CD36/SR-B3 Antibodies: Novus Biologicals

AF2519

CD36/SR-B3 Antibody [Unconjugated]

(1 Review)

100 μg

AF2519

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_lightbox">

J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse CD36/SR-B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2519, filled histogram) or isotype control antibody (Catalog # <a class=

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." alt="J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse CD36/SR-B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2519, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

CD36/SR-B3 was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse CD36/SR-B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2519) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

$Liver GADD45 beta controls liver fatty acid handling by cytosolic FABP1 retentionA, BMale 12‐weeks‐old wild‐type (WT; C57Bl/6J) or obese/diabetic (db/db; BKS.Cg‐m+/+ Lepr DB/J) mice with (Ad‐G45b OE) or without (Ad‐NC) prior liver‐restricted GADD45 beta over‐expression were fasted and insulin was injected with livers harvested shortly thereafter and subsequently liver proteins were subjected to immunoblotting for insulin signalling proteins including phosphoprotein kinase B (PKB/Akt; A) and glycogen synthase kinase beta (GSK3b; B). Inserts show representative blots (n = 6/group).C, DRepresentative immunoblots (C) and relative abundance quantifications (D; n = 6) of proteins and phosphoproteins including light chain 3 isoform B (LC3B), S6 kinase 1 (S6K1), p42/44 mitogen activated protein kinase (ERK1/2), eukaryotic initiation factor 2 alpha (eIF2a), glucose regulated protein 78 (GRP78/HSPA5), fatty acid transport protein 2 (FATP2/SLC27A2), cluster determinant 36 (CD36/FAT) and fatty acid binding protein 1 (FABP1) from fasted GADD45 beta +/+ (WT) or GADD45 beta −/− (KO) mice.ERepresentative immunoblots of HNF4a (nuclear marker), BCKDE1A (mitochondrial marker), NTCP (microsomal marker) and ARG1 (cytosolic marker) from liver whole tissue lysate (W) as well as fractionated organelles/intraceuular structures including nuclei (N), mitochondria (MT), microsomes (MS) and cytoplasm (C), from GADD45 beta +/+ (WT) and GADD45 beta −/− (KO) mice.Data information: Data are mean ± SEM. The statistical test used and respective P‐value outputs can be found in Appendix Table S1. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27137487), licensed under a CC-BY license. Not internally tested by R&D Systems.$

$Liver GADD45 beta controls liver fatty acid handling by cytosolic FABP1 retentionA, BMale GADD45 beta +/+ (WT; n = 16) or GADD45 beta −/− (KO; n = 15) mice fasted for 24 h (fasted) with (AV‐G45b OE) or without (AV‐NC) liver‐restricted GADD45 beta over‐expression (n = 7–8/group). Liver mRNA expression of Gadd45b (A) as well as fatty acid metabolic genes (B) encompassing transport (Slc28a2, Slc27a5, Cd36), intracellular binding (Fabp1, Dbi) and metabolism (Acly, Dgat1, Atgl, Hsl).CRepresentative immunoblots of FATP2, CD36, FABP1 and GADD45 beta from liver whole tissue lysate (W) as well as fractionated organelles/intracellular structures including nuclei (N), mitochondria (MT), microsomes (MS) and cytoplasm (C), from GADD45 beta +/+ (WT) and GADD45 beta −/− (KO) mice.DQuantified band densities of FABP1 enrichment from fractions in C (n = 4/group).ELiver fraction enrichment of FABP1 from male GADD45 beta +/+ (WT) or GADD45 beta −/− (KO) mice fasted for 24 h with (AD‐G45b OE) or without (AD‐NC) liver‐restricted GADD45 beta over‐expression (n = 4/group). Insert shows a representative FABP1 immunoblot.FLiver fraction enrichment of FABP1 from obese/diabetic male db/db mice fasted for 24 h with (AD‐G45b OE) or without (AD‐NC) liver‐restricted GADD45 beta over‐expression (n = 4/group). Insert shows a representative FABP1 immunoblot.GFABP1 and GADD45B immunoblots from Flag immunoprecipitations (IP‐FLAG) or mock IP (IP‐HA) from liver input samples from mice with (AD‐G45b OE) or without (AD‐NC) liver‐restricted GADD45 beta over‐expression. Shown is a representative immunoblot from 3 separate experiments using 3 different input samples per condition.H–JLiver tissue long‐chain acyl‐CoA (LC‐acyl‐CoA) concentrations were determined in GADD45 beta +/+ (WT) or GADD45 beta −/− (KO) mice (H; n = 6/group) with (AD‐G45b OE) or without (AD‐NC) liver‐restricted GADD45 beta over‐expression (I; n = 5/group). Liver LC‐acyl‐CoA concentrations were determined in wild‐type (WT; C57Bl/6J) or obese/diabetic (db/db; BKS.Cg‐m+/+ Lepr DB/J) mice with (AD‐G45b OE) or$

HIIT improves lipid metabolism of skeletal muscle in T2DM mice. (A,D) Protein expressions of ACC, HMGCR, CPT-1 alpha , CD36, and internal control alpha -tubulin in skeletal muscle. (B,C,E,F) Quantification of proteins described in (A,D) with normalization to protein levels of alpha -tubulin. All data are presented as mean ± SEM. n = 4 per group; *p < 0.05, **p < 0.01. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32922365), licensed under a CC-BY license. Not internally tested by R&D Systems.

Absence of Endo1 increases cell surface CD36 levels and lipid uptake in adipocytes.(A) Cell surface expression of CD36 in mature white adipocytes. **P < 0.01 versus WT. Results are expressed as mean ± SEM (n = 4). Two-tailed t test. (B) Total CD36 expression in gonadal adipose tissue (GAT) and s.c. adipose tissue (SAT). The molecular weights of protein markers are indicated (kDa). Results are expressed as mean ± SEM (n = 5). (C) Immunofluorescence images of CD36 cell surface expression in differentiated white adipocytes (left panel). Level of cellular fluorescence determined by corrected total cell fluorescence per area (CTCF/area). Results are expressed as mean ± SEM (n = 9). ***P < 0.005 versus WT. Two-tailed t test (right panel). Scale bar: 20 μm. (D) Lipid uptake in differentiated adipocytes, mature adipocytes, and differentiated myotubes. Results are expressed as mean ± SEM (n = 5–6). *P < 0.05; **P < 0.01; ****P < 0.001 versus basal. †P < 0.05; ††P < 0.01; ††††P < 0.001 versus WT. One-way ANOVA with Bonferroni correction. Image collected and cropped by CiteAb from the following open publication (//insight.jci.org/articles/view/168418), licensed under a CC-BY license. Not internally tested by R&D Systems.

$Endospanin 1 directly interacts with CD36.(A) Detection of Flag-CD36 in 6Myc-Endo1 immunoprecipitate from HEK293T cells coexpressing 6Myc-Endo1 and Flag-CD36 compared with HEK293T cells transfected with Flag-CD36 alone. Representative blots of 3 independent experiments. (B) Detection of 6Myc-Endo1 after Flag-CD36 immunoprecipitation from HEK293T cells coexpressing 6Myc-Endo1 and Flag-CD36 compared with HEK293T cells transfected with 6Myc-Endo1 alone. Representative blots of 3 independent experiments. (C) Kinetics of Endo1 and CD36 protein expression in differentiated adipocytes during differentiation of adipocyte precursors isolated from the stromal vascular fraction of the s.c. adipose tissue of WT mice into mature white adipocytes. Results are expressed as mean ± SEM of 8 independent experiments. One representative Western blot is shown. (D) Confocal immunofluorescence detection and colocalization of Endo1 (rabbit anti-Endo1) and CD36 (goat anti-CD36) in differentiated adipocytes. Nuclei (blue) are stained with fluorescent DAPI dye. Scale bar: 20 μm. Representative images of 4 independent experiments. (E) Detection of endogenous Endo1 and CD36 after immunoprecipitation with Endo1 or CD36 antibodies from lysates of visceral adipose tissue (VAT), gonadal adipose tissue (GAT), and s.c. adipose tissue (SAT) of WT and KO mice. Endo1-KO adipocytes and tissues were used as negative controls. The molecular weights of protein markers are indicated (kDa). Representative blots of 2 independent experiments. Image collected and cropped by CiteAb from the following open publication (//insight.jci.org/articles/view/168418), licensed under a CC-BY license. Not internally tested by R&D Systems.$

Induced levels of lipogenic proteins after insulin stimulation were lower in DKO hepatocytes.(A) Expression levels of FASN, ACC, and FAT/CD36 in hepatocytes at 360 min after insulin stimulation were analyzed by immunoblotting. (B) Expression levels of lipogenic molecules are presented by measuring band intensities after correction with those of beta -Actin. Open and closed bars indicate WT and DKO hepatocytes, respectively. The numbers of mice examined were WT, n = 3; DKO, n = 4. Data are expressed as means ± S.D. *P<0.05, **P<0.01 and ***P<0.001 as compared between WT and DKO hepatocytes. #P<0.05 and ##P<0.01 as compared between basal and insulin stimulation. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36827398), licensed under a CC-BY license. Not internally tested by R&D Systems.

Impact of peroxisomal defect on the expression of phagocytosis-related genes in WT and mutant (Abcd1−/−Abcd2−/− and Acox1−/−) BV-2 cells. (A) Schematic representation of the main actors of phagocytosis. (B) Venn diagrams illustrating the up- and downregulated phagocytosis-related DEGs in Abcd1−/−Abcd2−/− and Acox1−/− non-stimulated BV-2 cells (DataSet GSE200022). Genes whose expression level was measured at the protein level are boxed. (C) Pie chart displaying the number of up- or downregulated phagocytosis-related DEGs after a 24-h LPS treatment (Log2FC > 1, AdjP Value < 0.05) in WT, Abcd1−/−Abcd2−/−, and Acox1−/− BV-2 cells (DataSet GSE237635). (D) Venn diagrams representing the LPS response of these DEGs (three independent batches of BV-2 cells for each genotype). (E) Heat map representing RNA-Seq gene expression of these DEGs at 24 h after LPS stimulation in WT, Abcd1−/−Abcd2−/−, and Acox1−/− BV-2 cells. (F) Representative image of Western blotting analysis (three independent experiments) of the expression levels of CD36, FCGR2B, MRC1, TLR2, and TLR4 in WT, Abcd1−/−Abcd2−/−, and Acox1−/− BV-2 cells untreated (–) or treated with LPS for 24 h (+). Expected molecular weights are indicated. Source data are available online for this figure. Image collected and cropped by CiteAb from the following open publication (//www.frontiersin.org/articles/10.3389/fnmol.2023.1299314/full), licensed under a CC-BY license. Not internally tested by R&D Systems.

2519-CD) was serially diluted and captured by Goat Anti-Mouse CD36/SR‑B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2519) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CD36/SR‑B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2519) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Mouse CD36/SR‑B3 Fc Chimera (Catalog # <a class=

2519-CD) was serially diluted and captured by Goat Anti-Mouse CD36/SR‑B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2519) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CD36/SR‑B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2519) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

2519-CD) was serially diluted and captured by Goat Anti-Mouse CD36/SR‑B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2519) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CD36/SR‑B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2519) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

2519-CD) was serially diluted and captured by Goat Anti-Mouse CD36/SR‑B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2519) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CD36/SR‑B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2519) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2519U-100UG
Alexa Fluor 405	AF2519V-100UG
Alexa Fluor 488	AF2519G-100UG
Alexa Fluor 532	AF2519X-100UG
Alexa Fluor 594	AF2519T-100UG
Alexa Fluor 647	AF2519R-100UG
Alexa Fluor 700	AF2519N-100UG
Alexa Fluor 750	AF2519S-100UG
Alexa Fluor Plus 405	AF2519AFP405-100UG
Alexa Fluor Plus 488	AF2519AFP488-100UG
Alexa Fluor Plus 555	AF2519AFP555-100UG
Alexa Fluor Plus 594	AF2519AFP594-100UG
Alexa Fluor Plus 647	AF2519AFP647-100UG
Alexa Fluor Plus 680	AF2519AFP680-100UG
Allophycocyanin	AF2519A
Biotin	BAF2519
CoraFluor 1	AF2519CL1
DyLight 350	AF2519D
DyLight 405	AF2519E
DyLight 488	AF2519K
DyLight 550	AF2519L
DyLight 594	AF2519M
DyLight 650	AF2519W
DyLight 680	AF2519Y
DyLight 755	AF2519Z
FITC	AF2519F
HRP	AF2519H
Janelia Fluor 525	AF2519JF525
Janelia Fluor 549	AF2519I
Janelia Fluor 646	AF2519J
Janelia Fluor 669	AF2519JF669
PE	AF2519P
PerCP	AF2519C
mFluor Violet 450 SE	AF2519MFV450
mFluor Violet 500 SE	AF2519MFV500
mFluor Violet 610 SE	AF2519MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2519-SP

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AF1955

CD36/SR-B3 Antibody [Unconjugated]

(14 Publications)

100 μg

AF1955

HAF019). A specific band was detected for CD36/SR-B3 at approximately 85-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD36/SR-B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1955) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF019). A specific band was detected for CD36/SR-B3 at approximately 85-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD36/SR-B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1955) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD36/SR-B3 at approximately 85-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for CD36/SR-B3 at approximately 140 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CD36/SR-B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1955) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of human adipose tissue, loaded at 0.2 mg/mL. A specific band was detected for CD36/SR-B3 at approximately 125 kDa (as indicated) using 50 µg/mL of Goat Anti-Human CD36/SR-B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1955) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1955U-100UG
Alexa Fluor 405	AF1955V-100UG
Alexa Fluor 488	AF1955G-100UG
Alexa Fluor 532	AF1955X-100UG
Alexa Fluor 594	AF1955T-100UG
Alexa Fluor 647	AF1955R-100UG
Alexa Fluor 700	AF1955N-100UG
Alexa Fluor 750	AF1955S-100UG
Alexa Fluor Plus 405	AF1955AFP405-100UG
Alexa Fluor Plus 488	AF1955AFP488-100UG
Alexa Fluor Plus 555	AF1955AFP555-100UG
Alexa Fluor Plus 594	AF1955AFP594-100UG
Alexa Fluor Plus 647	AF1955AFP647-100UG
Alexa Fluor Plus 680	AF1955AFP680-100UG
Allophycocyanin	AF1955A
Biotin	AF1955B
CoraFluor 1	AF1955CL1
DyLight 350	AF1955D
DyLight 405	AF1955E
DyLight 488	AF1955K
DyLight 550	AF1955L
DyLight 594	AF1955M
DyLight 650	AF1955W
DyLight 680	AF1955Y
DyLight 755	AF1955Z
FITC	AF1955F
HRP	AF1955H
Janelia Fluor 525	AF1955JF525
Janelia Fluor 549	AF1955I
Janelia Fluor 646	AF1955J
Janelia Fluor 669	AF1955JF669
PE	AF1955P
PerCP	AF1955C
mFluor Violet 450 SE	AF1955MFV450
mFluor Violet 500 SE	AF1955MFV500
mFluor Violet 610 SE	AF1955MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1955-SP

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MAB25191

CD36/SR-B3 Antibody (324205)

(5 Publications)

100 μg

MAB25191

MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." class="big_lightbox">

MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." alt="J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse CD36/SR-B3 Monoclonal Antibody (Catalog # MAB25191, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." class="big_thumb" />

CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

CD36/SR-B3 was detected in immersion fixed frozen sections of mouse heart using Rat Anti-Mouse CD36/SR-B3 Monoclonal Antibody (Catalog # MAB25191) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

CD36/SR-B3 was detected in immersion fixed frozen sections of mouse thymus using Rat Anti-Mouse CD36/SR-B3 Monoclonal Antibody (Catalog # MAB25191) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB25191U-100UG
Alexa Fluor 405	FAB25191V-100UG
Alexa Fluor 488	FAB25191G-100UG
Alexa Fluor 532	FAB25191X
Alexa Fluor 594	FAB25191T-100UG
Alexa Fluor 647	FAB25191R-100UG
Alexa Fluor 700	FAB25191N-100UG
Alexa Fluor 750	FAB25191S-100UG
Alexa Fluor Plus 405	FAB25191AFP405-100UG
Alexa Fluor Plus 488	FAB25191AFP488-100UG
Alexa Fluor Plus 555	FAB25191AFP555-100UG
Alexa Fluor Plus 594	FAB25191AFP594-100UG
Alexa Fluor Plus 647	FAB25191AFP647-100UG
Alexa Fluor Plus 680	FAB25191AFP680-100UG
Allophycocyanin/Cy7	FAB25191APCCY7
Biotin	FAB25191B
CoraFluor 1	FAB25191CL1
DyLight 350	FAB25191D
DyLight 405	FAB25191E
DyLight 488	FAB25191K
DyLight 550	FAB25191L
DyLight 594	FAB25191M
DyLight 650	FAB25191W
DyLight 680	FAB25191Y
DyLight 755	FAB25191Z
FITC	FAB25191F
HRP	FAB25191H
Janelia Fluor 525	FAB25191JF525
Janelia Fluor 549	FAB25191I
Janelia Fluor 646	FAB25191J
Janelia Fluor 669	FAB25191JF669
PE	FAB25191P
PE/Atto594	FAB25191PEATT594
PE/Cy5.5	FAB25191PECY55
PE/Cy7	FAB25191PECY7
PerCP	FAB25191C
mFluor Violet 450 SE	FAB25191MFV450
mFluor Violet 500 SE	FAB25191MFV500
mFluor Violet 610 SE	FAB25191MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB25191-SP
Carrier Free	FAB25191V-100UG
Carrier Free	FAB25191U-100UG
Carrier Free	FAB25191T-100UG
Carrier Free	FAB25191S-100UG
Carrier Free	FAB25191R-100UG
Carrier Free	FAB25191N-100UG
Carrier Free	FAB25191G-100UG

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MAB19552

CD36/SR-B3 Antibody (877346) [Unconjugated]

(1 Publication)

100 μg

MAB19552

HAF018). A specific band was detected for CD36/SR-B3 at approximately 85-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CD36/SR-B3 Mono-clonal Antibody (Catalog # MAB19552) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF018). A specific band was detected for CD36/SR-B3 at approximately 85-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CD36/SR-B3 Mono-clonal Antibody (Catalog # MAB19552) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD36/SR-B3 at approximately 85-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

VC001). Tissue was stained with DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

CD36/SR-B3 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human CD36/SR-B3 Monoclonal Antibody (Catalog # MAB19552) at 5 µg/mL for 1 hour at room temperature followed by incubation with Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC001). Tissue was stained with DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Simple Western lane view shows lysates of human platelets, loaded at 0.5 mg/mL. A specific band was detected for CD36/SR‑B3 at approximately 138 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human CD36/SR‑B3 Monoclonal Antibody (Catalog # MAB19552) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB19552U-100UG
Alexa Fluor 405	FAB19552V-100UG
Alexa Fluor 488	FAB19552G-100UG
Alexa Fluor 532	FAB19552X-100UG
Alexa Fluor 594	FAB19552T-100UG
Alexa Fluor 647	FAB19552R-100UG
Alexa Fluor 700	FAB19552N-100UG
Alexa Fluor 750	FAB19552S-100UG
Alexa Fluor Plus 405	FAB19552AFP405-100UG
Alexa Fluor Plus 488	FAB19552AFP488-100UG
Alexa Fluor Plus 555	FAB19552AFP555-100UG
Alexa Fluor Plus 594	FAB19552AFP594-100UG
Alexa Fluor Plus 647	FAB19552AFP647-100UG
Alexa Fluor Plus 680	FAB19552AFP680-100UG
Allophycocyanin	FAB19552A
Biotin	FAB19552B
CoraFluor 1	FAB19552CL1
DyLight 350	FAB19552D
DyLight 405	FAB19552E
DyLight 488	FAB19552K
DyLight 550	FAB19552L
DyLight 594	FAB19552M
DyLight 650	FAB19552W
DyLight 680	FAB19552Y
DyLight 755	FAB19552Z
FITC	FAB19552F
HRP	FAB19552H
Janelia Fluor 525	FAB19552JF525
Janelia Fluor 549	FAB19552I
Janelia Fluor 646	FAB19552J
Janelia Fluor 669	FAB19552JF669
PE	FAB19552P
PerCP	FAB19552C
mFluor Violet 450 SE	FAB19552MFV450
mFluor Violet 500 SE	FAB19552MFV500
mFluor Violet 610 SE	FAB19552MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB19552-SP

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MAB2519

CD36/SR-B3 Antibody (324216)

(1 Review)

(5 Publications)

100 μg

MAB2519

$Inulin and SCFA prevent the onset of diet-induced hepatic steatosis by affecting lipid metabolism in different ways. Characterization of liver tissue in male C57BL/6JRj mice that were fed a semi-synthetic low-fat diet (LF) or high-fat diets (HF) supplemented with either 10% dietary fibre (HFC: 10% cellulose; HFI: 3% cellulose + 7% inulin; HFG: 3% cellulose + 7% guar gum; depicted in green hues) or 5% SCFA (depicted in red hues) with different Ac:Pr ratios, a high acetate (HAc; 10:1 Ac:Pr) or high propionate diet (HPr; 1:2.5 Ac:Pr) for 30 weeks. (A) Representative H&E staining of hepatocytes after intervention. (B) Liver tissue weight (n = 19–21) and corresponding (C) hepatic triglyceride (TG) concentration, n = 9–11. (D) Formation of odd-chain fatty acids (OCFA) in liver phospholipid fraction, n = 8. (E) Hepatic gene expression, (F) respective western blots with corresponding load control (GAPDH) and (G) analysis of protein expression (normalized to LF-diet as set to a value of 1), n = 4–8. Analysis of (H) citrate synthase (CS) and (I) cytochrome c oxidase (COX) activity in liver, n = 5–8. (J) Hepatic fatty acid synthase (FASN) activity and (K) calculated de novo lipogenesis (DNL)-Index by measurement of long-chain fatty acid amount in liver phospholipids, n = 8. Data are mean + SEM. *P < 0.05; **P < 0.01; ***P < 0.001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28733671), licensed under a CC-BY license. Not internally tested by R&D Systems.$

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB2519U-100UG
Alexa Fluor 405	FAB2519V-100UG
Alexa Fluor 488	FAB2519G-100UG
Alexa Fluor 532	FAB2519X-100UG
Alexa Fluor 594	FAB2519T-100UG
Alexa Fluor 647	FAB2519R-100UG
Alexa Fluor 700	FAB2519N-100UG
Alexa Fluor 750	FAB2519S-100UG
Alexa Fluor Plus 405	FAB2519AFP405-100UG
Alexa Fluor Plus 488	FAB2519AFP488-100UG
Alexa Fluor Plus 555	FAB2519AFP555-100UG
Alexa Fluor Plus 594	FAB2519AFP594-100UG
Alexa Fluor Plus 647	FAB2519AFP647-100UG
Alexa Fluor Plus 680	FAB2519AFP680-100UG
Allophycocyanin	FAB2519A
Biotin	FAB2519B
CoraFluor 1	FAB2519CL1
DyLight 350	FAB2519D
DyLight 405	FAB2519E
DyLight 488	FAB2519K
DyLight 550	FAB2519L
DyLight 594	FAB2519M
DyLight 650	FAB2519W
DyLight 680	FAB2519Y
DyLight 755	FAB2519Z
FITC	FAB2519F
HRP	FAB2519H
Janelia Fluor 525	FAB2519JF525
Janelia Fluor 549	FAB2519I
Janelia Fluor 646	FAB2519J
Janelia Fluor 669	FAB2519JF669
PE	FAB2519P
PerCP	FAB2519C
mFluor Violet 450 SE	FAB2519MFV450
mFluor Violet 500 SE	FAB2519MFV500
mFluor Violet 610 SE	FAB2519MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB2519-SP

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MAB1955

CD36/SR-B3 Antibody (255619)

(4 Publications)

100 μg

MAB1955

The cholesterol metabolism in macrophages.(A) Immunoblotting of CD36 and ABCA1 in peritoneal macrophages and foam cells and (B) The density of the protein band was quantitated. (C) Immunoblotting of HSL and ATGL in peritoneal macrophages and foam cells and (D) the density of the protein bands was quantitated. (E) The rate of cholesterol ester hydrolysis in peritoneal macrophages from Plin1+/+ and Plin1-/- mice. (F) Time-course of cholesterol efflux to ApoA-I in peritoneal macrophages from Plin1+/+ and Plin1-/- mice. * p < 0.05, ** p < 0.01 for Plin-/- vs. Plin+/+. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25855981), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1955U-100UG
Alexa Fluor 405	FAB1955V-100UG
Alexa Fluor 488	FAB1955G-100UG
Alexa Fluor 532	FAB1955X-100UG
Alexa Fluor 594	FAB1955T-100UG
Alexa Fluor 647	FAB1955R-100UG
Alexa Fluor 700	FAB1955N-100UG
Alexa Fluor 750	FAB1955S-100UG
Alexa Fluor Plus 405	FAB1955AFP405-100UG
Alexa Fluor Plus 488	FAB1955AFP488-100UG
Alexa Fluor Plus 555	FAB1955AFP555-100UG
Alexa Fluor Plus 594	FAB1955AFP594-100UG
Alexa Fluor Plus 647	FAB1955AFP647-100UG
Alexa Fluor Plus 680	FAB1955AFP680-100UG
Allophycocyanin	FAB1955A
Biotin	FAB1955B
CoraFluor 1	FAB1955CL1
DyLight 350	FAB1955D
DyLight 405	FAB1955E
DyLight 488	FAB1955K
DyLight 550	FAB1955L
DyLight 594	FAB1955M
DyLight 650	FAB1955W
DyLight 680	FAB1955Y
DyLight 755	FAB1955Z
FITC	FAB1955F
HRP	FAB1955H
Janelia Fluor 525	FAB1955JF525
Janelia Fluor 549	FAB1955I
Janelia Fluor 646	FAB1955J
Janelia Fluor 669	FAB1955JF669
PE	FAB1955P
PerCP	FAB1955C
mFluor Violet 450 SE	FAB1955MFV450
mFluor Violet 500 SE	FAB1955MFV500
mFluor Violet 610 SE	FAB1955MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1955-SP

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MAB19551

CD36/SR-B3 Antibody (255606) [Unconjugated]

500 μg

MAB19551

NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

CD36/SR-B3 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Rat Anti-Human CD36/SR-B3 Monoclonal Antibody (Catalog # MAB19551) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # <a class=

NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="CD36/SR-B3 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Rat Anti-Human CD36/SR-B3 Monoclonal Antibody (Catalog # MAB19551) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB19551U-100UG
Alexa Fluor 405	FAB19551V-100UG
Alexa Fluor 488	FAB19551G-100UG
Alexa Fluor 532	FAB19551X
Alexa Fluor 594	FAB19551T-100UG
Alexa Fluor 647	FAB19551R-100UG
Alexa Fluor 700	FAB19551N-100UG
Alexa Fluor 750	FAB19551S-100UG
Alexa Fluor Plus 405	FAB19551AFP405-100UG
Alexa Fluor Plus 488	FAB19551AFP488-100UG
Alexa Fluor Plus 555	FAB19551AFP555-100UG
Alexa Fluor Plus 594	FAB19551AFP594-100UG
Alexa Fluor Plus 647	FAB19551AFP647-100UG
Alexa Fluor Plus 680	FAB19551AFP680-100UG
Allophycocyanin/Cy7	FAB19551APCCY7
Biotin	FAB19551B
CoraFluor 1	FAB19551CL1
DyLight 350	FAB19551D
DyLight 405	FAB19551E
DyLight 488	FAB19551K
DyLight 550	FAB19551L
DyLight 594	FAB19551M
DyLight 650	FAB19551W
DyLight 680	FAB19551Y
DyLight 755	FAB19551Z
HRP	FAB19551H
Janelia Fluor 525	FAB19551JF525
Janelia Fluor 549	FAB19551I
Janelia Fluor 646	FAB19551J
Janelia Fluor 669	FAB19551JF669
PE/Atto594	FAB19551PEATT594
PE/Cy5.5	FAB19551PECY55
PE/Cy7	FAB19551PECY7
PerCP	FAB19551C
mFluor Violet 450 SE	FAB19551MFV450
mFluor Violet 500 SE	FAB19551MFV500
mFluor Violet 610 SE	FAB19551MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB19551-SP
Carrier Free	FAB19551V-100UG
Carrier Free	FAB19551U-100UG
Carrier Free	FAB19551T-100UG
Carrier Free	FAB19551S-100UG
Carrier Free	FAB19551R-100UG
Carrier Free	FAB19551N-100UG
Carrier Free	FAB19551G-100UG

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FAB19554T

CD36/SR-B3 Antibody (1283D) [Alexa Fluor® 594]

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Alexa Fluor 594

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB19554U-100UG
Alexa Fluor 405	FAB19554V-100UG
Alexa Fluor 488	FAB19554G-100UG
Alexa Fluor 532	FAB19554X-100UG
Alexa Fluor 647	FAB19554R-100UG
Alexa Fluor 700	FAB19554N-100UG
Alexa Fluor 750	FAB19554S-100UG
Alexa Fluor Plus 405	FAB19554AFP405-100UG
Alexa Fluor Plus 488	FAB19554AFP488-100UG
Alexa Fluor Plus 555	FAB19554AFP555-100UG
Alexa Fluor Plus 594	FAB19554AFP594-100UG
Alexa Fluor Plus 647	FAB19554AFP647-100UG
Alexa Fluor Plus 680	FAB19554AFP680-100UG
Allophycocyanin	FAB19554A
Biotin	FAB19554B
CoraFluor 1	FAB19554CL1
DyLight 350	FAB19554D
DyLight 405	FAB19554E
DyLight 488	FAB19554K
DyLight 550	FAB19554L
DyLight 594	FAB19554M
DyLight 650	FAB19554W
DyLight 680	FAB19554Y
DyLight 755	FAB19554Z
FITC	FAB19554F
HRP	FAB19554H
Janelia Fluor 525	FAB19554JF525
Janelia Fluor 549	FAB19554I
Janelia Fluor 646	FAB19554J
Janelia Fluor 669	FAB19554JF669
PE	FAB19554P
PerCP	FAB19554C
mFluor Violet 450 SE	FAB19554MFV450
mFluor Violet 500 SE	FAB19554MFV500
mFluor Violet 610 SE	FAB19554MFV610

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FAB19554A

CD36/SR-B3 Antibody (1283D) [Allophycocyanin]

0.1 ml

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Allophycocyanin

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB19554U-100UG
Alexa Fluor 405	FAB19554V-100UG
Alexa Fluor 488	FAB19554G-100UG
Alexa Fluor 532	FAB19554X-100UG
Alexa Fluor 594	FAB19554T-100UG
Alexa Fluor 647	FAB19554R-100UG
Alexa Fluor 700	FAB19554N-100UG
Alexa Fluor 750	FAB19554S-100UG
Alexa Fluor Plus 405	FAB19554AFP405-100UG
Alexa Fluor Plus 488	FAB19554AFP488-100UG
Alexa Fluor Plus 555	FAB19554AFP555-100UG
Alexa Fluor Plus 594	FAB19554AFP594-100UG
Alexa Fluor Plus 647	FAB19554AFP647-100UG
Alexa Fluor Plus 680	FAB19554AFP680-100UG
Biotin	FAB19554B
CoraFluor 1	FAB19554CL1
DyLight 350	FAB19554D
DyLight 405	FAB19554E
DyLight 488	FAB19554K
DyLight 550	FAB19554L
DyLight 594	FAB19554M
DyLight 650	FAB19554W
DyLight 680	FAB19554Y
DyLight 755	FAB19554Z
FITC	FAB19554F
HRP	FAB19554H
Janelia Fluor 525	FAB19554JF525
Janelia Fluor 549	FAB19554I
Janelia Fluor 646	FAB19554J
Janelia Fluor 669	FAB19554JF669
PE	FAB19554P
PerCP	FAB19554C
mFluor Violet 450 SE	FAB19554MFV450
mFluor Violet 500 SE	FAB19554MFV500
mFluor Violet 610 SE	FAB19554MFV610

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AF1955AFP647

CD36/SR-B3 Antibody [Alexa Fluor™ Plus 647]

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Alexa Fluor Plus 647

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AF2519AFP405

CD36/SR-B3 Antibody [Alexa Fluor™ Plus 405]

100 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Alexa Fluor Plus 405

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FAB2519AFP555

CD36/SR-B3 Antibody (324216) [Alexa Fluor™ Plus 555]

100 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Host:

Rat Monoclonal

Host: Rat Monoclonal

Conjugate:

Alexa Fluor Plus 555

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Has Sample Size

Promotional Item

Species Glossary

CD36/SR-B3 Antibodies

CD36/SR-B3 Antibody [Unconjugated]

CD36/SR-B3 Antibody [Unconjugated]

CD36/SR-B3 Antibody (324205)

CD36/SR-B3 Antibody (877346) [Unconjugated]

CD36/SR-B3 Antibody (324216)

CD36/SR-B3 Antibody (255619)

CD36/SR-B3 Antibody (255606) [Unconjugated]

CD36/SR-B3 Antibody (1283D) [Alexa Fluor® 594]

CD36/SR-B3 Antibody (1283D) [Allophycocyanin]

CD36/SR-B3 Antibody [Alexa Fluor™ Plus 647]

CD36/SR-B3 Antibody [Alexa Fluor™ Plus 405]

CD36/SR-B3 Antibody (324216) [Alexa Fluor™ Plus 555]