CD300e/LMIR6 Antibody (UP-H2) - Low Endotoxin, Azide and BSA Free

Flow (Cell Surface): CD300e/LMIR6 Antibody (UP-H2) - Low Endotoxin [NBP2-62230] - Surface staining of human peripheral blood with anti-human CD300e (UP-H2) PE.

Flow (Cell Surface): CD300e/LMIR6 Antibody (UP-H2) - Low Endotoxin [NBP2-62230] - Surface staining of human peripheral blood with anti-human CD300e (UP-H2) APC.

Separation of human monocytes (red-filled) from CD300e negative lymphocytes (black-dashed) in Analysis (surface staining) of human peripheral whole blood using anti-human CD300e (UP-H2) purified antibody (concentration in sample 4 ug/ml, GAM APC).

• Reactivity: Hu
• Applications: WB, Flow, Func, IP
• Clone: UP-H2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Low Endotoxin, Azide and BSA Free
• Concentration: 1 mg/ml

CD300e/LMIR6 Antibody (UP-H2) - Low Endotoxin, Azide and BSA Free Summary

• Immunogen: CD300e-HA-transfected cells
• Specificity: The mouse monoclonal antibody UP-H2 recognizes an extracellular epitope on CD300e / IREM-2, a 32 kDa glycoprotein expressed by mature monocytes and peripheral blood myeloid dendritic cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD300E
• Purity: Protein A purified
• Endotoxin Note: Endotoxin level is less than 0.01 EU/ug of the protein

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-4 ug/ml
  • Functional
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CD300e/LMIR6 Antibody (UP-H2) - Low Endotoxin, Azide and BSA Free

• CD300 antigen like family member E
• CD300 antigen-like family member E
• CD300e antigenPIgR-2
• CD300e molecule
• CD300e
• CD300LE
• CD300LEPIgR2
• CLM2
• CLM-2
• CLM2CMRF35-A5
• CMRF35A5
• CMRF35-like molecule 2
• Immune receptor expressed on myeloid cells 2
• IREM-2
• IREM2IREM-2
• LMIR6
• Poly-Ig receptor 2
• Polymeric immunoglobulin receptor 2

Background

CD300e / IREM-2 (immune receptor expressed by myeloid cells 2), also known as CLM2 or LMIR6, is a monomeric transmembrane glycoprotein with a single extracellular immunoglobulin-like domain. Intracellularly it associates with DAP-12, an ITAM-containing adaptor molecule. CD300e is expressed on mature monocytes and peripheral blood myeloid dendritic cells. Its crosslinking leads to release of pro-inflammatory cytokines, and increased expression of activation markers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD300E