CD36/SR-B3 Antibody (324205) [Alexa Fluor™ Plus 405]

• Reactivity: Mu
• Clone: 324205
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor Plus 405

CD36/SR-B3 Antibody (324205) [Alexa Fluor™ Plus 405] Summary

• Specificity: Detects mouse CD36/SR-B3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CD36 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD36/SR-B3 Antibody (324205) [Alexa Fluor™ Plus 405]

• BDPLT10
• CD_antigen: CD36
• CD36 antigen (collagen type I receptor, thrombospondin receptor)
• CD36 antigen
• CD36 molecule (thrombospondin receptor)
• CD36
• CHDS7
• cluster determinant 36
• Collagen R
• FAT
• Fatty acid translocase
• Glycoprotein IIIb
• GP3B
• GP4
• GPIIIb
• GPIV
• PAS IV
• PAS-4 protein
• PAS-4
• PASIV
• Platelet collagen receptor
• platelet glycoprotein 4
• SCARB3
• scavenger receptor class B, member 3
• SRB3
• SR-B3
• Thrombospondin R
• Thrombospondin receptor

Background

CD36 (alternatively known as platelet membrane glycoprotein IV (GPIV), thrombospondin receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3)) is an 88 kDa, integral membrane glycoprotein that belongs to the class B scavenger receptor family (1, 2). The molecule is described as being ditopic, with two transmembrane segments connected by an extracellular loop (3). Mouse CD36 is synthesized as a 472 amino acid (aa) protein that contains a 6 aa N‑terminal cytoplasmic domain, a 22 aa N‑terminal transmembrane segment, a 420 aa extracellular “loop”, a 22 aa C‑terminal transmembrane segment, and a 9 aa C‑terminal cytoplasmic tail (4). Both cytoplasmic tails are palmitoylated, with the C‑terminal tail involved in oxidized LDL binding (5, 6). With respect to the extracellular loop, the N‑terminal region is believed to bind both thrombospondin-1 and Plasmodium-infected erythrocytes. Other ligands for CD36 include long-chain fatty acids, collagen, phospholipids and apoptotic cells (1). The extracellular loop of mouse CD36 shares 94%, 92%, 84% and 84% aa sequence identity with the extracellular loops of rat, hamster, human and bovine CD36, respectively. Cells known to express CD36 include capillary endothelium, adipocytes, skeletal muscle cells, intestinal epithelium, smooth muscle cells and hematopoietic cells such as RBC’s, platelets and monocytes (1). On the surface of cells, CD36 is suggested to exist as a dimer in response to ligation (7). CD36 is reported to regulate fatty uptake, act as an angiogenic with TSP-1, and participate in the clearance of apoptotic phagocytes (1, 8).

1. Febbraio, M. et al. (2001) J. Clin. Invest. 108:795. 
2. Silverstein, R.L. and M. Febbraio (2000) Curr. Opin. Lipid. 11:483. 
3. Gruarin, P. et al. (2000) Biochem. Biophys. Res. Commun. 275:446. 
4. Endemann, G. et al. (1993) J. Biol. Chem. 268:11811. 
5. Malaud, E. et al. (2002) Biochem. J. 364:507.
6. Tao, N. et al. (1996) J. Biol. Chem. 271:22315.
7. Daviet, L. et al. (1997) Thromb. Haemost. 78:897.
8. Simantov, R. and R.L. Silverstein (2003) Front. Biosci. 8:s874.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.