CD3 zeta [p Tyr153] Antibody (EM-17) - BSA Free

Western Blot: CD3 zeta [p Tyr153] Antibody (EM-17) [NBP2-62192] - Reactivity of the monoclonal antibodies EM-26 (anti-CD3 zeta phospho-Tyr72) and EM-17 (anti-CD3 zeta phospho-Tyr153) with phosphorylated particular human CD3 zeta mutants. The Y1F and Y6F mutatants lack phosphotyrosine 72 and 153, respectively.

• Reactivity: Hu, Mu
• Applications: WB, Flow
• Clone: EM-17
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

CD3 zeta [p Tyr153] Antibody (EM-17) - BSA Free Summary

• Immunogen: A phospho specific peptide corresponding to the amino acids surrounding tyrosine 153 of mouse CD3 zeta linked to KLH
• Modification: p Tyr153
• Specificity: The antibody POH-1 specifically detects Cdk1 (p34Cdc2). Staining of Cdk1 reflects the proliferating potential of respective tissue.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD247
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Western Blot 2-5 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Purity: Protein A purified

Alternate Names for CD3 zeta [p Tyr153] Antibody (EM-17) - BSA Free

• CD247 antigen
• CD247 antigen, zeta subunit
• CD247 molecule
• CD247
• CD3 zeta
• CD3H
• CD3Q
• CD3z antigen, zeta polypeptide (TiT3 complex)
• CD3Z
• CD3ZT-cell receptor T3 zeta chain
• IMD25
• T3Z
• T-cell antigen receptor complex, zeta subunit of CD3
• T-cell surface glycoprotein CD3 zeta chain
• TCR Zeta Chain
• TCRZCD3-ZETA

Background

CD3 (Cluster of Differentiation 3) is a complex of proteins that associates directly with the T cell antigen receptor (TCR). Antigen binding to the TCR leads to IL-2 secretion via activation of a tyrosine phosphorylation pathway and a phospholipase C (PLC) pathway, in turn activating protein kinase C. CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Loss of the zeta chain results in the synthesis of unstable TCRs. A decrease of CD3 zeta has been described in T cells from patients with cancer, lupus and chronic infectious diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD247