF5 Antigen Affinity-purified Polyclonal ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

CD40 Ligand/TNFSF5 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD40 Ligand/TNFSF5
Glu61-Leu260
Accession # P27548
Specificity
Detects mouse CD40 Ligand/TNFSF5 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CD40LG
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunocytochemistry 5-15 ug/mL
  • Neutralization 0.08-0.4 ug/mL
Publications
Read Publications using
AF1163 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD40 Ligand/TNFSF5 Antibody [Unconjugated]

  • CD154 antigen
  • CD154
  • CD40 antigen ligand
  • CD40 Ligand
  • CD40L
  • CD40-L
  • CD40LG
  • CD40LIGM
  • gp39
  • hCD40L
  • HIGM1
  • T-B cell-activating molecule
  • T-BAM
  • T-cell antigen Gp39
  • TNF-related activation protein
  • TNFSF5
  • TNFSF5IMD3
  • TRAP
  • TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
  • tumor necrosis factor (ligand) superfamily member 5
  • Tumor necrosis factor ligand superfamily member 5

Background

CD40 ligand (CD40L), also known as CD154, TNFSF5, TRAP or gp39, is a 260 amino acid (aa) type II transmembrane glycoprotein belonging to the TNF family. Murine CD40L consists of a 22 aa cytoplasmic domain, a 24 aa transmembrane domain, and 214 aa extracellular domain bearing a single glycosylation site (1, 2). CD40L is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, including NK cells, mast cells, basophils and eosinophils. Murine CD40L shares 78% amino acid sequence identity with human CD40L. Native bioactive soluble CD40L exists. Soluble human trimeric CD40L secreted by stimulated T cells has been shown to be generated by proteolysis in the microsomes (3). Both membrane bound and soluble CD40L induce similar effects on B cells (3, 4). The receptor of CD40L is CD40, a type I transmembrane glycoprotein belonging to the TNF receptor family. CD40 is expressed on B lymphocytes, monocytes, dendritic cells, and thymic epithelium. Although all monomeric, dimeric and trimeric forms of soluble CD40L can bind to CD40, the soluble trimeric form of CD40L has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members (2). The genetic defect in the hyper-IgM syndrome is due to point mutations or deletions of the gene encoding the CD40L, which prevent CD40L from interacting with CD40 (5-7). CD40L mediates a range of activities on B cells including induction of activation-associated surface antigen, entry into the cell cycle, isotype switching, Ig secretion, and memory generation (8, 9). CD40-CD40L interaction also plays important roles in monocyte activation and dendritic cell maturation (10).

  1. Armitage, R.J. et al. (1992) Nature 357:80.
  2. Hollenbaugh, D. et al. (1992) EMBO J. 11:4313.
  3. Fabienne, P. et al. (1996) J. Biol. Chem. 271:5965.
  4. Fabienne, P. et al. (1996) Eur. J. Immunol 26:725.
  5. Arrufo, A. et al. (1993) Cell 72:291.
  6. Hill, A. and N. Chapel et al. (1993) Nature 361:494.
  7. Korthauer, U. et al. (1993) Nature 361:539.
  8. Spriggs, M.K. et al. (1992) J. Exp. Med. 176:1543.
  9. Fanslow, W.C. et al. (1994) Seminars in Immunology 6:267.
  10. Kooten, C.V. and J. Banchereau (2000) J. Leukoc. Biol. 67:2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD40 Ligand/TNFSF5 Antibody (AF1163)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ELISA Development, ICC, IHC-P, Western Blot.


Filter By Application
ELISA Development
(2)
ICC
(1)
IHC-P
(1)
Western Blot
(3)
All Applications
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Human
(2)
Mouse
(5)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF1163 Applications Species
S de Silva, G Fromm, CW Shuptrine, K Johannes, A Patel, KJ Yoo, K Huang, TH Schreiber CD40 Enhances Type I Interferon Responses Downstream of CD47 Blockade, Bridging Innate and Adaptive Immunity Cancer Immunol Res, 2019;0(0):. 2019 [PMID: 31852716] (Western Blot, Human) Western Blot Human
MS Russell, A Muralidhar, L Larocque, J Cao, Y Deschambau, J Varga, SN Thulasi Ra, X Li Identification and characterisation of the CD40-ligand of Sigmodon hispidus PLoS ONE, 2018;13(7):e0199067. 2018 [PMID: 30052641] (ELISA Development, Mouse) ELISA Development Mouse
Gupta, Sachin, Termini, James M, Raffa, Francesc, Williams, Cindi-An, Kornbluth, Richard, Stone, Geoffrey Vaccination with a fusion protein that introduces HIV-1 gag antigen into a multitrimer CD40L construct results in enhanced CD8+ T cell responses and protection from viral challenge by vaccinia-gag. J Virol, 2014;88(3):1492-501. 2014 [PMID: 24227853] (Western Blot, Mouse) Western Blot Mouse
Akiyama T, Shimo Y, Yanai H, Qin J, Ohshima D, Maruyama Y, Asaumi Y, Kitazawa J, Takayanagi H, Penninger JM, Matsumoto M, Nitta T, Takahama Y, Inoue J The tumor necrosis factor family receptors RANK and CD40 cooperatively establish the thymic medullary microenvironment and self-tolerance. Immunity, 2008;29(3):423-37. 2008 [PMID: 18799149] (IHC-P, Mouse) IHC-P Mouse
Liu J, Yu Q, Stone GW, Yue FY, Ngai N, Jones RB, Kornbluth RS, Ostrowski MA CD40L expressed from the canarypox vector, ALVAC, can boost immunogenicity of HIV-1 canarypox vaccine in mice and enhance the in vitro expansion of viral specific CD8+ T cell memory responses from HIV-1-infected and HIV-1-uninfected individuals. Vaccine, 2008;26(32):4062-72. 2008 [PMID: 18562053] (Western Blot, Mouse) Western Blot Mouse
Koguchi Y, Thauland TJ, Slifka MK, Parker DC Preformed CD40 ligand exists in secretory lysosomes in effector and memory CD4+ T cells and is quickly expressed on the cell surface in an antigen-specific manner. Blood, 2007;110(7):2520-7. 2007 [PMID: 17595332] (ICC, Mouse) ICC Mouse
Field AE, Wagage S, Conrad SM Reduced pathology following infection with transgenic Leishmania major expressing murine CD40 ligand. Infect. Immun., 2007;75(6):3140-9. 2007 [PMID: 17403867] (ELISA Development, Human) ELISA Development Human

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for CD40 Ligand/TNFSF5 Antibody (AF1163). (Showing 1 - 1 of 1 FAQs).

  1. I am looking for paired ABs for use in detecting CD40L (rat) by ELISA. Do you have such a reagent(s) available?
    • Unfortunately we currently do not have any CD40 Ligand antibody pairs that have been validated in Rat. The only antibody pairs we offer for CD40L are validated in Human. I apologize for this inconvenience. If you would be interested in testing one of our products in Rat I'd like to invite you to participate in our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

Secondary Antibodies

 

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Other Available Formats

Biotin BAF1163

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Bioinformatics

Gene Symbol CD40LG
Uniprot