CD34 Antibody (SPM123)

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Immunohistochemistry-Paraffin: CD34 Antibody (SPM123) [NBP2-32933] - Formalin-fixed paraffin-embedded human tonsil stained with CD34 Monoclonal Antibody (SPM123)

Product Details

Summary
Reactivity Hu, Pm, Bv(-), Ca(-), Rt(-), Sh(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
SPM123
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

CD34 Antibody (SPM123) Summary

Immunogen
Detergent solubilized vesicular suspension prepared from human term placenta
Localization
Cell surface
Marker
Hematopoietic Stem Cell & Endothelial Marker
Specificity
This MAb recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified into three main categories, class I, class II and class III. It is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain. CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi's sarcoma, but with a lower specificity.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD34
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25-0.5ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
  • Immunoprecipitation 0.5-1ug/500ug protein lysate
Application Notes
Human Chromosome Location: 1q32.2
Mol. Weight of Antigen:90-110kDa
Immunohistochemistry (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Use in Immunohistochemistry reported in scientific literature (PMID: 30650584).
Publications
Read Publication using
NBP2-32933 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Cynomolgus Monkey, Rhesus Monkey

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
PBS with 0.05% BSA.
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A purified

Alternate Names for CD34 Antibody (SPM123)

  • CD34 antigenhematopoietic progenitor cell antigen CD34
  • CD34 molecule
  • CD34
  • HPCA1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-32933
Species: Hu, Pm, Bv(-), Ca(-), Rt(-), Sh(-)
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for CD34 Antibody (NBP2-32933)(1)

We have publications tested in 1 confirmed species: Sheep.

We have publications tested in 1 application: IHC.


Filter By Application
IHC
(1)
All Applications
Filter By Species
Sheep
(1)
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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD34 Antibody (NBP2-32933). (Showing 1 - 1 of 1 FAQ).

  1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
    • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP2-34733AF405
Alexa Fluor 488 NBP2-34733AF488
Alexa Fluor 647 NBP2-34733AF647
Alexa Fluor 700 NBP2-34733AF700
Allophycocyanin NBP2-34733APC
Biotin NBP2-34733B
DyLight 350 NBP2-34733UV
DyLight 405 NBP2-34733V
DyLight 488 NBP2-34733G
DyLight 550 NBP2-34733R
DyLight 650 NBP2-34733C
DyLight 680 NBP2-34733FR
DyLight 755 NBP2-34733IR
HRP NBP2-34733H
Janelia Fluor 549 NBP2-34733JF549
Janelia Fluor 646 NBP2-34733JF646
PE NBP2-34733PE
PerCP NBP2-34733PCP

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Blogs on CD34.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

CD68 (Cluster of differentiation 68, GP110, LAMP4, SCARD1)
CD68 belongs to a growing family of hematopoietic mucin-like molecules known as lysosomal/endosomal-associated membrane glycoproteins (LAMPs). Other LAMP family members included leukosialin, stem cell antigen CD34, and GlyCAM-1. CD68 encodes a 110-kD...  Read full blog post.

CD34 (Cluster of differentiation 34, hematopoietic progenitor cell antigen)
CD34 is a cell-surface glycoprotein type 1 transmembrane protein that belongs to the sialomucin family. CD34 comprises of an intracellular cytoplasmic domain with consensus sites for serine, threonine, tyrosine and active protein kinase C (PKC). Becau...  Read full blog post.

CD34 Serves as an Important Marker in Disease Research
CD34 is a membrane protein that aids cells in cell-cell adhesion. Although little is known about its function, CD34 is an important marker for hematopoietic stem cells (HSCs), muscle satellite cells, and endothelial cells. HSCs can be found in bone ma...  Read full blog post.

CD34 Serves as an Important Marker in Disease Research
CD34 is a cell surface glycoprotein that aids cells in cell-cell adhesion. It is expressed on endothelial cells where it is known to bind L-selectin and may aid in migration of T-cells. Moreover, it is expressed on hematopoietic stem cells (HSC), musc...  Read full blog post.

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Bioinformatics

Gene Symbol CD34
Uniprot