CD40 Ligand/TNFSF5 Antibody (TRAP1.3.6) Summary
Mouse myeloma cell line transfected with human CD40L (CD154)
TRAP1.3.6 recognises the human CD154 cell surface antigen, a 32kD glycoprotein also known as CD40 ligand. CD154 is expressed on activated T lymphocytes, predominantly CD4 +ve and also on some basophils and mast cells. TRAP1.3.6 binds to CD154 at an epitope distinct from the CD40 binding site. Immunogen: Mouse myeloma cell line transfected with human CD40L (CD154)
Protein A or G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Flow Cytometry 1:10-1:1000
The antibody can be used for flow cytometry.Flow Cytometry: Use 10ul of the suggested working dilution (1/10-1/50) to label 106 cells in 100ul.
Human,Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS pH 7.4
0.09% Sodium Azide
Protein A or G purified
Alternate Names for CD40 Ligand/TNFSF5 Antibody (TRAP1.3.6)
- CD154 antigen
- CD40 antigen ligand
- CD40 Ligand
- T-B cell-activating molecule
- T-cell antigen Gp39
- TNF-related activation protein
- TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
- tumor necrosis factor (ligand) superfamily member 5
- Tumor necrosis factor ligand superfamily member 5
CD40 ligand (CD40L) is a 33 kDa type II membrane glycoprotein expressed mainly on the cell surface of activated T lymphocytes, but also exists as a soluble form extracellularly. CD40L is the ligand for CD40, a member of the TNF superfamily, which is expressed on the cell surface of B cells, macrophages/monocytes, dendritic cells, vascular endothelial cells, and epithelial cells. CD40L plays an important role in B cell proliferation, antibody class switching, modulation of apoptosis in the germinal center through interaction with B cells expressing CD40, and activation of CD4+ T cells. Mutation within the CD40L gene is linked to hyper IgM syndrome, an X linked immunodeficiency disease that is characterized by elevated level of serum IgM and decreased level of other isotypes.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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Product General Protocols
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FAQs for CD40 Ligand/TNFSF5 Antibody (NBP1-42527). (Showing 1 - 1 of 1 FAQ).
I am looking for paired ABs for use in detecting CD40L (rat) by ELISA. Do you have such a reagent(s) available?
- Unfortunately we currently do not have any CD40 Ligand antibody pairs that have been validated in Rat. The only antibody pairs we offer for CD40L are validated in Human. I apologize for this inconvenience. If you would be interested in testing one of our products in Rat I'd like to invite you to participate in our Innovators Reward Program. Please contact us at firstname.lastname@example.org with any questions regarding this program.
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