CD36/SR-B3 Antibody (324205)

J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse CD36/SR‑B3 Monoclonal Antibody (Catalog # MAB25191, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

CD36/SR-B3 was detected in immersion fixed frozen sections of mouse heart using Rat Anti-Mouse CD36/SR-B3 Monoclonal Antibody (Catalog # MAB25191) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

CD36/SR-B3 was detected in immersion fixed frozen sections of mouse thymus using Rat Anti-Mouse CD36/SR-B3 Monoclonal Antibody (Catalog # MAB25191) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: Flow, IHC, CyTOF-ready
• Clone: 324205
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

CD36/SR-B3 Antibody (324205) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse CD36/SR-B3
  Gly30-Lys439
  Accession # Q08857
• Specificity: Detects mouse CD36/SR-B3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CD36 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: CD36
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD36/SR-B3 Antibody (324205)

• BDPLT10
• CD_antigen: CD36
• CD36 antigen (collagen type I receptor, thrombospondin receptor)
• CD36 antigen
• CD36 molecule (thrombospondin receptor)
• CD36
• CHDS7
• cluster determinant 36
• Collagen R
• FAT
• Fatty acid translocase
• Glycoprotein IIIb
• GP3B
• GP4
• GPIIIb
• GPIV
• PAS IV
• PAS-4 protein
• PAS-4
• PASIV
• Platelet collagen receptor
• platelet glycoprotein 4
• SCARB3
• scavenger receptor class B, member 3
• SRB3
• SR-B3
• Thrombospondin R
• Thrombospondin receptor

Background

CD36 (alternatively known as platelet membrane glycoprotein IV (GPIV), thrombospondin receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3)) is an 88 kDa, integral membrane glycoprotein that belongs to the class B scavenger receptor family (1, 2). The molecule is described as being ditopic, with two transmembrane segments connected by an extracellular loop (3). Mouse CD36 is synthesized as a 472 amino acid (aa) protein that contains a 6 aa N‑terminal cytoplasmic domain, a 22 aa N‑terminal transmembrane segment, a 420 aa extracellular “loop”, a 22 aa C‑terminal transmembrane segment, and a 9 aa C‑terminal cytoplasmic tail (4). Both cytoplasmic tails are palmitoylated, with the C‑terminal tail involved in oxidized LDL binding (5, 6). With respect to the extracellular loop, the N‑terminal region is believed to bind both thrombospondin-1 and Plasmodium-infected erythrocytes. Other ligands for CD36 include long-chain fatty acids, collagen, phospholipids and apoptotic cells (1). The extracellular loop of mouse CD36 shares 94%, 92%, 84% and 84% aa sequence identity with the extracellular loops of rat, hamster, human and bovine CD36, respectively. Cells known to express CD36 include capillary endothelium, adipocytes, skeletal muscle cells, intestinal epithelium, smooth muscle cells and hematopoietic cells such as RBC’s, platelets and monocytes (1). On the surface of cells, CD36 is suggested to exist as a dimer in response to ligation (7). CD36 is reported to regulate fatty uptake, act as an angiogenic with TSP-1, and participate in the clearance of apoptotic phagocytes (1, 8).

1. Febbraio, M. et al. (2001) J. Clin. Invest. 108:795. 
2. Silverstein, R.L. and M. Febbraio (2000) Curr. Opin. Lipid. 11:483. 
3. Gruarin, P. et al. (2000) Biochem. Biophys. Res. Commun. 275:446. 
4. Endemann, G. et al. (1993) J. Biol. Chem. 268:11811. 
5. Malaud, E. et al. (2002) Biochem. J. 364:507.
6. Tao, N. et al. (1996) J. Biol. Chem. 271:22315.
7. Daviet, L. et al. (1997) Thromb. Haemost. 78:897.
8. Simantov, R. and R.L. Silverstein (2003) Front. Biosci. 8:s874.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD36/SR-B3 Antibody (MAB25191)(5)

Publications using MAB25191

• Rediet Zewdu, Elnaz Mirzaei Mehrabad, Kelley Ingram, Pengshu Fang, Katherine L Gillis, Soledad A Camolotto et al. An NKX2-1/ERK/WNT feedback loop modulates gastric identity and response to targeted therapy in lung adenocarcinoma eLife 4/6/2021 [PMID: 33821796]
• Enos N, Takenaka H, Scott S et al. Meningeal Foam Cells and Ependymal Cells in Axolotl Spinal Cord Regeneration Front Immunol. 11/1/2019 [PMID: 31736973]
  
  Details:
  Axolotl
• Y. Zhu, K. Lyapichev, D.H. Lee, D. Motti, N.M. Ferraro, Y. Zhang et al. Macrophage Transcriptional Profile Identifies Lipid Catabolic Pathways That Can Be Therapeutically Targeted after Spinal Cord Injury The Journal of Neuroscience 3/1/2017 [PMID: 28130359]
• Nassir F, Adewole O, Brunt E, Abumrad N CD36 deletion reduces VLDL secretion, modulates liver prostaglandins, and exacerbates hepatic steatosis in ob/ob mice. J Lipid Res, 2013-08-20;54(11):2988-97. 2013-08-20 [PMID: 23964120] (IHC, Mouse)
• Nicholls HT, Kowalski G, Kennedy DJ Hematopoietic cell-restricted deletion of CD36 reduces high-fat diet-induced macrophage infiltration and improves insulin signaling in adipose tissue. Diabetes, 2011-03-04;60(4):1100-10. 2011-03-04 [PMID: 21378177] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: CD36
• Uniprot:
  • Q08857()